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The map label for this gene is rpoB1

Identifier: 15669230

GI number: 15669230

Start: 972587

End: 974497

Strand: Direct

Name: rpoB1

Synonym: MJ1041

Alternate gene names: 15669230

Gene position: 972587-974497 (Clockwise)

Preceding gene: 15669229

Following gene: 15669231

Centisome position: 58.41

GC content: 37.47

Gene sequence:

>1911_bases
GTGAAAAACTTGGCATCAAGGGAAGTAAATATTTATGTTAATGGAAAGTTAGTTGGAACTACAGACAAACCAGAAGAACT
TGTTAATTTTATTAGGGAAAAAAGAAGAAAAGGAGAGCTCCCTCAATATACAACAGTTGCTTACAATGAAGAAAGTAATG
ACATTCATATAAACACGGATGCTGGAAGGATTGTTAGACCTTTAATAGTTGTAGAAAATGGAAAACCAAAACTAACTAAA
GAACATATTGAAAAATTAAAGAAAGGAGAAATTACATTCTCAGATTTAGTTAAAGAAGGAGTTATTGAATATTTAGACGC
TGAAGAGGAAGAAAATGCCTATATTGCTCTATCTGAAGAAGAATTGACTGAGAAACACACACACTTAGAGATAGACCCAT
TAACCATATTAGGTATTGGGGCAGGGGTTGCTCCTTATCCAGAACACAACTCAGCACCAAGAATTACAATGGCTGCAGCA
ATGGGGAAGCAGTCATTAGGAATTCCAATGAGCAATATAAAATGGAGATTAGATACAAGAGGGCATTATCTCCACTATCC
ACAAGTTCCAATTGTTAGGACAAAACACCAAGAGATTTTAGGTTTCGATAAAAGACCAGCAGGGCAGAACTTCGTTGTAG
CTATTATGAGCTATGAAGGATACAACATGGAGGATGCTATAGTCTTTAACAAATCAGCAATTGATAGAGGTTTGGGAAGA
AGCACCTTCTTCAGAACTTACGATGCGTGTGAGAGAAGATACCCAGGAGGACAGATGGATAGGTTTGAGATTCCTGATAA
AGGAGTAAGGGGTTATAGGTCAGAGGAGTGTTATAGATACTTAGAAGAGGATGGAATAGTTGCAGTTGAGTCCCATGTAA
AAGGTGGAGATGTAATTGTTGGTAAAACCTCTCCACCAAGATTCTTAGAGGAGCATGAGATAACAATTCAAGTTAAACCA
CAGAGAAGAGATTCATCAGTTGTTGTAAGACATGGGGAAGAAGGTTATATAGATAAAGTTATATTAACTGAAACAAAGGA
AGGAAACAGGTTAGTTAAGGTTAAAGTTAGAGATTTGAGAATTCCAGAGCTTGGAGATAAATTTGCTTCAAGACATGGAC
AGAAAGGAGTTATGGGACTTACAGTTCCACAGGAGGATTTACCTTTCACTGAGAGTGGAATAGTTCCTGATATCATAATC
AACCCACACGCAATTCCATCAAGAATGACTGTTGGGCAGTTATTGGAGATGCTTGGGGGTAAAGTAGGAGCTTTAGAAGG
AAGAAGAATAGACGGAACAATATTTAGCGGAGAAAAAGAATGGGATTTAAGAAAGGCTTTAGAGGCTTTAGGATTCAAGC
ATCACGGTAAAGAGGTCATGTATGATGGAAAAACTGGAAAGAAGTTTGAAGTAGAGATTTACATTGGAATAGCATACTAC
CAGAAATTACACCACTTAGTAGCTGGAAAGATACATGCAAGAAGTAGAGGACCTGTCCAAGTGCTAACAAGACAGCCAAC
AGAAGGTAGGGCAAGAGAGGGAGGTCTAAGGTTTGGAGAGATGGAGAGGGATGTTTTAATTGGGCATGGTGCAGCTATGC
TATTAAAAGAGAGGCTTATGGATGAATCAGACCCATATGATATCTGTATATGTTCAAAATGTGGAGACTTTGCCATATTA
GACTACAAGAGAGGGCTAAAATACTGTCCAATTTGTGGAGAGATTGAAAACCTCTACAGCTCTAAGAAGATACCATTCGT
TAGAATACCTTACGCATTCAAGCTTTTATTAGATGAGTTGAAGAGTATGTGTATCTTGCCAAGAATTAAGGTTAGAGATA
AGGTTGAATTAGAGGATTTTGAGGAGTTTGTTGAAAAATTGGAGAAAGAAAAAGTTAAACAGAAACAATAA

Upstream 100 bases:

>100_bases
ATAAATGTTTCTTAAAAGAAACTTTATTTATTATGTTTTTTCTGAGTTTTTGTTGATGTTTAATTTTAATCTTTTCAAAA
TCAATAAAAATCTCTCTTAG

Downstream 100 bases:

