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The map label for this gene is ptsI

Identifier: 15672100

GI number: 15672100

Start: 120649

End: 122376

Strand: Direct

Name: ptsI

Synonym: L120628

Alternate gene names: 15672100

Gene position: 120649-122376 (Clockwise)

Preceding gene: 15672099

Following gene: 15672101

Centisome position: 5.1

GC content: 41.49

Gene sequence:

>1728_bases
ATGACAACTATGCTTAAAGGTATCGCCGCATCATCAGGTGTAGCAGTAGCTAAGGCATACTTGCTTGTCCAACCAGATTT
GTCTTTCGAAACAAAGACAATTGCTGATACAGCTAATGAAGAAGCTCGCCTTGATGCAGCACTTGCTACATCTCAAAGCG
AGCTTCAACTTATTAAGGACAAAGCAGTAACAACCCTTGGTGAAGAAGCAGCATCTGTATTTGACGCTCATATGATGGTC
CTCGCTGACCCAGATATGACTGCTCAAATTAAAGCAGTAATCAACGACAAAAAAGTTAACGCTGAATCAGCCCTCAAAGA
AGTAACTGATATGTTTATCGGTATCTTTGAAGGAATGACTGATAATGCTTATATGCAAGAACGTGCAGCTGACATTAAAG
ACGTTACAAAACGTGTTTTGGCTCACCTTCTTGGTGTTAAATTGCCAAGTCCAGCGCTTATCGATGAAGAAGTAATCATC
GTAGCAGAAGACTTGACACCATCTGATACAGCTCAATTGGACAAGAAATTCGTTAAAGCCTTTGTTACTAACATTGGTGG
ACGTACTTCTCACTCTGCAATTATGGCTCGTACTTTGGAAATTCCTGCTGTTCTTGGAACAAATAATATTACTGAACTTG
TTTCTGAAGGTCAACTTTTGGCTGTTTCAGGATTGACTGGTGAAGTAATTCTTGATCCATCTACTGAACAACAAAGCGAA
TTCCACAAAGCTGGTGAAGCTTATGCTGCTCAAAAAGCAGAATGGGCTGCTCTTAAAGACGCTGAAACTGTTACAGCTGA
TGGACGTCATTATGAGCTTGCTGCTAATATCGGAACACCTAAAGACGTTGAAGGTGTTAACGATAACGGTGCTGAAGCAA
TTGGTCTTTATCGTACAGAATTCTTGTACATGGATGCACAAGATTTCCCTACAGAAGATGACCAATACGAAGCATATAAA
GCTGTACTTGAAGGAATGAACGGTAAACCAGTTGTTGTCCGTACAATGGACATCGGTGGTGACAAAACATTGCCTTACTT
TGACCTTCCTAAAGAAATGAACCCATTCCTCGGATGGCGTGCACTTCGTATCAGCCTTTCAACAGCTGGTGACGGAATGT
TCCGTACACAATTGCGTGCACTCTTGCGTGCTTCTGTACACGGACAACTTCGTATCATGTTCCCAATGGTTGCTCTCGTA
ACTGAGTTCCGTGCAGCTAAGAAGATTTATGACGAAGAAAAAGCAAAATTGATTGCTGAAGGTGTTCCAGTTGCAGATGG
TATCGAAGTAGGTATCATGATTGAAATTCCAGCAGCAGCAATGCTTGCAGACCAATTTGCTAAGGAAGTTGATTTCTTCT
CAATTGGTACAAACGATCTCATCCAATATACAATGGCTGCGGACCGTATGAATGAACAAGTTTCTTACCTTTACCAACCT
TACAATCCATCAATCCTTCGTTTGATTAACAATGTAATCAAAGCAGCTCACGCTGAAGGTAAATGGGCTGGTATGTGTGG
TGAAATGGCCGGCGACCAAACTGCTGTACCATTGCTTATGGGTATGGGGCTTGACGAATTCTCTATGTCAGCAACATCAG
TACTCCAAACTCGTTCACTTATGAAACGTTTGGATTCTAAGAAAATGGAAGAATTGTCTAGCAAAGCTTTGAGCGAATGT
GCAACAATGGAAGAAGTTATTGCCCTCGTTGAAGAATACACTAAATAA

