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The map label for this gene is pyrG

Identifier: 15644977

GI number: 15644977

Start: 356450

End: 358066

Strand: Reverse

Name: pyrG

Synonym: HP0349

Alternate gene names: 15644977

Gene position: 358066-356450 (Counterclockwise)

Preceding gene: 15644985

Following gene: 15644976

Centisome position: 21.47

GC content: 42.05

Gene sequence:

>1617_bases
ATGGATAGAGCCAAATTTATATTCGTTACAGGGGGTGTGTTAAGCTCTCTAGGGAAAGGGATTTCATCTTCTTCAATCGC
TACGCTTTTACAGCATTGCAATTACCAGGTTTCTATTTTGAAGATCGACCCTTATATCAATATTGATCCAGGCACCATGA
GCCCTTTAGAGCATGGGGAAGTGTTTGTAACTAGCGATGGCGCTGAAACGGATTTAGACATAGGGCATTATGAACGCTTT
TTGAACAGGAATTTAACGAGGTTGAATAATTTCACTACTGGGCAGATTTTTTCAAGCGTGATAGAAAATGAAAGGAAAGG
GGAATATTTAGGCAAAACCATTCAAATCGTCCCCCATGTAACCGATGAAATCAAAAGGCGCATTAAAAGCGCGGCTAAGG
GGTTGGATTTTTTAATCGTGGAAGTGGGCGGAACCGTGGGCGATATGGAGGGCATGTTTTATTTGGAAGCGATCCGCCAG
CTTAAATTGGAATTAGGGAATGAAAAAGTCATCAATGTGCATGTAACCCTGATCCCCTATATCCAAACCACTAACGAACT
AAAAACCAAACCCACGCAACATTCCGTCCAAGAATTAAGACGCCTTGGCGTAACCCCTCAAATCATTTTGGCGCGATCGC
CTAAGCCTTTGGATAAAGAGTTGAAAAATAAAATCGCTTTGAGTTGCGATGTGGAGCAAGACAGCGTGATTGTCGCCACA
GACACTAAAAGCATTTACGCATGCCCTATTCTTTTCTTGCAAGAAGGCATTTTAACCCCCATTGCCAGACGCTTTAATTT
GAATAAACTACACCCCAAAATGGCGGCTTGGAACACTTTAGTAGAAAAGATTATCGCTCCTAAACACAAAGTCAAAATTG
GTTTTGTGGGCAAGTATTTAAGCTTGAAAGAATCTTATAAATCCTTGATTGAAGCCCTAATCCATGCGGGCGCGCATCTG
GATACGCAAGTCAATATTGAATGGCTGGATAGCGAGAATTTTAATGAAAAGACGGATTTAGAGGGCGTTGATGCGATTTT
AGTGCCTGGGGGCTTTGGAGAAAGGGGGATTGAGGGCAAAATTTGCGCCATTCAAAGGGCTAGGTTAGAAAAACTCCCCT
TTTTAGGGATTTGTTTGGGCATGCAATTAGCGATCGTTGAATTTTGTCGCAATGTTTTGGGTTTAAAAGGGGCTAACTCT
ACGGAGTTTAACCAACGCTGCGAATACCCTGTGGTGTATTTGATTGGAGATTTTATGGATCAAAACCACCAAAAACAGGT
GCGCACCTATAATTCGCCTTTAGGAGGCACCATGCGATTAGGCGAATACGAATGCGAAATTATGCCAAACAGCTTGTTAG
AAAAAGCTTATAAAAAGCCTAGCATTAAAGAAAGACACCGCCATCGTTATGAAATCAACCCCAAATACCGCCAAGAGTGG
GAAAATAAGGGCTTGAAAGTGGTGGGTTTTGGATCGAATCATTTGATTGAAGCGATTGAATTAGAAGATCACCCGTTCTT
TGTGGGGGTGCAATTCCACCCAGAATTCACCTCCAGGTTGCAAAGCCCTAACCCTATTATTTTAGATTTCATTAAGAGCG
CTCTTTCTAAATCCTAA

Upstream 100 bases:

>100_bases
TTTTTTTTGAAATCGCTTTAATTTTTGCCCTCTTTTAAAGCTTTTTAACCCTTTTTAGACTACAATTTAAGAAGCAAAAA
TTAAAGGAACGCAATAACTT

Downstream 100 bases:

>100_bases
TCTAACAAATGAAACAAAAGCTTAAAGCTCAAATCAAAGAGCGCATGGCTTCTATCGCTTATAATGAAAAAGGGTTTCCT
AGCCCCTTTTTATTTAAAGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DTQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIGDFMDQNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ENKGLKVVGFGSNHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS

Sequences:

>Translated_538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DTQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIGDFMDQNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ENKGLKVVGFGSNHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS
>Mature_538_residues
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGEVFVTSDGAETDLDIGHYERF
LNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHVTDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQ
LKLELGNEKVINVHVTLIPYIQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYLSLKESYKSLIEALIHAGAHL
DTQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANS
TEFNQRCEYPVVYLIGDFMDQNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
ENKGLKVVGFGSNHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=44.6428571428571, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=43.4470377019749, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=43.4470377019749, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=43.4470377019749, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI1789142, Length=532, Percent_Identity=49.0601503759398, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.5878489326765, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=571, Percent_Identity=40.9807355516637, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=571, Percent_Identity=40.4553415061296, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=44.1071428571429, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.1782178217822, Blast_Score=373, Evalue=1e-103,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60617; Mature: 60617

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGE
CCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCC
VFVTSDGAETDLDIGHYERFLNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHV
EEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCHHCCEEEEECCC
TDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQLKLELGNEKVINVHVTLIPY
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
EECCCHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEEE
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLKESYKSLIEALIHAGAHLDTQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGK
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANSTEFNQRCEYPVVYLIGDFMD
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEHHHC
QNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
CCCHHHHHHCCCCCCCCEEECCEEEEECCHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHH
ENKGLKVVGFGSNHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS
HHCCCEEEEECCCCEEEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDRAKFIFVTGGVLSSLGKGISSSSIATLLQHCNYQVSILKIDPYINIDPGTMSPLEHGE
CCCCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCC
VFVTSDGAETDLDIGHYERFLNRNLTRLNNFTTGQIFSSVIENERKGEYLGKTIQIVPHV
EEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCHHCCEEEEECCC
TDEIKRRIKSAAKGLDFLIVEVGGTVGDMEGMFYLEAIRQLKLELGNEKVINVHVTLIPY
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
IQTTNELKTKPTQHSVQELRRLGVTPQIILARSPKPLDKELKNKIALSCDVEQDSVIVAT
EECCCHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCEEEEEECCCCCEEEEE
DTKSIYACPILFLQEGILTPIARRFNLNKLHPKMAAWNTLVEKIIAPKHKVKIGFVGKYL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
SLKESYKSLIEALIHAGAHLDTQVNIEWLDSENFNEKTDLEGVDAILVPGGFGERGIEGK
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
ICAIQRARLEKLPFLGICLGMQLAIVEFCRNVLGLKGANSTEFNQRCEYPVVYLIGDFMD
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEHHHC
QNHQKQVRTYNSPLGGTMRLGEYECEIMPNSLLEKAYKKPSIKERHRHRYEINPKYRQEW
CCCHHHHHHCCCCCCCCEEECCEEEEECCHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHH
ENKGLKVVGFGSNHLIEAIELEDHPFFVGVQFHPEFTSRLQSPNPIILDFIKSALSKS
HHCCCEEEEECCCCEEEEEEECCCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA