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The map label for this gene is carB2

Identifier: 15607056

GI number: 15607056

Start: 1476861

End: 1478534

Strand: Direct

Name: carB2

Synonym: aq_2101

Alternate gene names: 15607056

Gene position: 1476861-1478534 (Clockwise)

Preceding gene: 15607050

Following gene: 15607057

Centisome position: 95.2

GC content: 47.25

Gene sequence:

>1674_bases
ATGCCTAAAAGGACGGACATCAAGAAGATATTAATAATAGGCTCTGGGCCTATCGTTATTGGACAGGCTGCAGAATTCGA
CTACTCGGGAACCCAAGCCTGCAAAGCCCTAAAGCAGGAAGGATACGAAGTCATTCTCGTGAACTCAAATCCCGCAACGA
TTATGACCGACCCAGAAATGGCCGACAAGACCTATATAGAACCCCTTACCGCTGACATACTTGAAGAGATTATAAAGAAA
GAAAGGCCTGACGCCCTCCTTCCCACGCTCGGAGGTCAAACGGGCCTAAACTTGGCGGTGGAACTGTACGAGAACGGTGT
TCTTGAACGCTACGGAGTTGAGCTAATAGGTGCGAACTACGAGGCCATAAAAAAGGGAGAAGACAGAGAACTCTTTAAAG
AAACTATGGAAAGTATAGGGCTGAAGGTTCCGGAAAGTGCTGTCGTTCGCTCTGTAGAAGAAGGACTGAAGGCTGTAGAG
AAGATAGGTTTTCCCGTGATACTGAGACCTGCTTTTACTCTGGGAGGGACCGGAAGCTCGGTTGCCTACAACGTTGAGGA
GTTTAAAGAGAAGTTAAAGGTTGCCCTTGAGACTTCCCCCATTCACGAGGTTTTGCTGGACAAGTCCCTGATCGGCTGGA
AGGAGATAGAGTTTGAAGTTGTAAGAGACAAGGCGGACAATGTCATTATCGTTTGTGCTATAGAGAACTTTGATCCCATG
GGTGTTCACACGGGTGACTCCATAACCGTAGCCCCAACACAGACCTTGACGGACAAAGAGTACCAGATGCTCAGGGACGC
GGCCATTGCGGTAATAAGGGCGATAGGTGTGGATACGGGAGGTTCTAACATACAGTTTGCCCTCTCACCCGAGAGCGGAG
ACTTTTACGTTATTGAGATGAACCCGAGAGTTTCACGCTCTTCAGCTCTAGCGTCAAAAGCAACGGGTTTCCCAATAGCA
AAAGTCGCGGCAAAGCTTGCGGTGGGCTACACCTTGGACGAACTTAAAAACGACATTACGAAGTTCACCCCAGCCTCCTT
TGAACCTTCTATAGACTACGTAGTTGTAAAGATTCCGAGGTTTGACTTTGCAAAGTTCCCCGAAGCGGACAGAACCCTCA
CCACTATGATGAAGTCCGTAGGGGAAGTAATGGCTATAGGAAGAACTTTCAAGGAAGCACTCCTAAAGGCTGTAAGGAGT
TTAGAACTCGACAGGTACGGCCTAGCTTTCCCGAAACTCACGAACGTTGATAAGGTAGAACTCGAAAGGAATTTAATTAA
CCCAAACGACCAGAGACTCTGGTACATAGCTGAAGCCTTCAGGAGAGGGTATTCCGTAGACGAAGTTTACGAACTCACCA
AGATAGACAGATGGTTCCTTTATAACATAGAAGAGATAGTAAAGTTTGAGGAAGTTCTCCGGAAGGAAGAACTTACCCCT
GAAATCCTCAGACAAGCAAAGGAAATGGGCTACTCCGACTGGGAGATAGCAAAAATAAAGGGAATTACGGAAGAAGAAGT
CAGGAAGCTCAGAAAGGAAGAGGGTATAGTCCCCACATTCAAGGGTGTTGACACCTGCGCGGGAGAATTCGTGGCGTATA
CACCCTACTACTATTCCTCCTATGAGAGACCTTACTACACTGTAGACGGGCAGGAAATCCTTGATGAAGATTAA

Upstream 100 bases:

>100_bases
ACGAACACCGCAACGCTTTTCCCTTTGTACTTCTTTATGTCATCTTTGAAAGCCCTTACTTCCATTTCCTTCACCTCACT
GTTTATACTATATACTTTAA

Downstream 100 bases:

>100_bases
AAGGTTTGTTTACTCACTGCCCGAGGAGGAGTTCTACGAACTCGTTTACTCCCAAAACCTTTCCGTTGAGGTCCTTGAAA
GGAAAGAGATCGAAGTAATT

Product: carbamoyl-phosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MPKRTDIKKILIIGSGPIVIGQAAEFDYSGTQACKALKQEGYEVILVNSNPATIMTDPEMADKTYIEPLTADILEEIIKK
ERPDALLPTLGGQTGLNLAVELYENGVLERYGVELIGANYEAIKKGEDRELFKETMESIGLKVPESAVVRSVEEGLKAVE
KIGFPVILRPAFTLGGTGSSVAYNVEEFKEKLKVALETSPIHEVLLDKSLIGWKEIEFEVVRDKADNVIIVCAIENFDPM
GVHTGDSITVAPTQTLTDKEYQMLRDAAIAVIRAIGVDTGGSNIQFALSPESGDFYVIEMNPRVSRSSALASKATGFPIA
KVAAKLAVGYTLDELKNDITKFTPASFEPSIDYVVVKIPRFDFAKFPEADRTLTTMMKSVGEVMAIGRTFKEALLKAVRS
LELDRYGLAFPKLTNVDKVELERNLINPNDQRLWYIAEAFRRGYSVDEVYELTKIDRWFLYNIEEIVKFEEVLRKEELTP
EILRQAKEMGYSDWEIAKIKGITEEEVRKLRKEEGIVPTFKGVDTCAGEFVAYTPYYYSSYERPYYTVDGQEILDED

Sequences:

>Translated_557_residues
MPKRTDIKKILIIGSGPIVIGQAAEFDYSGTQACKALKQEGYEVILVNSNPATIMTDPEMADKTYIEPLTADILEEIIKK
ERPDALLPTLGGQTGLNLAVELYENGVLERYGVELIGANYEAIKKGEDRELFKETMESIGLKVPESAVVRSVEEGLKAVE
KIGFPVILRPAFTLGGTGSSVAYNVEEFKEKLKVALETSPIHEVLLDKSLIGWKEIEFEVVRDKADNVIIVCAIENFDPM
GVHTGDSITVAPTQTLTDKEYQMLRDAAIAVIRAIGVDTGGSNIQFALSPESGDFYVIEMNPRVSRSSALASKATGFPIA
KVAAKLAVGYTLDELKNDITKFTPASFEPSIDYVVVKIPRFDFAKFPEADRTLTTMMKSVGEVMAIGRTFKEALLKAVRS
LELDRYGLAFPKLTNVDKVELERNLINPNDQRLWYIAEAFRRGYSVDEVYELTKIDRWFLYNIEEIVKFEEVLRKEELTP
EILRQAKEMGYSDWEIAKIKGITEEEVRKLRKEEGIVPTFKGVDTCAGEFVAYTPYYYSSYERPYYTVDGQEILDED
>Mature_556_residues
PKRTDIKKILIIGSGPIVIGQAAEFDYSGTQACKALKQEGYEVILVNSNPATIMTDPEMADKTYIEPLTADILEEIIKKE
RPDALLPTLGGQTGLNLAVELYENGVLERYGVELIGANYEAIKKGEDRELFKETMESIGLKVPESAVVRSVEEGLKAVEK
IGFPVILRPAFTLGGTGSSVAYNVEEFKEKLKVALETSPIHEVLLDKSLIGWKEIEFEVVRDKADNVIIVCAIENFDPMG
VHTGDSITVAPTQTLTDKEYQMLRDAAIAVIRAIGVDTGGSNIQFALSPESGDFYVIEMNPRVSRSSALASKATGFPIAK
VAAKLAVGYTLDELKNDITKFTPASFEPSIDYVVVKIPRFDFAKFPEADRTLTTMMKSVGEVMAIGRTFKEALLKAVRSL
ELDRYGLAFPKLTNVDKVELERNLINPNDQRLWYIAEAFRRGYSVDEVYELTKIDRWFLYNIEEIVKFEEVLRKEELTPE
ILRQAKEMGYSDWEIAKIKGITEEEVRKLRKEEGIVPTFKGVDTCAGEFVAYTPYYYSSYERPYYTVDGQEILDED

