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Identifier: 15645823

GI number: 15645823

Start: 1286191

End: 1286709

Strand: Reverse

Name: Not Available

Synonym: HP1209

Alternate gene names: 15645823

Gene position: 1286709-1286191 (Counterclockwise)

Preceding gene: 15645824

Following gene: 15645822

Centisome position: 77.15

GC content: 36.22

Gene sequence:

>519_bases
TTGGAGTTTGATAAAGGGCAAACTCTAGGGAATTTTATTGATAGAATACGCTTGAATGGCTATAATACCGAATGTGTTTT
TAACCAAAGTATCTGTCAAGACATTAAAAACCACTATAAGCAACAATGTTGTGCGATGTGTGGTGTGCGTGGCAACTCTG
AAAACACTCAAATAGAGGTGGATCATAAAGACGGCCGCAAGGATGATTCAAGAGTTTCTGATTTAAGCACACAAGCTTTT
GATGATTTCCAGGCTTTATGTAAAGCTTGTAATGATAAGAAACGCCAGATTTGTAAAAAATGCAAAGAGACTGGCTATAG
ATTTGATGCAAGAAAAATTCCTGGCAATCATTATTCTTTCTATGAAGGGGAGGCTGAATATGATGGTTGTGTGGGCTGTT
ATCAATATGACCCCATACAATACAGGAAAACTTGTAATGATAGGATATACAATGAAGGGTATCAAAAAGGCTATAGTGAT
GGGTATCAAATTGGATACCATCAAAAAACTACTTTATAG

Upstream 100 bases:

>100_bases
AGTAAGCGTTACAGAATTTGTAGAAAAATACCAAGGATTACAGCTAGGTAATGGGGGGAGTTGGTGTAGGAATAACTCAT
CTTTAGCTAAAGAATTTAAT

Downstream 100 bases:

>100_bases
CGGTTGCAATGAACTACATCGGTTCTAAATACAAGCTCATTCCCTTTATTAAGGAAAATATCCATGCGGTTGTAGGGGAC
AACCTTTCTGGCACGATTTT

Product: ulcer-associated gene restriction endonuclease (iceA)

Products: NA

Alternate protein names: R.NlaIII; Endonuclease NlaIII; Type II restriction enzyme NlaIII [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MEFDKGQTLGNFIDRIRLNGYNTECVFNQSICQDIKNHYKQQCCAMCGVRGNSENTQIEVDHKDGRKDDSRVSDLSTQAF
DDFQALCKACNDKKRQICKKCKETGYRFDARKIPGNHYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIYNEGYQKGYSD
GYQIGYHQKTTL

Sequences:

>Translated_172_residues
MEFDKGQTLGNFIDRIRLNGYNTECVFNQSICQDIKNHYKQQCCAMCGVRGNSENTQIEVDHKDGRKDDSRVSDLSTQAF
DDFQALCKACNDKKRQICKKCKETGYRFDARKIPGNHYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIYNEGYQKGYSD
GYQIGYHQKTTL
>Mature_172_residues
MEFDKGQTLGNFIDRIRLNGYNTECVFNQSICQDIKNHYKQQCCAMCGVRGNSENTQIEVDHKDGRKDDSRVSDLSTQAF
DDFQALCKACNDKKRQICKKCKETGYRFDARKIPGNHYSFYEGEAEYDGCVGCYQYDPIQYRKTCNDRIYNEGYQKGYSD
GYQIGYHQKTTL

Specific function: Recognizes the double-stranded sequence CATG and cleaves after G-4 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR007979 [H]

Pfam domain/function: PF05315 ICEA [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 20006; Mature: 20006

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
7.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDKGQTLGNFIDRIRLNGYNTECVFNQSICQDIKNHYKQQCCAMCGVRGNSENTQIEV
CCCCCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DHKDGRKDDSRVSDLSTQAFDDFQALCKACNDKKRQICKKCKETGYRFDARKIPGNHYSF
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCCCCCEE
YEGEAEYDGCVGCYQYDPIQYRKTCNDRIYNEGYQKGYSDGYQIGYHQKTTL
ECCCCCCCCEEECEECCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MEFDKGQTLGNFIDRIRLNGYNTECVFNQSICQDIKNHYKQQCCAMCGVRGNSENTQIEV
CCCCCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DHKDGRKDDSRVSDLSTQAFDDFQALCKACNDKKRQICKKCKETGYRFDARKIPGNHYSF
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCCCCCEE
YEGEAEYDGCVGCYQYDPIQYRKTCNDRIYNEGYQKGYSDGYQIGYHQKTTL
ECCCCCCCCEEECEECCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8996109 [H]