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The map label for this gene is gcsH1

Identifier: 15606335

GI number: 15606335

Start: 726937

End: 727368

Strand: Direct

Name: gcsH1

Synonym: aq_1052

Alternate gene names: 15606335

Gene position: 726937-727368 (Clockwise)

Preceding gene: 15606334

Following gene: 15606336

Centisome position: 46.86

GC content: 36.11

Gene sequence:

>432_bases
TTGTGGACTGATAGACTTTACAGAGTCGAACCCTATAGAATGTTATTCCAATGGGTAAAGGATGAAGGAAATGGTATTTA
TTCTGTAGGAATGGCTTCTATTTTAGCTGCTCTAGCGTATCCACTTTACTCGATAAAAATAAAACCTGTTGGCACTAAGC
TGGAGTACGACGAAGCATTAGCAATAATTGAGGCGGGGAAAAGAGTTGCAACATTCCCGACACCTCTATCTGGCATAGTG
GTAGATGTAAATGAAGAAGTGATTAAAAATCCTGAGCTTATAAATAAAAAACCTTACAGTTCTTGGATTGCTAAGCTAAA
AGCTACAAACTTAGAAGAAGTAAAGAACTTACAAAGTGCAAAGGAGATAGTTAAGACGGTGAAAGATTTTATAATTTTGG
AGGATGTAGATTGTTCTATTGTAGAAGAATAG

Upstream 100 bases:

>100_bases
AGCTGTGAAAAGGAGAACGTTTTTAGAATCTCTTCTATTTCTTTTTTTAATAAAAACGGGTTTTAGTATGGAACGAAAGA
TAGACGTTTTAGGATGTACC

Downstream 100 bases:

>100_bases
AAGGGAGAGGTTGGCCGGCTGGTGCCGCCCCGGGACTTCAAATCCCGTGGGAGGTCCCGCAAGGGAGCTCCGGAGGGTTC
GATTCCCTCCCTCTCCCGCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MWTDRLYRVEPYRMLFQWVKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEALAIIEAGKRVATFPTPLSGIV
VDVNEEVIKNPELINKKPYSSWIAKLKATNLEEVKNLQSAKEIVKTVKDFIILEDVDCSIVEE

Sequences:

>Translated_143_residues
MWTDRLYRVEPYRMLFQWVKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEALAIIEAGKRVATFPTPLSGIV
VDVNEEVIKNPELINKKPYSSWIAKLKATNLEEVKNLQSAKEIVKTVKDFIILEDVDCSIVEE
>Mature_143_residues
MWTDRLYRVEPYRMLFQWVKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEALAIIEAGKRVATFPTPLSGIV
VDVNEEVIKNPELINKKPYSSWIAKLKATNLEEVKNLQSAKEIVKTVKDFIILEDVDCSIVEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Escherichia coli, GI1789271, Length=110, Percent_Identity=30, Blast_Score=63, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH1_AQUAE (O67151)

Other databases:

- EMBL:   AE000657
- PIR:   D70390
- RefSeq:   NP_213714.1
- ProteinModelPortal:   O67151
- SMR:   O67151
- GeneID:   1193785
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1052
- NMPDR:   fig|224324.1.peg.729
- HOGENOM:   HBG693789
- OMA:   YSSWIAK
- ProtClustDB:   CLSK2748407
- BioCyc:   AAEO224324:AQ_1052-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 16181; Mature: 16181

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTDRLYRVEPYRMLFQWVKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEAL
CCCCCEEEECHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHH
AIIEAGKRVATFPTPLSGIVVDVNEEVIKNPELINKKPYSSWIAKLKATNLEEVKNLQSA
HHHHCCCEEEECCCCCCCEEEECCHHHHCCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHH
KEIVKTVKDFIILEDVDCSIVEE
HHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MWTDRLYRVEPYRMLFQWVKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEAL
CCCCCEEEECHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHH
AIIEAGKRVATFPTPLSGIVVDVNEEVIKNPELINKKPYSSWIAKLKATNLEEVKNLQSA
HHHHCCCEEEECCCCCCCEEEECCHHHHCCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHH
KEIVKTVKDFIILEDVDCSIVEE
HHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320