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The map label for this gene is rpoB2

Identifier: 15669229

GI number: 15669229

Start: 971005

End: 972501

Strand: Direct

Name: rpoB2

Synonym: MJ1040

Alternate gene names: 15669229

Gene position: 971005-972501 (Clockwise)

Preceding gene: 15669228

Following gene: 15669230

Centisome position: 58.32

GC content: 35.34

Gene sequence:

>1497_bases
GTGGCAATTATGAGAGAATTAGTAGATGCTTATTTTAAAGAACATGGTTTAATAGACCACCAGATAGAGTCATACAACGA
TTTTGTAGAAAATAGATTACAAAAAATTATTGATGAAGTTGGATATATTGAAACAGAGATTACTGGAGGGTATAAAGTTA
AATTAGGGAAAATTAAAGTAGGAAAACCAGTTATTAAAGAAGCTGATGGTTCAATAAGACCAATAACTCCAATGGAAGCA
AGAATCAGAGATTTAACATATTCAGTTCCATTATATTTAGAAATGACCCCTATTATTGGTGAAGGAGAAGATGCAAGGGA
AGGGGAAACTGTTGAAGTTTATATCGGAGAACTTCCAGTAATGCTTGGTTCAAAAATCTGCCACTTGTATGGAAAATCAA
GAGAAGAGCTTATAGACCTGGGAGAAGACCCTGAAGACCCATTTGGCTATTTTATAATTAATGGAACTGAAAAAGTATTA
ATTACTCAGGAAGATTTAATTCCAAATAGAATTTTATGTGAGAAGGCAGAGAGAAGTGGAAAGATAGTAGATGTTGCAAA
AGTGTTTTCAACAAGACACGGATTTAGGGCTTTATGTACTGTTGAAAGACATCCAGATGGTTTGTTGTATGCTACATTCC
CTGGAATGCCTGGGCAGATACCATTGGTTATATTAATGAAAGCACTTGGGGCTGAGACAGATAAAGATATCATTGAGTCA
ATTGATGATGAAAGATTCTTTATGGAGATTGTTTTAAACATTCAAGAGATTAGGGAAGAGCATAACATAAACTCCCCTGA
AGATGCTTTGGAGTTTATTGGAAAGAGAGTAGCTCCAGGGCAGGCAAAGGATTATAGATTAAAGAGAGCAGAAACAGTTT
TATGTAACTATTTACTGCCCCATTTAGGAGTTACAAAAGAAGATTTCCCAAAGAAAATTAGGTTCTTAGGAATAATGGCA
AGAAACGCTTTAGAACTTTACTTTGGATATAGAGGAGAAGATGATAAAGACCACTACGCATATAAAAGAGCTAAATTAGC
TGGGGACTTAATGGAAGATTTGTTTAGATATGCGTTTAGCCAACTTGTTAAGGATATAAAATATCAATTAGAGAGACAAA
CATTAAGGAACAAAACTCCTTCAATTCAAGCTGCTGTAAGGAGCGATATATTAACCGAGAGAATTAAACACGCTATGGCA
ACAGGAACATGGGTTGGAGGAAAAACTGGGGTTAGCCAGCTCTTAGATAGAACAAGTTACTTAGCAACAAACTCTCAATT
GAGGAGAATAGTTTCACCATTATCAAGGTCACAGCCACACTTTGAAGCGAGAGAACTGCATGGAACTCATTGGGGTAAAA
TCTGCCCTTCAGAAACTCCAGAAGGTCCAAACTGTGGTCTTGTTAAAAACTTTGCTATAATGTGTAAAGTTACAAGAGAA
GAAGATGATAGCAAAGTTATTGAGCTATTAAAATCCTTTGGAATAAATGTTTCTTAA

Upstream 100 bases:

>100_bases
GCTAAAGAAGGAGATGTTGTTAGAGTAATTAGAAAAAGTCCAACGGCTGGAGTTAGTATAGCTTATAGATTAGTTATTAA
AAGGATTATTTAAAAAATAG

Downstream 100 bases:

