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The map label for this gene is pcnB_1

Identifier: 15618754

GI number: 15618754

Start: 956847

End: 958079

Strand: Reverse

Name: pcnB_1

Synonym: CPn0845

Alternate gene names: 15618754

Gene position: 958079-956847 (Counterclockwise)

Preceding gene: 15618755

Following gene: 15618753

Centisome position: 77.88

GC content: 42.01

Gene sequence:

>1233_bases
ATGACAACAATTGCCATAGAAGCTGCAAAAAAAGTTCTTATCAAACTACGTAATGCAGGATATCAGGCATATTTTGTCGG
TGGTTGTGTTCGAGATATGTTAATGAATCGCCCTCTCGAGGATATTGACATAGCTACCAATGCATCCCCAACGATTGTAT
CTACGATCTTCCCAGACGTCATCAGTATTGGTGTTGCCTTTGGTATCATCGTAGTAAAACAGGACGGACGCCTATTTGAA
GTTGCAACTTTCCGCTCTGACGGTGAATACAAAGATGGAAGGCATCCCGATCGTATCATCTTTTCTTCTATGCGAGAAGA
TGCCCTCCGAAGAGACTTCACAGTAAACGGGATGTACTACGATCCTTTTGAAGACAAAGTTTTCGACTTTGTCGAAGGAA
CTCGTGATATTGAAAAGAAAGTCATTCGAGCTATAGGCCATCCACGTTTAAGATTCTCAGAAGATAAACTGCGTATCCTA
CGTGCCATACGTTTTTCTTCCTCCCTCGGCTTCACCCTAGACCCAACTACAGAACGCGCCATTATTAAAGAAGCCCCGGC
ATTAGTAAATTCCGTATCCCCGGAGAGAATCTGGCAAGAGCTAAAAAAAATGCTTAAAAGACAGCCTTATGGAGCCCTCT
CTCTTCTGCTCAAGCTTAAAGTCTTAATTTTTATTTTCCCTGAACTACGTGATATTCCCTACAGTCTCTTAAGAACAACC
ATAGAATTTGCCCGTAAGTTTAATCCAACACATTTCCCTGAAATTCTTTTTTTACTGCCTCTATTCCAAGGGGTCAGTGA
GGAAGCCGCAACAGTAGCGTTTGGAAGATTACGAATTTCTAATAAAGAATTGAAATTAATAGAATCTTGGTACGAGGCAC
TCCCTCATTTCCAAAACCAATCCGGCAATCGTGTATTTTGGGCGCATTTCCTAGCGTCTCCGACAGCACCTCTATTTTTG
GAGCTCTTCTCGGCTCTACAAAAAGATCCGAGCAGACAGCAGCACTTTATCTCTCGAGTGCAAGAACTCGAATCACGCTT
AGAGCAATTCATATTAAGAATAAAAACATCCTCCCCCGTTGTTTCGGCTCCTGATCTGATAGCTAAGGGCATCTCTCCCG
GAAGACTTCTTGGCGATCTACTCAGAGAAGCTGAGATTCTCTCTATAGAAAATGAATGTCTCGACAAAGAAAAAATTTTA
TTGCTTTTACAAGAAAAAGGCTTTTGGAAGTAG

Upstream 100 bases:

>100_bases
TCCAAGAAGACACTATCGCAGAAAATAAAGCGCCAATAATTATCAGAAGGACCCCAGAAGCTATCGCTTAGCTCTTTTTA
GTTCCTATTTTAGGGGTGTC

Downstream 100 bases:

>100_bases
ATTAATTTAAATAGAATCCTACTTTTACAACCCTTAGAAATTTATCTCTTTAGCTTTAATTTTTTAACTCCTATTATTTT
CTCATTAATTACTGAAAAAA

Product: Poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTTIAIEAAKKVLIKLRNAGYQAYFVGGCVRDMLMNRPLEDIDIATNASPTIVSTIFPDVISIGVAFGIIVVKQDGRLFE
VATFRSDGEYKDGRHPDRIIFSSMREDALRRDFTVNGMYYDPFEDKVFDFVEGTRDIEKKVIRAIGHPRLRFSEDKLRIL
RAIRFSSSLGFTLDPTTERAIIKEAPALVNSVSPERIWQELKKMLKRQPYGALSLLLKLKVLIFIFPELRDIPYSLLRTT
IEFARKFNPTHFPEILFLLPLFQGVSEEAATVAFGRLRISNKELKLIESWYEALPHFQNQSGNRVFWAHFLASPTAPLFL
ELFSALQKDPSRQQHFISRVQELESRLEQFILRIKTSSPVVSAPDLIAKGISPGRLLGDLLREAEILSIENECLDKEKIL
LLLQEKGFWK