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=537, Percent_Identity=50.0931098696462, Blast_Score=519, Evalue=1e-147,
Organism=Homo sapiens, GI21361331, Length=559, Percent_Identity=45.6171735241503, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI169790915, Length=559, Percent_Identity=45.6171735241503, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI170295797, Length=526, Percent_Identity=44.4866920152091, Blast_Score=451, Evalue=1e-127,
Organism=Escherichia coli, GI1786216, Length=549, Percent_Identity=62.4772313296903, Blast_Score=692, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=542, Percent_Identity=47.7859778597786, Blast_Score=502, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI133931226, Length=195, Percent_Identity=24.1025641025641, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322331, Length=544, Percent_Identity=50.7352941176471, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6322569, Length=546, Percent_Identity=48.9010989010989, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24642586, Length=544, Percent_Identity=49.0808823529412, Blast_Score=517, Evalue=1e-146,
Organism=Drosophila melanogaster, GI45555749, Length=544, Percent_Identity=49.0808823529412, Blast_Score=516, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24651757, Length=371, Percent_Identity=23.4501347708895, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI281363050, Length=360, Percent_Identity=23.8888888888889, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24652224, Length=360, Percent_Identity=23.8888888888889, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24652222, Length=360, Percent_Identity=23.8888888888889, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24652220, Length=360, Percent_Identity=23.8888888888889, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24652218, Length=360, Percent_Identity=23.8888888888889, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARB1_AQUAE (O67869)

Other databases:

- EMBL:   AE000657
- PIR:   A70480
- RefSeq:   NP_214438.1
- ProteinModelPortal:   O67869
- SMR:   O67869
- GeneID:   1193858
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2101
- NMPDR:   fig|224324.1.peg.1453
- HOGENOM:   HBG405439
- OMA:   EPSAGEF
- ProtClustDB:   CLSK790184
- BioCyc:   AAEO224324:AQ_2101-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01210_B
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:1.10.1030.10
- Gene3D:   G3DSA:3.40.50.20
- PRINTS:   PR00098

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; SSF48108 CarbamoylP_synth_lsu_oligo; SSF52440 PreATP-grasp-like

EC number: =6.3.5.5

Molecular weight: Translated: 62405; Mature: 62274

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRTDIKKILIIGSGPIVIGQAAEFDYSGTQACKALKQEGYEVILVNSNPATIMTDPEM
CCCCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCC
ADKTYIEPLTADILEEIIKKERPDALLPTLGGQTGLNLAVELYENGVLERYGVELIGANY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHCCCHHHHCEEEECCCH
EAIKKGEDRELFKETMESIGLKVPESAVVRSVEEGLKAVEKIGFPVILRPAFTLGGTGSS
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCC
VAYNVEEFKEKLKVALETSPIHEVLLDKSLIGWKEIEFEVVRDKADNVIIVCAIENFDPM
EEECHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHEEEECCCCCEEEEEEECCCCCC
GVHTGDSITVAPTQTLTDKEYQMLRDAAIAVIRAIGVDTGGSNIQFALSPESGDFYVIEM
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEC
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNDITKFTPASFEPSIDYVVVKIPR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECC
FDFAKFPEADRTLTTMMKSVGEVMAIGRTFKEALLKAVRSLELDRYGLAFPKLTNVDKVE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
LERNLINPNDQRLWYIAEAFRRGYSVDEVYELTKIDRWFLYNIEEIVKFEEVLRKEELTP
HHHHCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCH
EILRQAKEMGYSDWEIAKIKGITEEEVRKLRKEEGIVPTFKGVDTCAGEFVAYTPYYYSS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHCCCEEEECCHHHCC
YERPYYTVDGQEILDED
CCCCEEEECCHHHCCCC
>Mature Secondary Structure 
PKRTDIKKILIIGSGPIVIGQAAEFDYSGTQACKALKQEGYEVILVNSNPATIMTDPEM
CCCCCCEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCC
ADKTYIEPLTADILEEIIKKERPDALLPTLGGQTGLNLAVELYENGVLERYGVELIGANY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHCCCHHHHCEEEECCCH
EAIKKGEDRELFKETMESIGLKVPESAVVRSVEEGLKAVEKIGFPVILRPAFTLGGTGSS
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCC
VAYNVEEFKEKLKVALETSPIHEVLLDKSLIGWKEIEFEVVRDKADNVIIVCAIENFDPM
EEECHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHEEEECCCCCEEEEEEECCCCCC
GVHTGDSITVAPTQTLTDKEYQMLRDAAIAVIRAIGVDTGGSNIQFALSPESGDFYVIEM
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEC
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNDITKFTPASFEPSIDYVVVKIPR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECC
FDFAKFPEADRTLTTMMKSVGEVMAIGRTFKEALLKAVRSLELDRYGLAFPKLTNVDKVE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
LERNLINPNDQRLWYIAEAFRRGYSVDEVYELTKIDRWFLYNIEEIVKFEEVLRKEELTP
HHHHCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCH
EILRQAKEMGYSDWEIAKIKGITEEEVRKLRKEEGIVPTFKGVDTCAGEFVAYTPYYYSS
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHCCCEEEECCHHHCC
YERPYYTVDGQEILDED
CCCCEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320