>100_bases
AAGAAACTTTATTTATTATGTTTTTTCTGAGTTTTTGTTGATGTTTAATTTTAATCTTTTCAAAATCAATAAAAATCTCT
CTTAGGTGAAAAACTTGGCA

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAIMRELVDAYFKEHGLIDHQIESYNDFVENRLQKIIDEVGYIETEITGGYKVKLGKIKVGKPVIKEADGSIRPITPMEA
RIRDLTYSVPLYLEMTPIIGEGEDAREGETVEVYIGELPVMLGSKICHLYGKSREELIDLGEDPEDPFGYFIINGTEKVL
ITQEDLIPNRILCEKAERSGKIVDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGQIPLVILMKALGAETDKDIIES
IDDERFFMEIVLNIQEIREEHNINSPEDALEFIGKRVAPGQAKDYRLKRAETVLCNYLLPHLGVTKEDFPKKIRFLGIMA
RNALELYFGYRGEDDKDHYAYKRAKLAGDLMEDLFRYAFSQLVKDIKYQLERQTLRNKTPSIQAAVRSDILTERIKHAMA
TGTWVGGKTGVSQLLDRTSYLATNSQLRRIVSPLSRSQPHFEARELHGTHWGKICPSETPEGPNCGLVKNFAIMCKVTRE
EDDSKVIELLKSFGINVS

Sequences:

>Translated_498_residues
MAIMRELVDAYFKEHGLIDHQIESYNDFVENRLQKIIDEVGYIETEITGGYKVKLGKIKVGKPVIKEADGSIRPITPMEA
RIRDLTYSVPLYLEMTPIIGEGEDAREGETVEVYIGELPVMLGSKICHLYGKSREELIDLGEDPEDPFGYFIINGTEKVL
ITQEDLIPNRILCEKAERSGKIVDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGQIPLVILMKALGAETDKDIIES
IDDERFFMEIVLNIQEIREEHNINSPEDALEFIGKRVAPGQAKDYRLKRAETVLCNYLLPHLGVTKEDFPKKIRFLGIMA
RNALELYFGYRGEDDKDHYAYKRAKLAGDLMEDLFRYAFSQLVKDIKYQLERQTLRNKTPSIQAAVRSDILTERIKHAMA
TGTWVGGKTGVSQLLDRTSYLATNSQLRRIVSPLSRSQPHFEARELHGTHWGKICPSETPEGPNCGLVKNFAIMCKVTRE
EDDSKVIELLKSFGINVS
>Mature_497_residues
AIMRELVDAYFKEHGLIDHQIESYNDFVENRLQKIIDEVGYIETEITGGYKVKLGKIKVGKPVIKEADGSIRPITPMEAR
IRDLTYSVPLYLEMTPIIGEGEDAREGETVEVYIGELPVMLGSKICHLYGKSREELIDLGEDPEDPFGYFIINGTEKVLI
TQEDLIPNRILCEKAERSGKIVDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGQIPLVILMKALGAETDKDIIESI
DDERFFMEIVLNIQEIREEHNINSPEDALEFIGKRVAPGQAKDYRLKRAETVLCNYLLPHLGVTKEDFPKKIRFLGIMAR
NALELYFGYRGEDDKDHYAYKRAKLAGDLMEDLFRYAFSQLVKDIKYQLERQTLRNKTPSIQAAVRSDILTERIKHAMAT
GTWVGGKTGVSQLLDRTSYLATNSQLRRIVSPLSRSQPHFEARELHGTHWGKICPSETPEGPNCGLVKNFAIMCKVTREE
DDSKVIELLKSFGINVS

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family

Homologues:

Organism=Homo sapiens, GI4505941, Length=534, Percent_Identity=35.3932584269663, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI238908503, Length=503, Percent_Identity=32.0079522862823, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI238908505, Length=459, Percent_Identity=32.6797385620915, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI33469941, Length=476, Percent_Identity=24.1596638655462, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI212286172, Length=428, Percent_Identity=24.2990654205607, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1790419, Length=153, Percent_Identity=29.4117647058824, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17552304, Length=533, Percent_Identity=33.5834896810507, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25144348, Length=514, Percent_Identity=29.3774319066148, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17506623, Length=458, Percent_Identity=26.2008733624454, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324725, Length=533, Percent_Identity=34.7091932457786, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6324781, Length=502, Percent_Identity=30.2788844621514, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325267, Length=500, Percent_Identity=25, Blast_Score=106, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17136444, Length=528, Percent_Identity=35.9848484848485, Blast_Score=304, Evalue=7e-83,
Organism=Drosophila melanogaster, GI17647877, Length=514, Percent_Identity=28.7937743190661, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RPOB2_METJA (Q58444)

Other databases:

- EMBL:   L77117
- PIR:   G64429
- RefSeq:   NP_248034.1
- ProteinModelPortal:   Q58444
- SMR:   Q58444
- GeneID:   1451937
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1040
- NMPDR:   fig|243232.1.peg.1071
- TIGR:   MJ1040
- HOGENOM:   HBG496841
- OMA:   LAGDLME
- ProtClustDB:   PRK09606
- BioCyc:   MJAN243232:MJ_1040-MONOMER
- BRENDA:   2.7.7.6
- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645
- PANTHER:   PTHR20856

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3

EC number: =2.7.7.6

Molecular weight: Translated: 56488; Mature: 56357

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIMRELVDAYFKEHGLIDHQIESYNDFVENRLQKIIDEVGYIETEITGGYKVKLGKIKV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEEEC
GKPVIKEADGSIRPITPMEARIRDLTYSVPLYLEMTPIIGEGEDAREGETVEVYIGELPV
CCCHHHHCCCCCCCCCCHHHHHHHHEEECEEEEEECCCCCCCCCCCCCCEEEEEECCCCH
MLGSKICHLYGKSREELIDLGEDPEDPFGYFIINGTEKVLITQEDLIPNRILCEKAERSG
HHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEECCCCEEEEEHHHCCCCHHHHHHHHCCC
KIVDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGQIPLVILMKALGAETDKDIIES
CEEHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHH
IDDERFFMEIVLNIQEIREEHNINSPEDALEFIGKRVAPGQAKDYRLKRAETVLCNYLLP
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HLGVTKEDFPKKIRFLGIMARNALELYFGYRGEDDKDHYAYKRAKLAGDLMEDLFRYAFS
HCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLVKDIKYQLERQTLRNKTPSIQAAVRSDILTERIKHAMATGTWVGGKTGVSQLLDRTSY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LATNSQLRRIVSPLSRSQPHFEARELHGTHWGKICPSETPEGPNCGLVKNFAIMCKVTRE
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHCEEEEEEEECC
EDDSKVIELLKSFGINVS
CCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AIMRELVDAYFKEHGLIDHQIESYNDFVENRLQKIIDEVGYIETEITGGYKVKLGKIKV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEEEEC
GKPVIKEADGSIRPITPMEARIRDLTYSVPLYLEMTPIIGEGEDAREGETVEVYIGELPV
CCCHHHHCCCCCCCCCCHHHHHHHHEEECEEEEEECCCCCCCCCCCCCCEEEEEECCCCH
MLGSKICHLYGKSREELIDLGEDPEDPFGYFIINGTEKVLITQEDLIPNRILCEKAERSG
HHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEECCCCEEEEEHHHCCCCHHHHHHHHCCC
KIVDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGQIPLVILMKALGAETDKDIIES
CEEHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHH
IDDERFFMEIVLNIQEIREEHNINSPEDALEFIGKRVAPGQAKDYRLKRAETVLCNYLLP
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HLGVTKEDFPKKIRFLGIMARNALELYFGYRGEDDKDHYAYKRAKLAGDLMEDLFRYAFS
HCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLVKDIKYQLERQTLRNKTPSIQAAVRSDILTERIKHAMATGTWVGGKTGVSQLLDRTSY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LATNSQLRRIVSPLSRSQPHFEARELHGTHWGKICPSETPEGPNCGLVKNFAIMCKVTRE
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHCEEEEEEEECC
EDDSKVIELLKSFGINVS
CCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087