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The map label for this gene is nifS1 [H]

Identifier: 15606336

GI number: 15606336

Start: 727480

End: 728592

Strand: Direct

Name: nifS1 [H]

Synonym: aq_1053

Alternate gene names: 15606336

Gene position: 727480-728592 (Clockwise)

Preceding gene: 15606335

Following gene: 15606337

Centisome position: 46.89

GC content: 31.72

Gene sequence:

>1113_bases
ATGATTTACTTTGATAACGCGGCAACTACACCTGTACTTCCAGAAGTAAAAAACTTCCTATGTAAAGCACTAGACATTTA
TGGGAATCCATCAAGTGTACATACACTAGGCAAATTTGCAAGAAGTGAAATAGAAACGGCAAGAAAAACAATTGCGAATT
ATTTGAAAGTAAAACCGGAAAATATTATTTTTAACTCTTGTGCAACTGAAGGAAATAATACGATAATAAGAACGATATTA
AACAATAAAGATAGGGGCAATGTAGTATTATCAGCAATAGAACATAAATCTGTGAAAGAGGCAATAAAATTTTGGGCAAA
TAAAAGTATAGAAATAAGAGAGATTAAAGTAAATAAAAATGGAGTAATTGATTTAGAACACCTTTACCATTTGATTGATA
GTAATACAATTTTAGTTTGCGTTATGTATGTAAGCAACGAATTCGGAACAATTCAGCCTATACAGGAAATAGCTAAAATT
TGCTCCGATAAGGGTGTACCATTGCTAACAGATGCAGTGCAAGCAATAGGAAAAATCCCAATTGAACTAAAAAATATATC
CTATGCGACATTTTCTGGACATAAATTTCACGCTATAAAAGGAAGTGGATTTTTATACATTTCTGACGAAGCTAATTATG
AGCCTCTTATAGTTGGTGGAGGACAAGAAAATGGAAAAAGATCAGGTACAGAAAATGTTGTAGGAATACTTTCGTTAGCT
AAAGCACTTGAAATAATTGTTTCTAACTTTTCGCGCTATCAGGAACAGCTTAAAAAATTAAGAGATCTTTTTGAGAATCT
ATTGCTTGAAGCTTTGCCAGATGCTCAAATTGTAGGAAAAGATGCAGAAAGAAGTCCCTCAATATCTTCTGTTATTATGC
CTAAATTTTTCGGAGCAGAAATAGTTAATAAATTATCAGAAAAGGGAATATATTGCTCGACTGGATCAGCCTGTTTATCA
GGAGAATATGAGCCGAATAAACATATGTTGAAAATGGGGTTTTCTCAAGAAAAAGCTTTAAGAATGGTACGCTTTTCATT
TGGTCTTTTAAACAAAGAGGAAGAAGTAATAGAATGTATAGAAAGAATAAAAGAAATTTATAGATTAAATTAA

Upstream 100 bases:

>100_bases
TGGCCGGCTGGTGCCGCCCCGGGACTTCAAATCCCGTGGGAGGTCCCGCAAGGGAGCTCCGGAGGGTTCGATTCCCTCCC
TCTCCCGCCAATAAGTTGTT

Downstream 100 bases:

>100_bases
AACGTCCATCAACACAATTCTATAAAGGTGTAGAAAAAACAGTTTAGATATTAACTTATCACAGCCTAAAGAAAGGTAAG
AAAGGCTGAAGAAGCAGTTA

Product: FeS cluster formation protein NifS

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MIYFDNAATTPVLPEVKNFLCKALDIYGNPSSVHTLGKFARSEIETARKTIANYLKVKPENIIFNSCATEGNNTIIRTIL
NNKDRGNVVLSAIEHKSVKEAIKFWANKSIEIREIKVNKNGVIDLEHLYHLIDSNTILVCVMYVSNEFGTIQPIQEIAKI
CSDKGVPLLTDAVQAIGKIPIELKNISYATFSGHKFHAIKGSGFLYISDEANYEPLIVGGGQENGKRSGTENVVGILSLA
KALEIIVSNFSRYQEQLKKLRDLFENLLLEALPDAQIVGKDAERSPSISSVIMPKFFGAEIVNKLSEKGIYCSTGSACLS
GEYEPNKHMLKMGFSQEKALRMVRFSFGLLNKEEEVIECIERIKEIYRLN

Sequences:

