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Identifier: 15644678

GI number: 15644678

Start: 45041

End: 46039

Strand: Reverse

Name: Not Available

Synonym: HP0047

Alternate gene names: 15644678

Gene position: 46039-45041 (Counterclockwise)

Preceding gene: 15644679

Following gene: 15644663

Centisome position: 2.76

GC content: 41.34

Gene sequence:

>999_bases
ATGGATAGCGTAACTCTAGCATGCGGGAACGGAGGGAAAGAAACAAACGCTTTGATTGAGCGAGTCTTTATGCCCTATTT
AAAAGAATGGATTGTTGCATTTGATGAAGACGCCCCTAAATTTGAAGCTAGTGGGGAATATTGCGTGAGCACGGATAGTT
TTGTCATCACGCCCTTAATTTTTAATGGGGGCGATATAGGCAAGCTTTGCGTTTGCGGGAGTGCGAATGATGTGAGCGTG
CAAGGGGGCGAACCTTTGTATTTGAATATGGGTTTTATTTTAGAAGAAGGCTTAGAAATTTCTCTTTTAAAACAAATTTT
ACAATCCATACAAAAAGAATTGTTTAAAGCCAACCTGAAACTCCTCTCCCTAGACACTAAAGTCGTGCCAAAGGGGAGCG
TGGATAAGCTTTTTATCAACACAACCTGCATTGGTAAAATCATCAAGCCAGGGATTTCTTCGTACCATTTACAACAAGGG
CAAGCCATTATCCTAAGCGACACTATCGCCAATCATGGGGCAAGCTTATTTGCGATGCGTAATGAAATCAAGCTTAAAAC
GAATCTAGAAAGCGATTGCCAACTGCTCTATCCCTTATTAAAACCCCTATTTTTAAGCGATCTCAAAATTGATGCTTTAA
GAGATGCGACTAGGGGCGGGTTAGCGAGCGTGCTGAACGAATGGGCGAACAGCTCTAGAGTGAAAATCGTTATAGAAGAA
GAAAAAATCCCCTTAAAAGAAGAAACGAAAGGGATTTGTGAGATTTTAGGGTTAGAACCCTACGCGCTAGCCAATGAGGG
GGTGTTTGTTTTAGCGCTCAATCAAAAAGACGCCCCTAAAGCCTTAGAAATTTTAAAAAGTAACGAAAAAGCTAAAAACG
CTTGCGTGATTGGCAAAGTGTTTGAAAACCCTTATCCTAGCGTGGTTTTAAAGAACGCATGGGGTTTTGAAAGGATTTTA
GAGGTGCCAGAGGGCGAATTATTGCCTAGGATTTGTTAA

Upstream 100 bases:

>100_bases
ATTTTTTCCACAAGCATTTCCCCCCTAATGACAGCAGTATCCCTATCGGTCAAGCCTTAATGGCGTATTTCAACCCTACA
ATCATCAAAAAAGGATAAAA

Downstream 100 bases:

>100_bases
CACGCCGTCATTTTTTAATCGTTTTAAGCCTGCCCTAAAAATGGTTTAGAGTCCGCTTAAGCTAAGTCTAAGTCTACTAT
ACTCAAGGCATTTGAGAAAA

Product: hydrogenase expression/formation protein (hypE)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDSVTLACGNGGKETNALIERVFMPYLKEWIVAFDEDAPKFEASGEYCVSTDSFVITPLIFNGGDIGKLCVCGSANDVSV
QGGEPLYLNMGFILEEGLEISLLKQILQSIQKELFKANLKLLSLDTKVVPKGSVDKLFINTTCIGKIIKPGISSYHLQQG
QAIILSDTIANHGASLFAMRNEIKLKTNLESDCQLLYPLLKPLFLSDLKIDALRDATRGGLASVLNEWANSSRVKIVIEE
EKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNACVIGKVFENPYPSVVLKNAWGFERIL
EVPEGELLPRIC

Sequences:

>Translated_332_residues
MDSVTLACGNGGKETNALIERVFMPYLKEWIVAFDEDAPKFEASGEYCVSTDSFVITPLIFNGGDIGKLCVCGSANDVSV
QGGEPLYLNMGFILEEGLEISLLKQILQSIQKELFKANLKLLSLDTKVVPKGSVDKLFINTTCIGKIIKPGISSYHLQQG
QAIILSDTIANHGASLFAMRNEIKLKTNLESDCQLLYPLLKPLFLSDLKIDALRDATRGGLASVLNEWANSSRVKIVIEE
EKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNACVIGKVFENPYPSVVLKNAWGFERIL
EVPEGELLPRIC
>Mature_332_residues
MDSVTLACGNGGKETNALIERVFMPYLKEWIVAFDEDAPKFEASGEYCVSTDSFVITPLIFNGGDIGKLCVCGSANDVSV
QGGEPLYLNMGFILEEGLEISLLKQILQSIQKELFKANLKLLSLDTKVVPKGSVDKLFINTTCIGKIIKPGISSYHLQQG
QAIILSDTIANHGASLFAMRNEIKLKTNLESDCQLLYPLLKPLFLSDLKIDALRDATRGGLASVLNEWANSSRVKIVIEE
EKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNACVIGKVFENPYPSVVLKNAWGFERIL
EVPEGELLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=342, Percent_Identity=40.6432748538012, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 36406; Mature: 36406

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSVTLACGNGGKETNALIERVFMPYLKEWIVAFDEDAPKFEASGEYCVSTDSFVITPLI
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEEE
FNGGDIGKLCVCGSANDVSVQGGEPLYLNMGFILEEGLEISLLKQILQSIQKELFKANLK
ECCCCCCEEEEECCCCCEEECCCCEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHCCCE
LLSLDTKVVPKGSVDKLFINTTCIGKIIKPGISSYHLQQGQAIILSDTIANHGASLFAMR
EEEECCEECCCCCCCEEEEEHHHHHHHHCCCCCHHHHCCCCEEEEECCHHHCCCEEEEEE
NEIKLKTNLESDCQLLYPLLKPLFLSDLKIDALRDATRGGLASVLNEWANSSRVKIVIEE
CCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEC
EKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNACVIGKV
CCCCCHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEHH
FENPYPSVVLKNAWGFERILEVPEGELLPRIC
HCCCCCCEEEECCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDSVTLACGNGGKETNALIERVFMPYLKEWIVAFDEDAPKFEASGEYCVSTDSFVITPLI
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEEE
FNGGDIGKLCVCGSANDVSVQGGEPLYLNMGFILEEGLEISLLKQILQSIQKELFKANLK
ECCCCCCEEEEECCCCCEEECCCCEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHCCCE
LLSLDTKVVPKGSVDKLFINTTCIGKIIKPGISSYHLQQGQAIILSDTIANHGASLFAMR
EEEECCEECCCCCCCEEEEEHHHHHHHHCCCCCHHHHCCCCEEEEECCHHHCCCEEEEEE
NEIKLKTNLESDCQLLYPLLKPLFLSDLKIDALRDATRGGLASVLNEWANSSRVKIVIEE
CCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEC
EKIPLKEETKGICEILGLEPYALANEGVFVLALNQKDAPKALEILKSNEKAKNACVIGKV
CCCCCHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEHH
FENPYPSVVLKNAWGFERILEVPEGELLPRIC
HCCCCCCEEEECCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]