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The map label for this gene is dapA

Identifier: 15668419

GI number: 15668419

Start: 232579

End: 233448

Strand: Reverse

Name: dapA

Synonym: MJ0244

Alternate gene names: 15668419

Gene position: 233448-232579 (Counterclockwise)

Preceding gene: 15668420

Following gene: 15668418

Centisome position: 14.02

GC content: 36.44

Gene sequence:

>870_bases
ATGTTTAAGGGGGTCTATCCCGCCATCATTACTCCTTTTAAAAATAAAGAAGTTGATTTTGATGGATTAGAGGAGAACAT
AAACTTTTTAATTGAAAATGGAGTTAGCGGAATTGTAGCTGTTGGAACTACTGGAGAAAGCCCTACTCTATCCCACGAAG
AGCATAAAAAAGTTATTGAAAAAGTGGTAGATGTTGTTAATGGGCGAGTTCAGGTTATTGCAGGAGCTGGGTCAAACTGC
ACAGAGGAGGCAATAGAGCTTTCTGTTTTTGCTGAAGATGTGGGAGCAGATGCTGTATTGTCAATAACTCCCTATTATAA
TAAACCAACACAGGAAGGTTTAAGAAAGCATTTTGGAAAGGTTGCTGAATCTATAAATCTTCCAATAGTTTTATATAATG
TTCCATCAAGAACAGCTGTTAATTTAGAACCAAAGACAGTAAAGCTTTTAGCTGAAGAATACAGCAATATTTCAGCAGTT
AAAGAGGCAAATCCAAATCTTTCCCAAGTTTCTGAGCTAATACATGATGCTAAGATAACAGTTCTTTCAGGGAATGATGA
ACTAACCCTCCCAATAATCGCCTTAGGAGGAAAAGGGGTTATTAGCGTAGTGGCCAACATCGTCCCAAAAGAGTTTGTTG
AAATGGTTAATTACGCATTAGAAGGAGATTTCGAAAAAGCAAGGGAAATTCATTATAAATTGTTCCCATTAATGAAGGCG
ATGTTCATTGAAACCAACCCAATTCCTGTTAAAACTGCCTTAAATATGATGGGAAGACCTGCTGGCGAGTTAAGATTGCC
ATTATGTGAGATGAGTGAAGAGCATAAAAAGATTTTGGAAAATGTTTTAAAAGATCTTGGTTTAATTTAA

Upstream 100 bases:

>100_bases
GTTTTAGAGGAAGTTGCTCAAATCTCAACAAAAAGATTAAGAAATAGAATTGCAGGATATATAACTCACAAAATGAGACA
GCTCCAATAAAGGTGAAGCT

Downstream 100 bases:

>100_bases
CTTTTATGGTGAAGTTTAATGGATGAAAAAACCATTGAAAAAATAAAAAAAGAGGCTGAAGAGATTATTAATAAATTTAG
CGAGGTATTAGAGAAGTTCA

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFKGVYPAIITPFKNKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIEKVVDVVNGRVQVIAGAGSNC
TEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGKVAESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAV
KEANPNLSQVSELIHDAKITVLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKA
MFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLKDLGLI

Sequences:

>Translated_289_residues
MFKGVYPAIITPFKNKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIEKVVDVVNGRVQVIAGAGSNC
TEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGKVAESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAV
KEANPNLSQVSELIHDAKITVLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKA
MFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLKDLGLI
>Mature_289_residues
MFKGVYPAIITPFKNKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIEKVVDVVNGRVQVIAGAGSNC
TEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGKVAESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAV
KEANPNLSQVSELIHDAKITVLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKA
MFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLKDLGLI

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=283, Percent_Identity=28.2685512367491, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI13540533, Length=300, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1788823, Length=294, Percent_Identity=44.5578231292517, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1786463, Length=284, Percent_Identity=34.1549295774648, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI87082415, Length=296, Percent_Identity=31.7567567567568, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789620, Length=273, Percent_Identity=27.8388278388278, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_METJA (Q57695)

Other databases:

- EMBL:   L77117
- PIR:   E64330
- RefSeq:   NP_247215.1
- PDB:   2YXG
- PDBsum:   2YXG
- ProteinModelPortal:   Q57695
- SMR:   Q57695
- GeneID:   1451098
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0244
- NMPDR:   fig|243232.1.peg.252
- TIGR:   MJ0244
- HOGENOM:   HBG358848
- OMA:   PAIITPF
- ProtClustDB:   PRK03170
- BioCyc:   MJAN243232:MJ_0244-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31580; Mature: 31580

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGVYPAIITPFKNKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIE
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHEEECCCCEEEEEECCCCCCCCCHHHHHHHHH
KVVDVVNGRVQVIAGAGSNCTEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGK
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
VAESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAVKEANPNLSQVSELIHDAKIT
HHHHCCCCEEEEECCCCCEECCCHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHCCEEE
VLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKA
EEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLKDLGLI
HHHCCCCCCHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKGVYPAIITPFKNKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIE
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHEEECCCCEEEEEECCCCCCCCCHHHHHHHHH
KVVDVVNGRVQVIAGAGSNCTEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGK
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
VAESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAVKEANPNLSQVSELIHDAKIT
HHHHCCCCEEEEECCCCCEECCCHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHCCEEE
VLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKA
EEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLKDLGLI
HHHCCCCCCHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087