Sequences:

>Translated_410_residues
MTTIAIEAAKKVLIKLRNAGYQAYFVGGCVRDMLMNRPLEDIDIATNASPTIVSTIFPDVISIGVAFGIIVVKQDGRLFE
VATFRSDGEYKDGRHPDRIIFSSMREDALRRDFTVNGMYYDPFEDKVFDFVEGTRDIEKKVIRAIGHPRLRFSEDKLRIL
RAIRFSSSLGFTLDPTTERAIIKEAPALVNSVSPERIWQELKKMLKRQPYGALSLLLKLKVLIFIFPELRDIPYSLLRTT
IEFARKFNPTHFPEILFLLPLFQGVSEEAATVAFGRLRISNKELKLIESWYEALPHFQNQSGNRVFWAHFLASPTAPLFL
ELFSALQKDPSRQQHFISRVQELESRLEQFILRIKTSSPVVSAPDLIAKGISPGRLLGDLLREAEILSIENECLDKEKIL
LLLQEKGFWK
>Mature_409_residues
TTIAIEAAKKVLIKLRNAGYQAYFVGGCVRDMLMNRPLEDIDIATNASPTIVSTIFPDVISIGVAFGIIVVKQDGRLFEV
ATFRSDGEYKDGRHPDRIIFSSMREDALRRDFTVNGMYYDPFEDKVFDFVEGTRDIEKKVIRAIGHPRLRFSEDKLRILR
AIRFSSSLGFTLDPTTERAIIKEAPALVNSVSPERIWQELKKMLKRQPYGALSLLLKLKVLIFIFPELRDIPYSLLRTTI
EFARKFNPTHFPEILFLLPLFQGVSEEAATVAFGRLRISNKELKLIESWYEALPHFQNQSGNRVFWAHFLASPTAPLFLE
LFSALQKDPSRQQHFISRVQELESRLEQFILRIKTSSPVVSAPDLIAKGISPGRLLGDLLREAEILSIENECLDKEKILL
LLQEKGFWK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=194, Percent_Identity=36.5979381443299, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI87081691, Length=238, Percent_Identity=36.9747899159664, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1789436, Length=213, Percent_Identity=28.6384976525822, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=208, Percent_Identity=37.0192307692308, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321016, Length=227, Percent_Identity=30.3964757709251, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281360112, Length=281, Percent_Identity=33.0960854092527, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357337, Length=281, Percent_Identity=33.0960854092527, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46919; Mature: 46788

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAIEAAKKVLIKLRNAGYQAYFVGGCVRDMLMNRPLEDIDIATNASPTIVSTIFPDV
CCEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
ISIGVAFGIIVVKQDGRLFEVATFRSDGEYKDGRHPDRIIFSSMREDALRRDFTVNGMYY
HHHHHHHEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCEEE
DPFEDKVFDFVEGTRDIEKKVIRAIGHPRLRFSEDKLRILRAIRFSSSLGFTLDPTTERA
CCHHHHHHHHHHCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCEECCCHHHH
IIKEAPALVNSVSPERIWQELKKMLKRQPYGALSLLLKLKVLIFIFPELRDIPYSLLRTT
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IEFARKFNPTHFPEILFLLPLFQGVSEEAATVAFGRLRISNKELKLIESWYEALPHFQNQ
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCCCC
SGNRVFWAHFLASPTAPLFLELFSALQKDPSRQQHFISRVQELESRLEQFILRIKTSSPV
CCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSAPDLIAKGISPGRLLGDLLREAEILSIENECLDKEKILLLLQEKGFWK
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCC
>Mature Secondary Structure 
TTIAIEAAKKVLIKLRNAGYQAYFVGGCVRDMLMNRPLEDIDIATNASPTIVSTIFPDV
CEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
ISIGVAFGIIVVKQDGRLFEVATFRSDGEYKDGRHPDRIIFSSMREDALRRDFTVNGMYY
HHHHHHHEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCEEE
DPFEDKVFDFVEGTRDIEKKVIRAIGHPRLRFSEDKLRILRAIRFSSSLGFTLDPTTERA
CCHHHHHHHHHHCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCEECCCHHHH
IIKEAPALVNSVSPERIWQELKKMLKRQPYGALSLLLKLKVLIFIFPELRDIPYSLLRTT
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IEFARKFNPTHFPEILFLLPLFQGVSEEAATVAFGRLRISNKELKLIESWYEALPHFQNQ
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCCCC
SGNRVFWAHFLASPTAPLFLELFSALQKDPSRQQHFISRVQELESRLEQFILRIKTSSPV
CCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSAPDLIAKGISPGRLLGDLLREAEILSIENECLDKEKILLLLQEKGFWK
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA