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The map label for this gene is pyrG

Identifier: 15669363

GI number: 15669363

Start: 1112076

End: 1113698

Strand: Reverse

Name: pyrG

Synonym: MJ1174

Alternate gene names: 15669363

Gene position: 1113698-1112076 (Counterclockwise)

Preceding gene: 15669364

Following gene: 15669353

Centisome position: 66.89

GC content: 35.06

Gene sequence:

>1623_bases
GTGAAAATAATGAAGTTTATATTTATCACTGGAGGAGTTATATCATCATTAGGTAAAGGAATTACAGCAGCTTCGTTAGG
GAGATTATTGAAAGCAAGAGGATTCAAAGTTAATATGATTAAGATAGACCCTTATCTGCAGATAGATGCAGGAACAATGT
CTCCTTATGAGCATGGAGAGGTTTTTGTTACAGAGGATGGTGGAGAGTCAGATTTAGATTTGGGGCATTATGAGAGGTTT
ATTGATGAGAATTTAACCAAAAACAACAACATAACAACAGGAAAGATATATTGGAGTGTCTTAACAAAGGAGAGGAAGGG
AGAGTATTTAGGAAAGACAGTTCAAGTTATCCCTCACATAACAAATGAGATAAAGGATTGGATTAAAAACCTTGGAGAGG
GGTATGATATAACTATCGTTGAAATTGGAGGAACTGTTGGAGATATTGAAAGCTTACCTTTCTTAGAAGCTATAAGGCAG
TTTAAAAAGGATGTGGGTAAAGAAAACGTTTTATACATCCATGTTTCTCTTTTACCTTATATAAGAGCTGCCGGAGAGTT
GAAGACAAAACCTACTCAACATAGTGTTAAAGAGCTAAGAAGCATCGGAATTCAACCAGATATATTAATTTGTAGAACGG
AAATGCCAATAAGTGATAAAATTAGGGAGAAATTAGCCCTATTCTGTGATGTTGATAAAGAGGCGGTTATTGAGGCAAGA
GATGCAAGAACAATATATGAAGTCCCTCTTAATTTAGAAAAAGAAGGTTTAGGGAAATTAGTTACCAAAAAGTTAAATCT
TCCAGATAGAGAACCAGATTTAGACGAATGGAGAAAGTTTGTTGATAGGGTTATAAACCCATTAAATGAAGTAACTATTG
GTATAGTTGGGAAGTATGTTGAGCTAAAAGATGCTTATTTAAGTATTACAGAGGCATTAATCCATGCTGGAGCTAAAAAT
GACACTAAAGTTAATATAAACTGGATACATTCTGAAAGATTAGAAAGTGAAGAATTTGAAGAATTATTAGATAGGTATAG
AGAAGATAATCAATTAGATGGTATCTTAGTTCCAGGAGGATTTGGAGATAGAGGAGTTGAAGGTAAAATAAACGCTATAA
AATATGCAAGAGAAAACGACATTCCTTTCTTAGGTATATGCATGGGAATGCAGTGTGCAGTTATAGAGTTTGCAAGGAAC
GTTTGTGGCTTAGAGGGAGCGAATTCAACAGAGTTTGATGAAAACACTAAGTATCCAGTTGTTGATTTACTGCCAGAGCA
GAAGGAGATTGATGCAAAAGGAGGAACTATGAGATTAGGAGCTTATCCAGCGATATTGATGGAGGGAACTTTAGCTTATA
AGTTGTATGGAAGAAAGGAAGTTTATGAGAGACATAGACATAGGTATGAGGTTAATCCGGAATATCATGAGATATTAGAA
AATCATGGCTTAACAATTTCTGGAAAATCTCCAGATGGAAGATTGGCAGAGTTTATAGAAATCAGCAAAAATAGATACTT
CATAGCAACACAGGCACATCCAGAGTTTAAATCAAGACCTAACAAACCACATCCATTGTTTGATGGGTTAGTAAGGGCTT
CTTTGGGAGAGAAGATTAAATAA

Upstream 100 bases:

