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The map label for this gene is b2999 [C]

Identifier: 15606989

GI number: 15606989

Start: 1404707

End: 1405402

Strand: Direct

Name: b2999 [C]

Synonym: aq_1997

Alternate gene names: 15606989

Gene position: 1404707-1405402 (Clockwise)

Preceding gene: 15606979

Following gene: 15606990

Centisome position: 90.55

GC content: 44.54

Gene sequence:

>696_bases
ATGGGACAAATGGTGGAGTTTGAGAAAAACGGAGTTAAGGTCAGGGGATACATAGCTACTCCGAAGTGGGCTGGTCCTGC
GGTGTTGGTCTTTCACGAGTGGTGGGGGCTTGAAAGTCCGCTTTCCAATATAAAGGAGATATGTGACAAACTGGCGGATG
AGGGATTTGTAGCCTTTGCTCCGGACTTTTACAAGGGTCAATATGCGGACAACCCGGACGATGCGGGAAAACTCATGACC
GAGATGTTTGAAAAGAGAATGGACGAAGTCGATAGAATATTTCAGGCTTCCGTTGAGTTCGTAAAAGAGTGCAGGTACAC
ATACCCTAAAAAAGTGGGGATAACCGGTTTTTGTTGTGGTGGAACACTTGCGATGTACTTTGCCGCAAAGTTTCCCGAAA
TGGTAGACGCAAGCCTGCCATTCTACGGACTACCTCAACTTACTCAGATAAATGCAGAAAATATAAAAGTACCTATTTTC
TTCATACTTGCGGAAAAAGACGAATTCGTTAATAACGACGAAGTAATAGACATAGCAAAGACAGTATGGAAAAATGGAGT
GGACGTTCAGGTAAAAGTATTCTCCGGCGTTACGCATGCGTTCTTAAACGAAAAGAGAGAGGACGTTTACGATCCCAAGC
GTGCCTGTGAAGCGTGGGAACTGGCGGTAAACTTCTTCAAAACATACCTCAAATAA

Upstream 100 bases:

>100_bases
CTGAGAACGTACTTTCCTTCAGGTATTTTTTCCTCACAGGGGAAGGGCAGTAGTGTACTCATATTATAATTGTAGATAAA
CAAAAAGAAGGAGGTGGAAA

Downstream 100 bases:

>100_bases
CCTCCTCCCTCCTCTAAAACTTTTTTCTTTTCCTCCGATAAATATAAATGACAAAACCTTAAAAAGGAGGTAAAAGGGAT
GGCAACGAGGATTAATTACA

Product: hypothetical protein

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MGQMVEFEKNGVKVRGYIATPKWAGPAVLVFHEWWGLESPLSNIKEICDKLADEGFVAFAPDFYKGQYADNPDDAGKLMT
EMFEKRMDEVDRIFQASVEFVKECRYTYPKKVGITGFCCGGTLAMYFAAKFPEMVDASLPFYGLPQLTQINAENIKVPIF
FILAEKDEFVNNDEVIDIAKTVWKNGVDVQVKVFSGVTHAFLNEKREDVYDPKRACEAWELAVNFFKTYLK

Sequences:

>Translated_231_residues
MGQMVEFEKNGVKVRGYIATPKWAGPAVLVFHEWWGLESPLSNIKEICDKLADEGFVAFAPDFYKGQYADNPDDAGKLMT
EMFEKRMDEVDRIFQASVEFVKECRYTYPKKVGITGFCCGGTLAMYFAAKFPEMVDASLPFYGLPQLTQINAENIKVPIF
FILAEKDEFVNNDEVIDIAKTVWKNGVDVQVKVFSGVTHAFLNEKREDVYDPKRACEAWELAVNFFKTYLK
>Mature_230_residues
GQMVEFEKNGVKVRGYIATPKWAGPAVLVFHEWWGLESPLSNIKEICDKLADEGFVAFAPDFYKGQYADNPDDAGKLMTE
MFEKRMDEVDRIFQASVEFVKECRYTYPKKVGITGFCCGGTLAMYFAAKFPEMVDASLPFYGLPQLTQINAENIKVPIFF
ILAEKDEFVNNDEVIDIAKTVWKNGVDVQVKVFSGVTHAFLNEKREDVYDPKRACEAWELAVNFFKTYLK

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family

Homologues:

Organism=Homo sapiens, GI20270371, Length=211, Percent_Identity=30.3317535545024, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI48994982, Length=243, Percent_Identity=23.0452674897119, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DLHH_AQUAE (O67802)

Other databases:

- EMBL:   AE000657
- PIR:   F70471
- RefSeq:   NP_214371.1
- ProteinModelPortal:   O67802
- GeneID:   1193699
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1997
- NMPDR:   fig|224324.1.peg.1386
- HOGENOM:   HBG673102
- OMA:   QEPWKPS
- PhylomeDB:   O67802
- ProtClustDB:   CLSK2299900
- BioCyc:   AAEO224324:AQ_1997-MONOMER
- BRENDA:   3.1.1.45
- InterPro:   IPR002925

Pfam domain/function: PF01738 DLH

EC number: =3.1.1.45

Molecular weight: Translated: 26357; Mature: 26226

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: ACT_SITE 118-118 ACT_SITE 167-167 ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQMVEFEKNGVKVRGYIATPKWAGPAVLVFHEWWGLESPLSNIKEICDKLADEGFVAFA
CCCCEEECCCCEEEEEEEECCCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHCCCEEEEC
PDFYKGQYADNPDDAGKLMTEMFEKRMDEVDRIFQASVEFVKECRYTYPKKVGITGFCCG
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHH
GTLAMYFAAKFPEMVDASLPFYGLPQLTQINAENIKVPIFFILAEKDEFVNNDEVIDIAK
HHHHHHHHHHCHHHHHCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCHHHHHHH
TVWKNGVDVQVKVFSGVTHAFLNEKREDVYDPKRACEAWELAVNFFKTYLK
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GQMVEFEKNGVKVRGYIATPKWAGPAVLVFHEWWGLESPLSNIKEICDKLADEGFVAFA
CCCEEECCCCEEEEEEEECCCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHCCCEEEEC
PDFYKGQYADNPDDAGKLMTEMFEKRMDEVDRIFQASVEFVKECRYTYPKKVGITGFCCG
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHH
GTLAMYFAAKFPEMVDASLPFYGLPQLTQINAENIKVPIFFILAEKDEFVNNDEVIDIAK
HHHHHHHHHHCHHHHHCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCHHHHHHH
TVWKNGVDVQVKVFSGVTHAFLNEKREDVYDPKRACEAWELAVNFFKTYLK
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320