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The map label for this gene is livH

Identifier: 15669455

GI number: 15669455

Start: 1213421

End: 1214368

Strand: Direct

Name: livH

Synonym: MJ1269

Alternate gene names: 15669455

Gene position: 1213421-1214368 (Clockwise)

Preceding gene: 15669454

Following gene: 15669456

Centisome position: 72.88

GC content: 31.96

Gene sequence:

>948_bases
TTGGTCAAGCTTTTACTAAAAGATTTAGGTGAGAAAATGATTTTAGAAGGAGCTATTATATACTCCAACCTTTTGGTTTT
GTTGGCTTTGGGATTAACTCTAACTTATATAACAACAAACGTTCCAAACTTTGCTCAGGGAAGTTATGCGATAGTTGGGA
GTTATGTTGCTTTAACATTATTAAAGTTGTTTGGTATTTGTCCTTATCTTTCTTTACCAGTTTTATTTGTTGTTGGGGCA
ATTGTTGGTTTAATAACTTATTTAGCTTTAAAGCCCTTGATAAAAAGAAATGCCTCTGTAGAGATTTTAATGATTGCAAC
TCTTGCTATAGATTTAATATTATTGGGTGTTATTGGGGCTTACTCTGAAATATTAAGTCAAATTGTTGGTTCTACTCAAG
CAAAATTCGTTTTTGCAAACTTGGATTTTTCATTATTTGGATTTAAAGGAATTTTATTTGTTTCAACCTTCGTAGTTATA
CTACTATTAATTGGACTTTATCTCCTCCTGTATAAAACAAAGTTTGGTATTGCTTTGAGAGCTTCAATGGAGAATCCTTC
ATTAGCTCAAACTATGGGAATTGATGTTGAAAAAACAAGATTATTTTCTTGGATTCTTTCTGGAGCTTTAGCTGGAGTTG
CTGGTGGGCTTTTACCTTTCATGCAAGAGATTGTTCCAGCCACTGGGGATTTAATTATTATTTCAATCTTTGCGGCAAGT
ATTGTTGGAGGTTTAAGGCATATAAGTGGAGCATTAATAGGGGGCTATATAATTGGAATATCTGAGAGTTTAATAACTTA
TTATTTAGCATCAGCATTTGGAACAGGATTTTTGGTTTATGGAAAGGTTATCTCCTTAATAATAATGATAGCAACATTAT
TAATAGCACCTTATGGAATTACTGGAGTCGATTGGAAAAAGTTAAAAAGATTATTATCTACTTCATAA

Upstream 100 bases:

>100_bases
GAACAGCAGAAGAGTTGTTAAACCATGAGAAATTTAAGGAATATTCATTAGGAATAACTGCTGTATAAGGTATAATTTTT
ATAAAACCTAATGAACACAT

Downstream 100 bases:

>100_bases
TTTAACTTATGGGGTGAAAATATGAGTATTGATTTAATATCCATGATTTTATTGTGGTTTGGGCTTTATTATATTGTTTC
TCTTTCCTTAAATATGGAAT

Product: high-affinity branched-chain amino acid transport protein BraD

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MVKLLLKDLGEKMILEGAIIYSNLLVLLALGLTLTYITTNVPNFAQGSYAIVGSYVALTLLKLFGICPYLSLPVLFVVGA
IVGLITYLALKPLIKRNASVEILMIATLAIDLILLGVIGAYSEILSQIVGSTQAKFVFANLDFSLFGFKGILFVSTFVVI
LLLIGLYLLLYKTKFGIALRASMENPSLAQTMGIDVEKTRLFSWILSGALAGVAGGLLPFMQEIVPATGDLIIISIFAAS
IVGGLRHISGALIGGYIIGISESLITYYLASAFGTGFLVYGKVISLIIMIATLLIAPYGITGVDWKKLKRLLSTS

Sequences:

>Translated_315_residues
MVKLLLKDLGEKMILEGAIIYSNLLVLLALGLTLTYITTNVPNFAQGSYAIVGSYVALTLLKLFGICPYLSLPVLFVVGA
IVGLITYLALKPLIKRNASVEILMIATLAIDLILLGVIGAYSEILSQIVGSTQAKFVFANLDFSLFGFKGILFVSTFVVI
LLLIGLYLLLYKTKFGIALRASMENPSLAQTMGIDVEKTRLFSWILSGALAGVAGGLLPFMQEIVPATGDLIIISIFAAS
IVGGLRHISGALIGGYIIGISESLITYYLASAFGTGFLVYGKVISLIIMIATLLIAPYGITGVDWKKLKRLLSTS
>Mature_315_residues
MVKLLLKDLGEKMILEGAIIYSNLLVLLALGLTLTYITTNVPNFAQGSYAIVGSYVALTLLKLFGICPYLSLPVLFVVGA
IVGLITYLALKPLIKRNASVEILMIATLAIDLILLGVIGAYSEILSQIVGSTQAKFVFANLDFSLFGFKGILFVSTFVVI
LLLIGLYLLLYKTKFGIALRASMENPSLAQTMGIDVEKTRLFSWILSGALAGVAGGLLPFMQEIVPATGDLIIISIFAAS
IVGGLRHISGALIGGYIIGISESLITYYLASAFGTGFLVYGKVISLIIMIATLLIAPYGITGVDWKKLKRLLSTS

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily

Homologues:

Organism=Escherichia coli, GI1789866, Length=290, Percent_Identity=28.9655172413793, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIVH_METJA (Q58665)

Other databases:

- EMBL:   L77117
- PIR:   D64458
- RefSeq:   NP_248265.1
- ProteinModelPortal:   Q58665
- GeneID:   1452167
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1269
- NMPDR:   fig|243232.1.peg.1302
- TIGR:   MJ1269
- HOGENOM:   HBG728273
- OMA:   LTLTYIT
- ProtClustDB:   CLSK2298724
- BioCyc:   MJAN243232:MJ_1269-MONOMER
- InterPro:   IPR001851

Pfam domain/function: PF02653 BPD_transp_2

EC number: NA

Molecular weight: Translated: 33655; Mature: 33655

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00251 TNF_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xea2f2a0)-; HASH(0xe4be3f8)-; HASH(0xd204404)-; HASH(0xd1583b0)-; HASH(0xe43c9d4)-; HASH(0xe413ff8)-; HASH(0xe23b410)-; HASH(0xb9fa9cc)-; HASH(0xe2790b4)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLLLKDLGEKMILEGAIIYSNLLVLLALGLTLTYITTNVPNFAQGSYAIVGSYVALTL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LKLFGICPYLSLPVLFVVGAIVGLITYLALKPLIKRNASVEILMIATLAIDLILLGVIGA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
YSEILSQIVGSTQAKFVFANLDFSLFGFKGILFVSTFVVILLLIGLYLLLYKTKFGIALR
HHHHHHHHHCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
ASMENPSLAQTMGIDVEKTRLFSWILSGALAGVAGGLLPFMQEIVPATGDLIIISIFAAS
ECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IVGGLRHISGALIGGYIIGISESLITYYLASAFGTGFLVYGKVISLIIMIATLLIAPYGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
TGVDWKKLKRLLSTS
CCCCHHHHHHHHCCH
>Mature Secondary Structure
MVKLLLKDLGEKMILEGAIIYSNLLVLLALGLTLTYITTNVPNFAQGSYAIVGSYVALTL
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LKLFGICPYLSLPVLFVVGAIVGLITYLALKPLIKRNASVEILMIATLAIDLILLGVIGA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
YSEILSQIVGSTQAKFVFANLDFSLFGFKGILFVSTFVVILLLIGLYLLLYKTKFGIALR
HHHHHHHHHCCCHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
ASMENPSLAQTMGIDVEKTRLFSWILSGALAGVAGGLLPFMQEIVPATGDLIIISIFAAS
ECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IVGGLRHISGALIGGYIIGISESLITYYLASAFGTGFLVYGKVISLIIMIATLLIAPYGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
TGVDWKKLKRLLSTS
CCCCHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087