>100_bases
TCCGTGTAGCTTATAAAATTTTTATATTTTTTATTTTATTATCTATATTATTATTATTTTGTTTTTATTTATCTTAATTT
GGTTTATTTAAAAGAAATGG

Downstream 100 bases:

>100_bases
ACTTAAGAGTTAATCTTTAAATAGCTTTTTCTTTTTATAAATGTTGTCATGATGGTCAAAATCAACGAAAATAACTGTTT
TATTATTCTCATCAACGGTG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKIMKFIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKIDPYLQIDAGTMSPYEHGEVFVTEDGGESDLDLGHYERF
IDENLTKNNNITTGKIYWSVLTKERKGEYLGKTVQVIPHITNEIKDWIKNLGEGYDITIVEIGGTVGDIESLPFLEAIRQ
FKKDVGKENVLYIHVSLLPYIRAAGELKTKPTQHSVKELRSIGIQPDILICRTEMPISDKIREKLALFCDVDKEAVIEAR
DARTIYEVPLNLEKEGLGKLVTKKLNLPDREPDLDEWRKFVDRVINPLNEVTIGIVGKYVELKDAYLSITEALIHAGAKN
DTKVNINWIHSERLESEEFEELLDRYREDNQLDGILVPGGFGDRGVEGKINAIKYARENDIPFLGICMGMQCAVIEFARN
VCGLEGANSTEFDENTKYPVVDLLPEQKEIDAKGGTMRLGAYPAILMEGTLAYKLYGRKEVYERHRHRYEVNPEYHEILE
NHGLTISGKSPDGRLAEFIEISKNRYFIATQAHPEFKSRPNKPHPLFDGLVRASLGEKIK

Sequences:

>Translated_540_residues
MKIMKFIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKIDPYLQIDAGTMSPYEHGEVFVTEDGGESDLDLGHYERF
IDENLTKNNNITTGKIYWSVLTKERKGEYLGKTVQVIPHITNEIKDWIKNLGEGYDITIVEIGGTVGDIESLPFLEAIRQ
FKKDVGKENVLYIHVSLLPYIRAAGELKTKPTQHSVKELRSIGIQPDILICRTEMPISDKIREKLALFCDVDKEAVIEAR
DARTIYEVPLNLEKEGLGKLVTKKLNLPDREPDLDEWRKFVDRVINPLNEVTIGIVGKYVELKDAYLSITEALIHAGAKN
DTKVNINWIHSERLESEEFEELLDRYREDNQLDGILVPGGFGDRGVEGKINAIKYARENDIPFLGICMGMQCAVIEFARN
VCGLEGANSTEFDENTKYPVVDLLPEQKEIDAKGGTMRLGAYPAILMEGTLAYKLYGRKEVYERHRHRYEVNPEYHEILE
NHGLTISGKSPDGRLAEFIEISKNRYFIATQAHPEFKSRPNKPHPLFDGLVRASLGEKIK
>Mature_540_residues
MKIMKFIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKIDPYLQIDAGTMSPYEHGEVFVTEDGGESDLDLGHYERF
IDENLTKNNNITTGKIYWSVLTKERKGEYLGKTVQVIPHITNEIKDWIKNLGEGYDITIVEIGGTVGDIESLPFLEAIRQ
FKKDVGKENVLYIHVSLLPYIRAAGELKTKPTQHSVKELRSIGIQPDILICRTEMPISDKIREKLALFCDVDKEAVIEAR
DARTIYEVPLNLEKEGLGKLVTKKLNLPDREPDLDEWRKFVDRVINPLNEVTIGIVGKYVELKDAYLSITEALIHAGAKN
DTKVNINWIHSERLESEEFEELLDRYREDNQLDGILVPGGFGDRGVEGKINAIKYARENDIPFLGICMGMQCAVIEFARN
VCGLEGANSTEFDENTKYPVVDLLPEQKEIDAKGGTMRLGAYPAILMEGTLAYKLYGRKEVYERHRHRYEVNPEYHEILE
NHGLTISGKSPDGRLAEFIEISKNRYFIATQAHPEFKSRPNKPHPLFDGLVRASLGEKIK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=550, Percent_Identity=49.8181818181818, Blast_Score=534, Evalue=1e-152,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=48.5454545454545, Blast_Score=517, Evalue=1e-147,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=48.5454545454545, Blast_Score=517, Evalue=1e-147,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=48.5454545454545, Blast_Score=517, Evalue=1e-147,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=54.1743970315399, Blast_Score=577, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=42.2185430463576, Blast_Score=462, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=561, Percent_Identity=47.415329768271, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6322563, Length=566, Percent_Identity=46.9964664310954, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=49.9089253187614, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=47.9757085020243, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_METJA (Q58574)