Upstream 100 bases:

>100_bases
AAGGCGCTGACGTTACTATTTCAGCTGAAGGTGCAGATGCTGACGATGCAATCTCAACAATCGCTGAAACAATGACTAAA
GAAGGACTCGCAGAATAATC

Downstream 100 bases:

>100_bases
TCTTTTCAATTGATTTGAATAAATGAATCCTGTCAGTTCTGACAGGATTTTTTGTAATCTATTTATCAACAACAAATTTA
CTGACAAAAAAACTTATCCA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTTMLKGIAASSGVAVAKAYLLVQPDLSFETKTIADTANEEARLDAALATSQSELQLIKDKAVTTLGEEAASVFDAHMMV
LADPDMTAQIKAVINDKKVNAESALKEVTDMFIGIFEGMTDNAYMQERAADIKDVTKRVLAHLLGVKLPSPALIDEEVII
VAEDLTPSDTAQLDKKFVKAFVTNIGGRTSHSAIMARTLEIPAVLGTNNITELVSEGQLLAVSGLTGEVILDPSTEQQSE
FHKAGEAYAAQKAEWAALKDAETVTADGRHYELAANIGTPKDVEGVNDNGAEAIGLYRTEFLYMDAQDFPTEDDQYEAYK
AVLEGMNGKPVVVRTMDIGGDKTLPYFDLPKEMNPFLGWRALRISLSTAGDGMFRTQLRALLRASVHGQLRIMFPMVALV
TEFRAAKKIYDEEKAKLIAEGVPVADGIEVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQTAVPLLMGMGLDEFSMSATSVLQTRSLMKRLDSKKMEELSSKALSEC
ATMEEVIALVEEYTK

Sequences:

>Translated_575_residues
MTTMLKGIAASSGVAVAKAYLLVQPDLSFETKTIADTANEEARLDAALATSQSELQLIKDKAVTTLGEEAASVFDAHMMV
LADPDMTAQIKAVINDKKVNAESALKEVTDMFIGIFEGMTDNAYMQERAADIKDVTKRVLAHLLGVKLPSPALIDEEVII
VAEDLTPSDTAQLDKKFVKAFVTNIGGRTSHSAIMARTLEIPAVLGTNNITELVSEGQLLAVSGLTGEVILDPSTEQQSE
FHKAGEAYAAQKAEWAALKDAETVTADGRHYELAANIGTPKDVEGVNDNGAEAIGLYRTEFLYMDAQDFPTEDDQYEAYK
AVLEGMNGKPVVVRTMDIGGDKTLPYFDLPKEMNPFLGWRALRISLSTAGDGMFRTQLRALLRASVHGQLRIMFPMVALV
TEFRAAKKIYDEEKAKLIAEGVPVADGIEVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQTAVPLLMGMGLDEFSMSATSVLQTRSLMKRLDSKKMEELSSKALSEC
ATMEEVIALVEEYTK
>Mature_574_residues
TTMLKGIAASSGVAVAKAYLLVQPDLSFETKTIADTANEEARLDAALATSQSELQLIKDKAVTTLGEEAASVFDAHMMVL
ADPDMTAQIKAVINDKKVNAESALKEVTDMFIGIFEGMTDNAYMQERAADIKDVTKRVLAHLLGVKLPSPALIDEEVIIV
AEDLTPSDTAQLDKKFVKAFVTNIGGRTSHSAIMARTLEIPAVLGTNNITELVSEGQLLAVSGLTGEVILDPSTEQQSEF
HKAGEAYAAQKAEWAALKDAETVTADGRHYELAANIGTPKDVEGVNDNGAEAIGLYRTEFLYMDAQDFPTEDDQYEAYKA
VLEGMNGKPVVVRTMDIGGDKTLPYFDLPKEMNPFLGWRALRISLSTAGDGMFRTQLRALLRASVHGQLRIMFPMVALVT
EFRAAKKIYDEEKAKLIAEGVPVADGIEVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPY
NPSILRLINNVIKAAHAEGKWAGMCGEMAGDQTAVPLLMGMGLDEFSMSATSVLQTRSLMKRLDSKKMEELSSKALSECA
TMEEVIALVEEYTK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=572, Percent_Identity=48.2517482517483, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1788726, Length=574, Percent_Identity=36.2369337979094, Blast_Score=344, Evalue=9e-96,
Organism=Escherichia coli, GI48994992, Length=504, Percent_Identity=36.7063492063492, Blast_Score=341, Evalue=8e-95,
Organism=Escherichia coli, GI1789193, Length=566, Percent_Identity=33.0388692579505, Blast_Score=290, Evalue=3e-79,
Organism=Escherichia coli, GI1787994, Length=420, Percent_Identity=29.0476190476191, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI226510935, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_LACLA (Q9CJ82)

Other databases:

- EMBL:   AE005176
- PIR:   F86639
- RefSeq:   NP_266274.1
- ProteinModelPortal:   Q9CJ82
- SMR:   Q9CJ82
- GeneID:   1113724
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L120628
- NMPDR:   fig|272623.1.peg.125
- HOGENOM:   HBG456539
- OMA:   MVEIPST
- ProtClustDB:   CLSK876691
- BioCyc:   LLAC272623:L120628-MONOMER
- BRENDA:   2.7.3.9
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 62561; Mature: 62430

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 191-191 ACT_SITE 506-506 BINDING 298-298 BINDING 334-334 BINDING 435-435 BINDING 456-456 BINDING 457-457 BINDING 458-458 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMLKGIAASSGVAVAKAYLLVQPDLSFETKTIADTANEEARLDAALATSQSELQLIKD
CCCHHHHHHCCCCHHEEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
KAVTTLGEEAASVFDAHMMVLADPDMTAQIKAVINDKKVNAESALKEVTDMFIGIFEGMT
HHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
DNAYMQERAADIKDVTKRVLAHLLGVKLPSPALIDEEVIIVAEDLTPSDTAQLDKKFVKA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIPAVLGTNNITELVSEGQLLAVSGLTGEVILDPSTEQQSE
HHHHCCCCCCCHHHHHHHHCCCEEECCCHHHHHHCCCCEEEEECCCCEEEECCCCHHHHH
FHKAGEAYAAQKAEWAALKDAETVTADGRHYELAANIGTPKDVEGVNDNGAEAIGLYRTE
HHHHHHHHHHHCCHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDAQDFPTEDDQYEAYKAVLEGMNGKPVVVRTMDIGGDKTLPYFDLPKEMNPFLGWR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
ALRISLSTAGDGMFRTQLRALLRASVHGQLRIMFPMVALVTEFRAAKKIYDEEKAKLIAE
EEEEEEECCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVPVADGIEVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQTAVPLLMGMGLDEFSMSATSVLQTRSL
CCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
MKRLDSKKMEELSSKALSECATMEEVIALVEEYTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTMLKGIAASSGVAVAKAYLLVQPDLSFETKTIADTANEEARLDAALATSQSELQLIKD
CCHHHHHHCCCCHHEEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
KAVTTLGEEAASVFDAHMMVLADPDMTAQIKAVINDKKVNAESALKEVTDMFIGIFEGMT
HHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
DNAYMQERAADIKDVTKRVLAHLLGVKLPSPALIDEEVIIVAEDLTPSDTAQLDKKFVKA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIPAVLGTNNITELVSEGQLLAVSGLTGEVILDPSTEQQSE
HHHHCCCCCCCHHHHHHHHCCCEEECCCHHHHHHCCCCEEEEECCCCEEEECCCCHHHHH
FHKAGEAYAAQKAEWAALKDAETVTADGRHYELAANIGTPKDVEGVNDNGAEAIGLYRTE
HHHHHHHHHHHCCHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDAQDFPTEDDQYEAYKAVLEGMNGKPVVVRTMDIGGDKTLPYFDLPKEMNPFLGWR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
ALRISLSTAGDGMFRTQLRALLRASVHGQLRIMFPMVALVTEFRAAKKIYDEEKAKLIAE
EEEEEEECCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVPVADGIEVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQTAVPLLMGMGLDEFSMSATSVLQTRSL
CCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
MKRLDSKKMEELSSKALSECATMEEVIALVEEYTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471