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The map label for this gene is nse [C]

Identifier: 15606538

GI number: 15606538

Start: 950129

End: 951094

Strand: Direct

Name: nse [C]

Synonym: aq_1335

Alternate gene names: 15606538

Gene position: 950129-951094 (Clockwise)

Preceding gene: 15606535

Following gene: 15606545

Centisome position: 61.25

GC content: 42.65

Gene sequence:

>966_bases
ATGAACATCCTAGTAACTGGCTGTGCTGGATTGATAGGGTGGAAGGTATCCGAGAAACTGCTGGAGCATGGACACAGGGT
TATCGGAGTAGATAACTTAAACTCTTACTACGATCCGAGGCTAAAGGAATACAGACTGGAGCAACTAAAAAAATTTGAGA
ACTTTAAGTTTTACAAGGTTAATATAGAAAACAGGGAAGCTTTAAGGATTCTGTTTCAGGAGTTTGAATTTGACGCCGTT
ATAAACGAAGCCGCAAGGGCTGGGGTGAGGTACTCAATACAAAACCCTCACATTTACTTCACAACCAATACTCTAGGAAA
TTTAAACTTATTAGAACTTATGAAAGAATTCGGAGTTAAGAAACTCATTCTCGCCTCTACTTCTTCCCTTTACGCTGGTC
AACCAATGCCTTTTAAAGAAGAACTTCCCGTAAACACACCCATTTCTCCTTACGCTGCAAGCAAGAAGGCGGCAGAAGTT
ACCGCATACACCTACCACTACCTCTACGGGATAGATGTCGTTATCCTGAGGTACTTCACCGTTTACGGACTGGCAGGCAG
ACCCGATATGGCTGTATTTAACTTTATCTACAAAACACTGAAGGGAATTCCTATAAAAGTTTACGGGGATGGAAGTCAGA
AGAGGGATTTCACTTACGTCGACGACGTAGCGGAAGCCACTGTAAAAGCCCTGAACCTCAAGGGATACGAAATAATAAAC
GTGGGAAACAATAAACCGAGGGCTTTGAAGGAGTTAATAGAACTTATAGAAAAGTACACGGGTAAAGAAGTAAAAGTTGA
GTACGGAGATTTTCACAAGGCGGATATGAGGGACACTTGGGCGGACATAACGAAGGCAAAGAGATTACTCGGGTGGGAAC
CTAAAACTTCCCTTGAAGAGGGCGTAAAGAAAACGGTGGAGTGGTTTCTTGAGAACTGGGACTGGGTAAAAGATTTAAGG
GTTTAG

Upstream 100 bases:

>100_bases
GAAGACAGAACACCTCCTGTGATGAAGATGTATTTAGCCATGTAAGAAAATTATCCTACAACGAGGTTTAACGGACAAGT
TTTGAAGTAGAATCTTTGAA

Downstream 100 bases:

>100_bases
TAGAACACCATTCCCGCGTAGGAGACCTTAGAGTCTGTGGGTGCTTCGTGGTAAACGTCGTAATCAAGGAGCTTTCCTTC
TTTAACTCCCAAGTTCTTGA

Product: nucleotide sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNILVTGCAGLIGWKVSEKLLEHGHRVIGVDNLNSYYDPRLKEYRLEQLKKFENFKFYKVNIENREALRILFQEFEFDAV
INEAARAGVRYSIQNPHIYFTTNTLGNLNLLELMKEFGVKKLILASTSSLYAGQPMPFKEELPVNTPISPYAASKKAAEV
TAYTYHYLYGIDVVILRYFTVYGLAGRPDMAVFNFIYKTLKGIPIKVYGDGSQKRDFTYVDDVAEATVKALNLKGYEIIN
VGNNKPRALKELIELIEKYTGKEVKVEYGDFHKADMRDTWADITKAKRLLGWEPKTSLEEGVKKTVEWFLENWDWVKDLR
V

Sequences:

>Translated_321_residues
MNILVTGCAGLIGWKVSEKLLEHGHRVIGVDNLNSYYDPRLKEYRLEQLKKFENFKFYKVNIENREALRILFQEFEFDAV
INEAARAGVRYSIQNPHIYFTTNTLGNLNLLELMKEFGVKKLILASTSSLYAGQPMPFKEELPVNTPISPYAASKKAAEV
TAYTYHYLYGIDVVILRYFTVYGLAGRPDMAVFNFIYKTLKGIPIKVYGDGSQKRDFTYVDDVAEATVKALNLKGYEIIN
VGNNKPRALKELIELIEKYTGKEVKVEYGDFHKADMRDTWADITKAKRLLGWEPKTSLEEGVKKTVEWFLENWDWVKDLR
V
>Mature_321_residues
MNILVTGCAGLIGWKVSEKLLEHGHRVIGVDNLNSYYDPRLKEYRLEQLKKFENFKFYKVNIENREALRILFQEFEFDAV
INEAARAGVRYSIQNPHIYFTTNTLGNLNLLELMKEFGVKKLILASTSSLYAGQPMPFKEELPVNTPISPYAASKKAAEV
TAYTYHYLYGIDVVILRYFTVYGLAGRPDMAVFNFIYKTLKGIPIKVYGDGSQKRDFTYVDDVAEATVKALNLKGYEIIN
VGNNKPRALKELIELIEKYTGKEVKVEYGDFHKADMRDTWADITKAKRLLGWEPKTSLEEGVKKTVEWFLENWDWVKDLR
V

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=326, Percent_Identity=34.0490797546012, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=33.7423312883436, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI56237023, Length=338, Percent_Identity=27.810650887574, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI56118217, Length=338, Percent_Identity=27.810650887574, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI189083684, Length=338, Percent_Identity=27.810650887574, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=28.3185840707965, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=28.6532951289398, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=29.8550724637681, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788365, Length=285, Percent_Identity=27.0175438596491, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788589, Length=349, Percent_Identity=26.0744985673352, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=24.3553008595989, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=348, Percent_Identity=28.1609195402299, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=29.6636085626911, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71982038, Length=350, Percent_Identity=28, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=26.7857142857143, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319493, Length=346, Percent_Identity=29.7687861271676, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356223, Length=319, Percent_Identity=32.2884012539185, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37014; Mature: 37014

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGCAGLIGWKVSEKLLEHGHRVIGVDNLNSYYDPRLKEYRLEQLKKFENFKFYKV
CCEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
NIENREALRILFQEFEFDAVINEAARAGVRYSIQNPHIYFTTNTLGNLNLLELMKEFGVK
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCC
KLILASTSSLYAGQPMPFKEELPVNTPISPYAASKKAAEVTAYTYHYLYGIDVVILRYFT
EEEHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VYGLAGRPDMAVFNFIYKTLKGIPIKVYGDGSQKRDFTYVDDVAEATVKALNLKGYEIIN
HHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
VGNNKPRALKELIELIEKYTGKEVKVEYGDFHKADMRDTWADITKAKRLLGWEPKTSLEE
CCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
GVKKTVEWFLENWDWVKDLRV
HHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MNILVTGCAGLIGWKVSEKLLEHGHRVIGVDNLNSYYDPRLKEYRLEQLKKFENFKFYKV
CCEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
NIENREALRILFQEFEFDAVINEAARAGVRYSIQNPHIYFTTNTLGNLNLLELMKEFGVK
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCC
KLILASTSSLYAGQPMPFKEELPVNTPISPYAASKKAAEVTAYTYHYLYGIDVVILRYFT
EEEHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VYGLAGRPDMAVFNFIYKTLKGIPIKVYGDGSQKRDFTYVDDVAEATVKALNLKGYEIIN
HHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
VGNNKPRALKELIELIEKYTGKEVKVEYGDFHKADMRDTWADITKAKRLLGWEPKTSLEE
CCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
GVKKTVEWFLENWDWVKDLRV
HHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]