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The map label for this gene is trmB

Identifier: 15672731

GI number: 15672731

Start: 756296

End: 756949

Strand: Direct

Name: trmB

Synonym: L156302

Alternate gene names: 15672731

Gene position: 756296-756949 (Clockwise)

Preceding gene: 15672730

Following gene: 15672732

Centisome position: 31.97

GC content: 35.93

Gene sequence:

>654_bases
ATGCGCGTCAGAAATCGTAAAGGGGCAGGCGAAATGCTTGCTGAAAACGCACATATCGTAGTTGAAAATCCAGCGGACTT
TAAAGGACGTTGGTCAGAACGTTTTGGAAATGATCATCCGATTCATATTGAAGTGGGTTGTGGAAAAGGAGCTTTCATCA
CAGGAATGGCAGCTCTTCATCCAGAAATAAATTACATTGCAATTGATATGCAATTATCAGTTTTATCTTATGCTTTAGAT
AAAGCAATTGAAGCAGATTTGCCAAACGTACAAATGATGCTTGTTGATGGAGCTGCTTTGAGTGAATATTTTGCTGATGG
TGAAATTGACCAAGTCTATTTGAACTTTTCTGACCCTTGGCCAAAAGGACGTCATGAAAAACGTCGTTTGACTTATAAAT
CTTTTCTTGCAACTTATGAAAAGATTTTACGTCCTGAAGGTGAAATTCATTTCAAAACTGACAATCGTGGATTGTTTGAA
TATTCTTTAGTTTCTTTAGCTAACTATGGCATGGAACTTAAAAAAGTTTGGTTAGATTTACACCAAGACGAAGAATTTGC
ACCTCAAAATGTAATGACCGAGTATGAGCAAAAATTCTCACAAAAAGGTCAAGTAATTTATCGATTAGAAGCAAAATTTT
TGCCTAAGAAGTAA

Upstream 100 bases:

>100_bases
GACCTTCCTATTTTTTGGCTTATAATTGAATAATAAAAATAAAGAAATAGAAGATCACTTTTGCTGATTTTCTATCAAAA
ATTAAAAAATTGAGGTAAAA

Downstream 100 bases:

>100_bases
ATAAGTTGCTTTCTGTCATTAAAACTAATGAAAAAAATTACTGACAGAATAAGAAAGTCTAAACCAATTTTCTGTCAGTA
AAATTAATAAGAATTTATTA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALHPEINYIAIDMQLSVLSYALD
KAIEADLPNVQMMLVDGAALSEYFADGEIDQVYLNFSDPWPKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFE
YSLVSLANYGMELKKVWLDLHQDEEFAPQNVMTEYEQKFSQKGQVIYRLEAKFLPKK

Sequences:

>Translated_217_residues
MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALHPEINYIAIDMQLSVLSYALD
KAIEADLPNVQMMLVDGAALSEYFADGEIDQVYLNFSDPWPKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFE
YSLVSLANYGMELKKVWLDLHQDEEFAPQNVMTEYEQKFSQKGQVIYRLEAKFLPKK
>Mature_217_residues
MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALHPEINYIAIDMQLSVLSYALD
KAIEADLPNVQMMLVDGAALSEYFADGEIDQVYLNFSDPWPKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFE
YSLVSLANYGMELKKVWLDLHQDEEFAPQNVMTEYEQKFSQKGQVIYRLEAKFLPKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=179, Percent_Identity=27.3743016759777, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_LACLA (Q9CHI2)

Other databases:

- EMBL:   AE005176
- PIR:   E86718
- RefSeq:   NP_266905.1
- ProteinModelPortal:   Q9CHI2
- SMR:   Q9CHI2
- GeneID:   1114375
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L156302
- NMPDR:   fig|272623.1.peg.768
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   LLAC272623:L156302-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24948; Mature: 24948

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALH
CCCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHC
PEINYIAIDMQLSVLSYALDKAIEADLPNVQMMLVDGAALSEYFADGEIDQVYLNFSDPW
CCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCEEEEEEECCCCC
PKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFEYSLVSLANYGMELKKVWLDL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHH
HQDEEFAPQNVMTEYEQKFSQKGQVIYRLEAKFLPKK
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALH
CCCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHC
PEINYIAIDMQLSVLSYALDKAIEADLPNVQMMLVDGAALSEYFADGEIDQVYLNFSDPW
CCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCEEEEEEECCCCC
PKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFEYSLVSLANYGMELKKVWLDL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHH
HQDEEFAPQNVMTEYEQKFSQKGQVIYRLEAKFLPKK
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471