>100_bases
AGAGAAACATTAAAAGACCTTGCTTAACCTTAATCTTGCCTTATTACAATATTTTTAGAAAAATAAAAAGTAAAAATAAA
AGCAAATCCTTAATCTTTGG

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MKNLASREVNIYVNGKLVGTTDKPEELVNFIREKRRKGELPQYTTVAYNEESNDIHINTDAGRIVRPLIVVENGKPKLTK
EHIEKLKKGEITFSDLVKEGVIEYLDAEEEENAYIALSEEELTEKHTHLEIDPLTILGIGAGVAPYPEHNSAPRITMAAA
MGKQSLGIPMSNIKWRLDTRGHYLHYPQVPIVRTKHQEILGFDKRPAGQNFVVAIMSYEGYNMEDAIVFNKSAIDRGLGR
STFFRTYDACERRYPGGQMDRFEIPDKGVRGYRSEECYRYLEEDGIVAVESHVKGGDVIVGKTSPPRFLEEHEITIQVKP
QRRDSSVVVRHGEEGYIDKVILTETKEGNRLVKVKVRDLRIPELGDKFASRHGQKGVMGLTVPQEDLPFTESGIVPDIII
NPHAIPSRMTVGQLLEMLGGKVGALEGRRIDGTIFSGEKEWDLRKALEALGFKHHGKEVMYDGKTGKKFEVEIYIGIAYY
QKLHHLVAGKIHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLMDESDPYDICICSKCGDFAIL
DYKRGLKYCPICGEIENLYSSKKIPFVRIPYAFKLLLDELKSMCILPRIKVRDKVELEDFEEFVEKLEKEKVKQKQ

Sequences:

>Translated_636_residues
MKNLASREVNIYVNGKLVGTTDKPEELVNFIREKRRKGELPQYTTVAYNEESNDIHINTDAGRIVRPLIVVENGKPKLTK
EHIEKLKKGEITFSDLVKEGVIEYLDAEEEENAYIALSEEELTEKHTHLEIDPLTILGIGAGVAPYPEHNSAPRITMAAA
MGKQSLGIPMSNIKWRLDTRGHYLHYPQVPIVRTKHQEILGFDKRPAGQNFVVAIMSYEGYNMEDAIVFNKSAIDRGLGR
STFFRTYDACERRYPGGQMDRFEIPDKGVRGYRSEECYRYLEEDGIVAVESHVKGGDVIVGKTSPPRFLEEHEITIQVKP
QRRDSSVVVRHGEEGYIDKVILTETKEGNRLVKVKVRDLRIPELGDKFASRHGQKGVMGLTVPQEDLPFTESGIVPDIII
NPHAIPSRMTVGQLLEMLGGKVGALEGRRIDGTIFSGEKEWDLRKALEALGFKHHGKEVMYDGKTGKKFEVEIYIGIAYY
QKLHHLVAGKIHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLMDESDPYDICICSKCGDFAIL
DYKRGLKYCPICGEIENLYSSKKIPFVRIPYAFKLLLDELKSMCILPRIKVRDKVELEDFEEFVEKLEKEKVKQKQ
>Mature_636_residues
MKNLASREVNIYVNGKLVGTTDKPEELVNFIREKRRKGELPQYTTVAYNEESNDIHINTDAGRIVRPLIVVENGKPKLTK
EHIEKLKKGEITFSDLVKEGVIEYLDAEEEENAYIALSEEELTEKHTHLEIDPLTILGIGAGVAPYPEHNSAPRITMAAA
MGKQSLGIPMSNIKWRLDTRGHYLHYPQVPIVRTKHQEILGFDKRPAGQNFVVAIMSYEGYNMEDAIVFNKSAIDRGLGR
STFFRTYDACERRYPGGQMDRFEIPDKGVRGYRSEECYRYLEEDGIVAVESHVKGGDVIVGKTSPPRFLEEHEITIQVKP
QRRDSSVVVRHGEEGYIDKVILTETKEGNRLVKVKVRDLRIPELGDKFASRHGQKGVMGLTVPQEDLPFTESGIVPDIII
NPHAIPSRMTVGQLLEMLGGKVGALEGRRIDGTIFSGEKEWDLRKALEALGFKHHGKEVMYDGKTGKKFEVEIYIGIAYY
QKLHHLVAGKIHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLMDESDPYDICICSKCGDFAIL
DYKRGLKYCPICGEIENLYSSKKIPFVRIPYAFKLLLDELKSMCILPRIKVRDKVELEDFEEFVEKLEKEKVKQKQ

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family

Homologues:

Organism=Homo sapiens, GI4505941, Length=612, Percent_Identity=45.7516339869281, Blast_Score=531, Evalue=1e-151,
Organism=Homo sapiens, GI238908503, Length=608, Percent_Identity=45.0657894736842, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI238908505, Length=608, Percent_Identity=45.0657894736842, Blast_Score=529, Evalue=1e-150,
Organism=Homo sapiens, GI212286172, Length=634, Percent_Identity=31.8611987381703, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI33469941, Length=634, Percent_Identity=31.8611987381703, Blast_Score=297, Evalue=3e-80,
Organism=Escherichia coli, GI1790419, Length=249, Percent_Identity=34.136546184739, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17552304, Length=615, Percent_Identity=44.390243902439, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI25144348, Length=610, Percent_Identity=42.7868852459016, Blast_Score=512, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17506623, Length=641, Percent_Identity=29.6411856474259, Blast_Score=281, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6324781, Length=618, Percent_Identity=44.4983818770227, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324725, Length=643, Percent_Identity=43.0793157076205, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6325267, Length=459, Percent_Identity=39.4335511982571, Blast_Score=314, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17136444, Length=618, Percent_Identity=45.1456310679612, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI17647877, Length=605, Percent_Identity=43.6363636363636, Blast_Score=524, Evalue=1e-149,
Organism=Drosophila melanogaster, GI17136446, Length=633, Percent_Identity=31.4375987361769, Blast_Score=297, Evalue=2e-80,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RPOB1_METJA (Q60181)

Other databases:

- EMBL:   L77117
- PIR:   H64429
- RefSeq:   NP_248035.1
- ProteinModelPortal:   Q60181
- GeneID:   1451938
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1041
- NMPDR:   fig|243232.1.peg.1072
- TIGR:   MJ1041
- HOGENOM:   HBG496836
- OMA:   EMIGGKV
- ProtClustDB:   PRK07225
- BioCyc:   MJAN243232:MJ_1041-MONOMER
- BRENDA:   2.7.7.6
- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724
- Gene3D:   G3DSA:2.40.50.150
- PANTHER:   PTHR20856
- TIGRFAMs:   TIGR03670

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7

EC number: =2.7.7.6

Molecular weight: Translated: 72194; Mature: 72194

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLASREVNIYVNGKLVGTTDKPEELVNFIREKRRKGELPQYTTVAYNEESNDIHINTD
CCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECC
AGRIVRPLIVVENGKPKLTKEHIEKLKKGEITFSDLVKEGVIEYLDAEEEENAYIALSEE
CCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECHH
ELTEKHTHLEIDPLTILGIGAGVAPYPEHNSAPRITMAAAMGKQSLGIPMSNIKWRLDTR
HHHHHHCEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHCCCCHHHCEEEEECC
GHYLHYPQVPIVRTKHQEILGFDKRPAGQNFVVAIMSYEGYNMEDAIVFNKSAIDRGLGR
CCEEECCCCCEEECCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCCC
STFFRTYDACERRYPGGQMDRFEIPDKGVRGYRSEECYRYLEEDGIVAVESHVKGGDVIV
HHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEE
GKTSPPRFLEEHEITIQVKPQRRDSSVVVRHGEEGYIDKVILTETKEGNRLVKVKVRDLR
ECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEEEEEEC
IPELGDKFASRHGQKGVMGLTVPQEDLPFTESGIVPDIIINPHAIPSRMTVGQLLEMLGG
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCC
KVGALEGRRIDGTIFSGEKEWDLRKALEALGFKHHGKEVMYDGKTGKKFEVEIYIGIAYY
CEECCCCCEECCEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEHHHH
QKLHHLVAGKIHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLM
HHHHHHHHHHEECCCCCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHHHHHC
DESDPYDICICSKCGDFAILDYKRGLKYCPICGEIENLYSSKKIPFVRIPYAFKLLLDEL
CCCCCCCEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHH
KSMCILPRIKVRDKVELEDFEEFVEKLEKEKVKQKQ
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNLASREVNIYVNGKLVGTTDKPEELVNFIREKRRKGELPQYTTVAYNEESNDIHINTD
CCCCCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECC
AGRIVRPLIVVENGKPKLTKEHIEKLKKGEITFSDLVKEGVIEYLDAEEEENAYIALSEE
CCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECHH
ELTEKHTHLEIDPLTILGIGAGVAPYPEHNSAPRITMAAAMGKQSLGIPMSNIKWRLDTR
HHHHHHCEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHCCCCHHHCEEEEECC
GHYLHYPQVPIVRTKHQEILGFDKRPAGQNFVVAIMSYEGYNMEDAIVFNKSAIDRGLGR
CCEEECCCCCEEECCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCCC
STFFRTYDACERRYPGGQMDRFEIPDKGVRGYRSEECYRYLEEDGIVAVESHVKGGDVIV
HHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEE
GKTSPPRFLEEHEITIQVKPQRRDSSVVVRHGEEGYIDKVILTETKEGNRLVKVKVRDLR
ECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEEEEEEC
IPELGDKFASRHGQKGVMGLTVPQEDLPFTESGIVPDIIINPHAIPSRMTVGQLLEMLGG
CCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCC
KVGALEGRRIDGTIFSGEKEWDLRKALEALGFKHHGKEVMYDGKTGKKFEVEIYIGIAYY
CEECCCCCEECCEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEHHHH
QKLHHLVAGKIHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLM
HHHHHHHHHHEECCCCCCEEEEECCCCCCCCHHCCCCCCCCCCEEEEECCHHHHHHHHHC
DESDPYDICICSKCGDFAILDYKRGLKYCPICGEIENLYSSKKIPFVRIPYAFKLLLDEL
CCCCCCCEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHH
KSMCILPRIKVRDKVELEDFEEFVEKLEKEKVKQKQ
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087