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The map label for this gene is ptsH

Identifier: 15672099

GI number: 15672099

Start: 120380

End: 120646

Strand: Direct

Name: ptsH

Synonym: L120335

Alternate gene names: 15672099

Gene position: 120380-120646 (Clockwise)

Preceding gene: 15672098

Following gene: 15672100

Centisome position: 5.09

GC content: 40.82

Gene sequence:

>267_bases
ATGGCATCTAAAGAATTCCACATCGTTGTAGAAACTGGTATCCATGCACGTCCAGCTACATTGCTTGTGCATACAGCTTC
AAAATTCACATCAGAAATCACTTTGGAATACAAAGGTAAATCAGTAAACCTTAAATCAATCATGGGTGTTATGTCACTCG
GTGTTGGTCAAGGCGCTGACGTTACTATTTCAGCTGAAGGTGCAGATGCTGACGATGCAATCTCAACAATCGCTGAAACA
ATGACTAAAGAAGGACTCGCAGAATAA

Upstream 100 bases:

>100_bases
ATATTGCAAACCCTTTCGTTTTGTGGTACAATTTCAAGAGTGATAGATATTTTAGATATCGTCAATAAAAATGAAAAAAC
ATCTAAGGAGAACCATAAAA

Downstream 100 bases:

>100_bases
TCATGACAACTATGCTTAAAGGTATCGCCGCATCATCAGGTGTAGCAGTAGCTAAGGCATACTTGCTTGTCCAACCAGAT
TTGTCTTTCGAAACAAAGAC

Product: phosphocarrier protein HPr

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein

Number of amino acids: Translated: 88; Mature: 87

Protein sequence:

>88_residues
MASKEFHIVVETGIHARPATLLVHTASKFTSEITLEYKGKSVNLKSIMGVMSLGVGQGADVTISAEGADADDAISTIAET
MTKEGLAE

Sequences:

>Translated_88_residues
MASKEFHIVVETGIHARPATLLVHTASKFTSEITLEYKGKSVNLKSIMGVMSLGVGQGADVTISAEGADADDAISTIAET
MTKEGLAE
>Mature_87_residues
ASKEFHIVVETGIHARPATLLVHTASKFTSEITLEYKGKSVNLKSIMGVMSLGVGQGADVTISAEGADADDAISTIAETM
TKEGLAE

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

Organism=Escherichia coli, GI1788494, Length=89, Percent_Identity=42.6966292134831, Blast_Score=68, Evalue=9e-14,
Organism=Escherichia coli, GI1788755, Length=81, Percent_Identity=43.2098765432099, Blast_Score=66, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTHP_LACLA (Q9CJ83)

Other databases:

- EMBL:   AE005176
- PIR:   E86639
- RefSeq:   NP_266273.1
- ProteinModelPortal:   Q9CJ83
- SMR:   Q9CJ83
- PhosSite:   Q9CJ83
- GeneID:   1113723
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L120335
- NMPDR:   fig|272623.1.peg.124
- HOGENOM:   HBG653254
- OMA:   SEITLEY
- ProtClustDB:   PRK13780
- BioCyc:   LLAC272623:L120335-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9180; Mature: 9048

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKEFHIVVETGIHARPATLLVHTASKFTSEITLEYKGKSVNLKSIMGVMSLGVGQGAD
CCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCEECHHHHHHHHHCCCCCCCC
VTISAEGADADDAISTIAETMTKEGLAE
EEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASKEFHIVVETGIHARPATLLVHTASKFTSEITLEYKGKSVNLKSIMGVMSLGVGQGAD
CCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCEECHHHHHHHHHCCCCCCCC
VTISAEGADADDAISTIAETMTKEGLAE
EEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471