Other databases:

- EMBL:   L77117
- PIR:   E64446
- RefSeq:   NP_248168.1
- ProteinModelPortal:   Q58574
- SMR:   Q58574
- GeneID:   1452072
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1174
- NMPDR:   fig|243232.1.peg.1205
- TIGR:   MJ1174
- HOGENOM:   HBG597806
- OMA:   NIKIDPY
- ProtClustDB:   PRK05380
- BioCyc:   MJAN243232:MJ_1174-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61024; Mature: 61024

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 387-387 ACT_SITE 513-513 ACT_SITE 515-515

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIMKFIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKIDPYLQIDAGTMSPYEHGE
CCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCCCCCCCE
VFVTEDGGESDLDLGHYERFIDENLTKNNNITTGKIYWSVLTKERKGEYLGKTVQVIPHI
EEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEHEEEEEEHHHHHCCHHHHHHHHHHHHH
TNEIKDWIKNLGEGYDITIVEIGGTVGDIESLPFLEAIRQFKKDVGKENVLYIHVSLLPY
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
IRAAGELKTKPTQHSVKELRSIGIQPDILICRTEMPISDKIREKLALFCDVDKEAVIEAR
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHC
DARTIYEVPLNLEKEGLGKLVTKKLNLPDREPDLDEWRKFVDRVINPLNEVTIGIVGKYV
CCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
ELKDAYLSITEALIHAGAKNDTKVNINWIHSERLESEEFEELLDRYREDNQLDGILVPGG
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCHHHHHHHHHHHCCCCCCCEEEECCC
FGDRGVEGKINAIKYARENDIPFLGICMGMQCAVIEFARNVCGLEGANSTEFDENTKYPV
CCCCCCCCCCCEEEEECCCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
VDLLPEQKEIDAKGGTMRLGAYPAILMEGTLAYKLYGRKEVYERHRHRYEVNPEYHEILE
EECCCCCHHCCCCCCEEEECCCCEEEECCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHH
NHGLTISGKSPDGRLAEFIEISKNRYFIATQAHPEFKSRPNKPHPLFDGLVRASLGEKIK
HCCEEEECCCCCCHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIMKFIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKIDPYLQIDAGTMSPYEHGE
CCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCCCCCCCE
VFVTEDGGESDLDLGHYERFIDENLTKNNNITTGKIYWSVLTKERKGEYLGKTVQVIPHI
EEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEHEEEEEEHHHHHCCHHHHHHHHHHHHH
TNEIKDWIKNLGEGYDITIVEIGGTVGDIESLPFLEAIRQFKKDVGKENVLYIHVSLLPY
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
IRAAGELKTKPTQHSVKELRSIGIQPDILICRTEMPISDKIREKLALFCDVDKEAVIEAR
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHC
DARTIYEVPLNLEKEGLGKLVTKKLNLPDREPDLDEWRKFVDRVINPLNEVTIGIVGKYV
CCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
ELKDAYLSITEALIHAGAKNDTKVNINWIHSERLESEEFEELLDRYREDNQLDGILVPGG
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCHHHHHHHHHHHCCCCCCCEEEECCC
FGDRGVEGKINAIKYARENDIPFLGICMGMQCAVIEFARNVCGLEGANSTEFDENTKYPV
CCCCCCCCCCCEEEEECCCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
VDLLPEQKEIDAKGGTMRLGAYPAILMEGTLAYKLYGRKEVYERHRHRYEVNPEYHEILE
EECCCCCHHCCCCCCEEEECCCCEEEECCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHH
NHGLTISGKSPDGRLAEFIEISKNRYFIATQAHPEFKSRPNKPHPLFDGLVRASLGEKIK
HCCEEEECCCCCCHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087