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The map label for this gene is livF [H]

Identifier: 15669454

GI number: 15669454

Start: 1212813

End: 1213388

Strand: Direct

Name: livF [H]

Synonym: MJ1268

Alternate gene names: 15669454

Gene position: 1212813-1213388 (Clockwise)

Preceding gene: 15669453

Following gene: 15669455

Centisome position: 72.84

GC content: 34.72

Gene sequence:

>576_bases
TTGTTTGGTTTAACAAAAATATATTCTGGAGAGATTATATTTAAGGATAAAGATATAGCAAAAGTCCCTCCACATCAAAA
AGCAAGGATGAAAATAGCTTATTTACCACAAACAAACAATGTATTTGCTAATTTGACCGTAGAGGAGAACTTAAAAATTG
CTGGTTATGTATTAGATAAAGATAAGGTAAAGGAAAGAATTGAAATAGCCTTAAGTGTATTCCCAGAGCTTAAGGACATT
TTAAAGAGAAAGGCAGGAACGCTTAGTGGAGGACAGAGGCAGTTCTTAGCCATGGGTATGGCTTTAGTTAGGGATGCTGA
GGTCTTAATGTTAGATGAACCAACTGCTCAATTGTCACCAAAACTCGCTGAAGTGATATTTGAAAAGATTATTGAGATGA
GGGATAACTTTGGACTAACAATTTTGTTGGTTGAGCAAAATGCTAAGAGAGCTTTAGAGATTAGTGATAACGGCTATATG
TTTGTAAGTGGAAGAGTAGCATTTGAGGGAACAGCAGAAGAGTTGTTAAACCATGAGAAATTTAAGGAATATTCATTAGG
AATAACTGCTGTATAA

Upstream 100 bases:

>100_bases
AATTGCAGATACTATTTGATGTGAATGCAAAAATAGAAAAGGGAAAATTACCACAGTTGTTGGACCTAATGGTAGTGGAA
AATCCACATTTTTAAAAACG

Downstream 100 bases:

>100_bases
GGTATAATTTTTATAAAACCTAATGAACACATTTGGTCAAGCTTTTACTAAAAGATTTAGGTGAGAAAATGATTTTAGAA
GGAGCTATTATATACTCCAA

Product: high-affinity branched-chain amino acid transport ATP-binding protein BraG

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MFGLTKIYSGEIIFKDKDIAKVPPHQKARMKIAYLPQTNNVFANLTVEENLKIAGYVLDKDKVKERIEIALSVFPELKDI
LKRKAGTLSGGQRQFLAMGMALVRDAEVLMLDEPTAQLSPKLAEVIFEKIIEMRDNFGLTILLVEQNAKRALEISDNGYM
FVSGRVAFEGTAEELLNHEKFKEYSLGITAV

Sequences:

>Translated_191_residues
MFGLTKIYSGEIIFKDKDIAKVPPHQKARMKIAYLPQTNNVFANLTVEENLKIAGYVLDKDKVKERIEIALSVFPELKDI
LKRKAGTLSGGQRQFLAMGMALVRDAEVLMLDEPTAQLSPKLAEVIFEKIIEMRDNFGLTILLVEQNAKRALEISDNGYM
FVSGRVAFEGTAEELLNHEKFKEYSLGITAV
>Mature_191_residues
MFGLTKIYSGEIIFKDKDIAKVPPHQKARMKIAYLPQTNNVFANLTVEENLKIAGYVLDKDKVKERIEIALSVFPELKDI
LKRKAGTLSGGQRQFLAMGMALVRDAEVLMLDEPTAQLSPKLAEVIFEKIIEMRDNFGLTILLVEQNAKRALEISDNGYM
FVSGRVAFEGTAEELLNHEKFKEYSLGITAV

Specific function: Probable component of a branched-chain amino-acid transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI87082268, Length=187, Percent_Identity=39.0374331550802, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI1786253, Length=183, Percent_Identity=30.0546448087432, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789593, Length=168, Percent_Identity=30.3571428571429, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082267, Length=169, Percent_Identity=32.5443786982249, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789864, Length=203, Percent_Identity=30.0492610837438, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790467, Length=180, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI87081791, Length=181, Percent_Identity=27.6243093922652, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787370, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1787712, Length=166, Percent_Identity=28.9156626506024, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786398, Length=157, Percent_Identity=32.484076433121, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790544, Length=176, Percent_Identity=26.1363636363636, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788450, Length=171, Percent_Identity=25.1461988304094, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87081709, Length=177, Percent_Identity=27.683615819209, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787792, Length=175, Percent_Identity=27.4285714285714, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1786345, Length=183, Percent_Identity=27.3224043715847, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789672, Length=167, Percent_Identity=23.9520958083832, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI48994943, Length=177, Percent_Identity=29.9435028248588, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=175, Percent_Identity=30.8571428571429, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=175, Percent_Identity=30.8571428571429, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=175, Percent_Identity=30.8571428571429, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 21466; Mature: 21466

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLTKIYSGEIIFKDKDIAKVPPHQKARMKIAYLPQTNNVFANLTVEENLKIAGYVLDK
CCCCCEEECCEEEEECCCCCCCCCCCHHEEEEEEECCCCCEEEEEEEECCCEEEEEEECH
DKVKERIEIALSVFPELKDILKRKAGTLSGGQRQFLAMGMALVRDAEVLMLDEPTAQLSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHCH
KLAEVIFEKIIEMRDNFGLTILLVEQNAKRALEISDNGYMFVSGRVAFEGTAEELLNHEK
HHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEECEEEECCCHHHHHCHHH
FKEYSLGITAV
HHHHCCCEECC
>Mature Secondary Structure
MFGLTKIYSGEIIFKDKDIAKVPPHQKARMKIAYLPQTNNVFANLTVEENLKIAGYVLDK
CCCCCEEECCEEEEECCCCCCCCCCCHHEEEEEEECCCCCEEEEEEEECCCEEEEEEECH
DKVKERIEIALSVFPELKDILKRKAGTLSGGQRQFLAMGMALVRDAEVLMLDEPTAQLSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHCH
KLAEVIFEKIIEMRDNFGLTILLVEQNAKRALEISDNGYMFVSGRVAFEGTAEELLNHEK
HHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEECEEEECCCHHHHHCHHH
FKEYSLGITAV
HHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]