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The map label for this gene is gatB

Identifier: 15668332

GI number: 15668332

Start: 163090

End: 164508

Strand: Reverse

Name: gatB

Synonym: MJ0160

Alternate gene names: 15668332

Gene position: 164508-163090 (Counterclockwise)

Preceding gene: 15668333

Following gene: 15668331

Centisome position: 9.88

GC content: 35.45

Gene sequence:

>1419_bases
ATGGGTGGAAATATGGAAGATGTTAAAATGAAGTGTGGTTTGGAGATACATGTTCAAATTGATACAAAATCAAAATTATT
CTGTAACTGCTCAACGAATTATTTAGATGCAGAGCCAAACACGAATGTTTGTCCTGTCTGTCTTGGATTGCCTGGAGCAA
AACCACTCCCACCAAATAAAAAGGCAGTGGAAGTTGCAATAATGGTTGCAAAGATGCTTGGTTGTAAAATAGTTGTTGAT
GAAGATATTTACTTCCAAAGAAAGCATTATGATTATCCAGATTTACCGAGCGGTTATCAGAGAACTTCAACCCCTATTGG
AGTAGATGGAGAGTTTATGGGTATTGGAATACATGAGGTTCATTTAGAGGAAGACCCTGGGCAGTACAACCCAAGTTTTG
GAATTGTTGATTATAACAGAAGCGGAACCCCACTAATTGAGATTGTTACAAAGCCAGATATAAAAAGCCCAGAAGAAGCA
AGAGAATTTTTAAAGCAATTGATGACATTATTCAGATACCTTGGCTGTTTAAGAGGAGAAGGAACAATGAGGGCTGATGT
AAATATTTCCATTGAATATATGGGAGTCCAAGGAAATAGGGTTGAGGTTAAAAACGTCAATTCAATTAAAGGGGTTTATA
AAGTTCTAAAATATGAACTAATCAGACAGAAAAACATTATTAAAAGAGGGGGAGAGGTTAAAAGAGAAACAAGAGCATTC
TTAGAAAGTCAGATGATAACTAAGGCAATGAGAAGTAAAGAGACTGCTGAAGATTACAGATATATTCCAGACCCAGACAT
TCAGCCAATAGTCATCTCTGAAAAATGGGTTAAGGAAATAGAGGAAAAAATGCCAGAAACACCATTAGCTAAGAAGAAAA
GATTTGTTGAAGAGTATGGTATTGATGAAGAGGATGCTAAGGTATTAGTTTCTGACTTAGATATGGCTGAAATGTTTGAG
GAAGTTGTTAAATCCTTAGGTGTTAATAAGGAAAATGTTGATTTGGCAGTTACATGGATTAGAAATGAGTTGAGGAGGTC
TTTACAGTATCACAAAGTAGATTTGTATGAGAGTGGGGTTAAGGCAGAGCATATAGTTGAATTAATAAAGCTAATTAAAG
AGGGGGTTATATCTCAAAAAATAGCTAAAGAGATTGTTGATTTGTTGGTTATAAATAGAGGAAAGAAGATGCCTAAAGAA
CTCGTTGAGGAGCTTGGATTAACAGTTATTAGAGATGAAGACGCTTTAGTTAAAGCGGTTGAGGAAGCTATTAAAAACAA
TCCAAAGGCAGTTGAAGATTATCTAAATGGTAAAAAAGAGGCATTGAACTTCTTAATGGGGCAAGTAATGAGATTAACAA
GGGGAAGGGCAGATCCAAAGAGAGTCATTGAGTTATTGAAAGAGAGATTAGATAAATAA

Upstream 100 bases:

>100_bases
TAAAATGAAGTGTGGTTTGGAAATGCACGTGAAAATTTAATAAAGTTTCATCAAAACTAACACCTCCTCGCTTACGCTCG
GAGGTGTAAATTAAAATTTA

Downstream 100 bases:

>100_bases
ATTTTTATTATTCTTTTTTTTAATAATTATTTTTTAGGTGATAATAATGGCAGACCTTGATAGGAAGTTAATAGAAATTT
TAGATATTTTATCTAAATCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MGGNMEDVKMKCGLEIHVQIDTKSKLFCNCSTNYLDAEPNTNVCPVCLGLPGAKPLPPNKKAVEVAIMVAKMLGCKIVVD
EDIYFQRKHYDYPDLPSGYQRTSTPIGVDGEFMGIGIHEVHLEEDPGQYNPSFGIVDYNRSGTPLIEIVTKPDIKSPEEA
REFLKQLMTLFRYLGCLRGEGTMRADVNISIEYMGVQGNRVEVKNVNSIKGVYKVLKYELIRQKNIIKRGGEVKRETRAF
LESQMITKAMRSKETAEDYRYIPDPDIQPIVISEKWVKEIEEKMPETPLAKKKRFVEEYGIDEEDAKVLVSDLDMAEMFE
EVVKSLGVNKENVDLAVTWIRNELRRSLQYHKVDLYESGVKAEHIVELIKLIKEGVISQKIAKEIVDLLVINRGKKMPKE
LVEELGLTVIRDEDALVKAVEEAIKNNPKAVEDYLNGKKEALNFLMGQVMRLTRGRADPKRVIELLKERLDK

Sequences:

>Translated_472_residues
MGGNMEDVKMKCGLEIHVQIDTKSKLFCNCSTNYLDAEPNTNVCPVCLGLPGAKPLPPNKKAVEVAIMVAKMLGCKIVVD
EDIYFQRKHYDYPDLPSGYQRTSTPIGVDGEFMGIGIHEVHLEEDPGQYNPSFGIVDYNRSGTPLIEIVTKPDIKSPEEA
REFLKQLMTLFRYLGCLRGEGTMRADVNISIEYMGVQGNRVEVKNVNSIKGVYKVLKYELIRQKNIIKRGGEVKRETRAF
LESQMITKAMRSKETAEDYRYIPDPDIQPIVISEKWVKEIEEKMPETPLAKKKRFVEEYGIDEEDAKVLVSDLDMAEMFE
EVVKSLGVNKENVDLAVTWIRNELRRSLQYHKVDLYESGVKAEHIVELIKLIKEGVISQKIAKEIVDLLVINRGKKMPKE
LVEELGLTVIRDEDALVKAVEEAIKNNPKAVEDYLNGKKEALNFLMGQVMRLTRGRADPKRVIELLKERLDK
>Mature_471_residues
GGNMEDVKMKCGLEIHVQIDTKSKLFCNCSTNYLDAEPNTNVCPVCLGLPGAKPLPPNKKAVEVAIMVAKMLGCKIVVDE
DIYFQRKHYDYPDLPSGYQRTSTPIGVDGEFMGIGIHEVHLEEDPGQYNPSFGIVDYNRSGTPLIEIVTKPDIKSPEEAR
EFLKQLMTLFRYLGCLRGEGTMRADVNISIEYMGVQGNRVEVKNVNSIKGVYKVLKYELIRQKNIIKRGGEVKRETRAFL
ESQMITKAMRSKETAEDYRYIPDPDIQPIVISEKWVKEIEEKMPETPLAKKKRFVEEYGIDEEDAKVLVSDLDMAEMFEE
VVKSLGVNKENVDLAVTWIRNELRRSLQYHKVDLYESGVKAEHIVELIKLIKEGVISQKIAKEIVDLLVINRGKKMPKEL
VEELGLTVIRDEDALVKAVEEAIKNNPKAVEDYLNGKKEALNFLMGQVMRLTRGRADPKRVIELLKERLDK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=32.5955734406439, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI115534094, Length=390, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6319391, Length=530, Percent_Identity=31.3207547169811, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=32.1792260692464, Blast_Score=231, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_METJA (Q57624)

Other databases:

- EMBL:   L77117
- PIR:   A64320
- RefSeq:   NP_247128.1
- ProteinModelPortal:   Q57624
- SMR:   Q57624
- GeneID:   1451007
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0160
- NMPDR:   fig|243232.1.peg.165
- TIGR:   MJ0160
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   MJAN243232:MJ_0160-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53737; Mature: 53606

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGNMEDVKMKCGLEIHVQIDTKSKLFCNCSTNYLDAEPNTNVCPVCLGLPGAKPLPPNK
CCCCCCHHEEECCEEEEEEEECCCEEEEECCCCEECCCCCCCCCEEEEECCCCCCCCCCC
KAVEVAIMVAKMLGCKIVVDEDIYFQRKHYDYPDLPSGYQRTSTPIGVDGEFMGIGIHEV
HHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
HLEEDPGQYNPSFGIVDYNRSGTPLIEIVTKPDIKSPEEAREFLKQLMTLFRYLGCLRGE
EECCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GTMRADVNISIEYMGVQGNRVEVKNVNSIKGVYKVLKYELIRQKNIIKRGGEVKRETRAF
CCEEEEEEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LESQMITKAMRSKETAEDYRYIPDPDIQPIVISEKWVKEIEEKMPETPLAKKKRFVEEYG
HHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHC
IDEEDAKVLVSDLDMAEMFEEVVKSLGVNKENVDLAVTWIRNELRRSLQYHKVDLYESGV
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
KAEHIVELIKLIKEGVISQKIAKEIVDLLVINRGKKMPKELVEELGLTVIRDEDALVKAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCHHHHHHH
EEAIKNNPKAVEDYLNGKKEALNFLMGQVMRLTRGRADPKRVIELLKERLDK
HHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGNMEDVKMKCGLEIHVQIDTKSKLFCNCSTNYLDAEPNTNVCPVCLGLPGAKPLPPNK
CCCCCHHEEECCEEEEEEEECCCEEEEECCCCEECCCCCCCCCEEEEECCCCCCCCCCC
KAVEVAIMVAKMLGCKIVVDEDIYFQRKHYDYPDLPSGYQRTSTPIGVDGEFMGIGIHEV
HHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
HLEEDPGQYNPSFGIVDYNRSGTPLIEIVTKPDIKSPEEAREFLKQLMTLFRYLGCLRGE
EECCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GTMRADVNISIEYMGVQGNRVEVKNVNSIKGVYKVLKYELIRQKNIIKRGGEVKRETRAF
CCEEEEEEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LESQMITKAMRSKETAEDYRYIPDPDIQPIVISEKWVKEIEEKMPETPLAKKKRFVEEYG
HHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHC
IDEEDAKVLVSDLDMAEMFEEVVKSLGVNKENVDLAVTWIRNELRRSLQYHKVDLYESGV
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
KAEHIVELIKLIKEGVISQKIAKEIVDLLVINRGKKMPKELVEELGLTVIRDEDALVKAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCHHHHHHH
EEAIKNNPKAVEDYLNGKKEALNFLMGQVMRLTRGRADPKRVIELLKERLDK
HHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087