>Translated_370_residues
MIYFDNAATTPVLPEVKNFLCKALDIYGNPSSVHTLGKFARSEIETARKTIANYLKVKPENIIFNSCATEGNNTIIRTIL
NNKDRGNVVLSAIEHKSVKEAIKFWANKSIEIREIKVNKNGVIDLEHLYHLIDSNTILVCVMYVSNEFGTIQPIQEIAKI
CSDKGVPLLTDAVQAIGKIPIELKNISYATFSGHKFHAIKGSGFLYISDEANYEPLIVGGGQENGKRSGTENVVGILSLA
KALEIIVSNFSRYQEQLKKLRDLFENLLLEALPDAQIVGKDAERSPSISSVIMPKFFGAEIVNKLSEKGIYCSTGSACLS
GEYEPNKHMLKMGFSQEKALRMVRFSFGLLNKEEEVIECIERIKEIYRLN
>Mature_370_residues
MIYFDNAATTPVLPEVKNFLCKALDIYGNPSSVHTLGKFARSEIETARKTIANYLKVKPENIIFNSCATEGNNTIIRTIL
NNKDRGNVVLSAIEHKSVKEAIKFWANKSIEIREIKVNKNGVIDLEHLYHLIDSNTILVCVMYVSNEFGTIQPIQEIAKI
CSDKGVPLLTDAVQAIGKIPIELKNISYATFSGHKFHAIKGSGFLYISDEANYEPLIVGGGQENGKRSGTENVVGILSLA
KALEIIVSNFSRYQEQLKKLRDLFENLLLEALPDAQIVGKDAERSPSISSVIMPKFFGAEIVNKLSEKGIYCSTGSACLS
GEYEPNKHMLKMGFSQEKALRMVRFSFGLLNKEEEVIECIERIKEIYRLN

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=32.8083989501312, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI156713448, Length=410, Percent_Identity=28.0487804878049, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI48994898, Length=379, Percent_Identity=33.245382585752, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1787970, Length=367, Percent_Identity=26.9754768392371, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25143064, Length=378, Percent_Identity=32.8042328042328, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=29.8780487804878, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=372, Percent_Identity=34.1397849462366, Blast_Score=192, Evalue=5e-50,
Organism=Drosophila melanogaster, GI20129463, Length=388, Percent_Identity=33.7628865979381, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41272; Mature: 41272

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNAATTPVLPEVKNFLCKALDIYGNPSSVHTLGKFARSEIETARKTIANYLKVKPE
CEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NIIFNSCATEGNNTIIRTILNNKDRGNVVLSAIEHKSVKEAIKFWANKSIEIREIKVNKN
CEEEECCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GVIDLEHLYHLIDSNTILVCVMYVSNEFGTIQPIQEIAKICSDKGVPLLTDAVQAIGKIP
CCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
IELKNISYATFSGHKFHAIKGSGFLYISDEANYEPLIVGGGQENGKRSGTENVVGILSLA
EEEECCEEEEECCCEEEEEECCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
KALEIIVSNFSRYQEQLKKLRDLFENLLLEALPDAQIVGKDAERSPSISSVIMPKFFGAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHH
IVNKLSEKGIYCSTGSACLSGEYEPNKHMLKMGFSQEKALRMVRFSFGLLNKEEEVIECI
HHHHHHHCCEEEECCCHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
ERIKEIYRLN
HHHHHHHCCC
>Mature Secondary Structure
MIYFDNAATTPVLPEVKNFLCKALDIYGNPSSVHTLGKFARSEIETARKTIANYLKVKPE
CEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NIIFNSCATEGNNTIIRTILNNKDRGNVVLSAIEHKSVKEAIKFWANKSIEIREIKVNKN
CEEEECCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GVIDLEHLYHLIDSNTILVCVMYVSNEFGTIQPIQEIAKICSDKGVPLLTDAVQAIGKIP
CCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
IELKNISYATFSGHKFHAIKGSGFLYISDEANYEPLIVGGGQENGKRSGTENVVGILSLA
EEEECCEEEEECCCEEEEEECCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
KALEIIVSNFSRYQEQLKKLRDLFENLLLEALPDAQIVGKDAERSPSISSVIMPKFFGAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHH
IVNKLSEKGIYCSTGSACLSGEYEPNKHMLKMGFSQEKALRMVRFSFGLLNKEEEVIECI
HHHHHHHCCEEEECCCHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
ERIKEIYRLN
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]