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The map label for this gene is trpS

Identifier: 15603486

GI number: 15603486

Start: 1829092

End: 1830093

Strand: Direct

Name: trpS

Synonym: PM1621

Alternate gene names: 15603486

Gene position: 1829092-1830093 (Clockwise)

Preceding gene: 15603485

Following gene: 15603487

Centisome position: 81.02

GC content: 44.11

Gene sequence:

>1002_bases
ATGAGTAAACCTGTTGTATTAAGTGGCGTACAGCCTTCTGGCGAACTGACGATTGGCAATTATCTTGGCGCATTACGCCA
ATGGGTCAAGATGCAAGATGATTATGAATGCTTATTTTGTATTGTTGATTTACACGCCATTACTGTCCGTCAAGATCCCG
AAAGCTTACGCAAAGCCACCCTTGATGTGTTAGCGTTATATTTAGCCTGTGGCATTGATCCGGAAAAAAGCACTATTTTT
ATCCAATCCCATGTGCCAGAACATACACAACTGGCTTGGGTGCTTAACTGTTACACCTATTTTGGTGAAATGGGGCGCAT
GACCCAATTTAAAGATAAATCGGCGCGCCATGCGGAAAATATCAACGTCGGCTTATTTACTTACCCCGTGTTGATGGCGG
CAGATATTTTGTTATATCAAGCTAACCAAGTGCCTGTAGGGGAAGACCAAAAACAACATCTGGAAATTACTCGTGATATC
GCTAATCGTTTTAACGCGCTATATGGCGATTTATTTGCGGTACCAGAACCTTTTATTCCTAAAGCAGGCGCGCGCGTGAT
GTCATTGCTTGAGCCTGAAAAGAAAATGTCGAAATCAGATGAAAACCGCAATAACGTGATCGGCTTATTAGAAGATCCGA
AAGCTGTCGCGAAGAAAATCAAACGTGCGGTAACTGACTCTGATGAGCCACCAGTAGTCCGTTATGATGTGCAAAACAAA
GCAGGCGTATCGAACTTGTTAGACATTCTTTCTGGTGTCACTGGTAAATCTATCGCGGAATTAGAAACGGAGTTTGAAGG
CAAAATGTATGGGCATTTAAAAGGGACTGTGGCGGATGAAGTCTCCGCGATGCTCACCACCTTACAAGAACGTTTCCACC
ACTTCCGCAACAACGAAGCACTCCTTAACGACATTGCTCGTGAAGGTGCACAAAAAGCACGTGAACGTGCCAAAGCCACC
TTAGATGCCGTTTACCAAGCTGTGGGTTTTGTGTCTTTGTAA

Upstream 100 bases:

>100_bases
CTACGGCTATAACTACAATATTCCCATAGCAGAATCCAAACCAGATTGGGTGTTTGATGATTTTGCACAGATTTTGACGA
TTTTAGAATAAGGAACAACG

Downstream 100 bases:

>100_bases
TCAATGAGCTAATTTTTATCTAAACCGCACATCATGTGCGGTTTTTTTATAAATAAATTGAATGTTATTTATTCACCTCT
TGCATTTCTCTTCAGATATG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSKPVVLSGVQPSGELTIGNYLGALRQWVKMQDDYECLFCIVDLHAITVRQDPESLRKATLDVLALYLACGIDPEKSTIF
IQSHVPEHTQLAWVLNCYTYFGEMGRMTQFKDKSARHAENINVGLFTYPVLMAADILLYQANQVPVGEDQKQHLEITRDI
ANRFNALYGDLFAVPEPFIPKAGARVMSLLEPEKKMSKSDENRNNVIGLLEDPKAVAKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSGVTGKSIAELETEFEGKMYGHLKGTVADEVSAMLTTLQERFHHFRNNEALLNDIAREGAQKARERAKAT
LDAVYQAVGFVSL

Sequences:

>Translated_333_residues
MSKPVVLSGVQPSGELTIGNYLGALRQWVKMQDDYECLFCIVDLHAITVRQDPESLRKATLDVLALYLACGIDPEKSTIF
IQSHVPEHTQLAWVLNCYTYFGEMGRMTQFKDKSARHAENINVGLFTYPVLMAADILLYQANQVPVGEDQKQHLEITRDI
ANRFNALYGDLFAVPEPFIPKAGARVMSLLEPEKKMSKSDENRNNVIGLLEDPKAVAKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSGVTGKSIAELETEFEGKMYGHLKGTVADEVSAMLTTLQERFHHFRNNEALLNDIAREGAQKARERAKAT
LDAVYQAVGFVSL
>Mature_332_residues
SKPVVLSGVQPSGELTIGNYLGALRQWVKMQDDYECLFCIVDLHAITVRQDPESLRKATLDVLALYLACGIDPEKSTIFI
QSHVPEHTQLAWVLNCYTYFGEMGRMTQFKDKSARHAENINVGLFTYPVLMAADILLYQANQVPVGEDQKQHLEITRDIA
NRFNALYGDLFAVPEPFIPKAGARVMSLLEPEKKMSKSDENRNNVIGLLEDPKAVAKKIKRAVTDSDEPPVVRYDVQNKA
GVSNLLDILSGVTGKSIAELETEFEGKMYGHLKGTVADEVSAMLTTLQERFHHFRNNEALLNDIAREGAQKARERAKATL
DAVYQAVGFVSL

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=331, Percent_Identity=42.5981873111783, Blast_Score=274, Evalue=8e-74,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=53.6312849162011, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1789786, Length=332, Percent_Identity=80.421686746988, Blast_Score=578, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71982800, Length=334, Percent_Identity=34.4311377245509, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71982793, Length=334, Percent_Identity=34.4311377245509, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6320474, Length=349, Percent_Identity=40.6876790830946, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24666151, Length=329, Percent_Identity=41.9452887537994, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_PASMU (P57956)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246560.1
- ProteinModelPortal:   P57956
- SMR:   P57956
- GeneID:   1244968
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1621
- NMPDR:   fig|272843.1.peg.1622
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BioCyc:   PMUL272843:PM1621-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37129; Mature: 36998

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVVLSGVQPSGELTIGNYLGALRQWVKMQDDYECLFCIVDLHAITVRQDPESLRKAT
CCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCHHHHHHHH
LDVLALYLACGIDPEKSTIFIQSHVPEHTQLAWVLNCYTYFGEMGRMTQFKDKSARHAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHCC
INVGLFTYPVLMAADILLYQANQVPVGEDQKQHLEITRDIANRFNALYGDLFAVPEPFIP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KAGARVMSLLEPEKKMSKSDENRNNVIGLLEDPKAVAKKIKRAVTDSDEPPVVRYDVQNK
CHHHHHHHHHCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCC
AGVSNLLDILSGVTGKSIAELETEFEGKMYGHLKGTVADEVSAMLTTLQERFHHFRNNEA
CCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHH
LLNDIAREGAQKARERAKATLDAVYQAVGFVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKPVVLSGVQPSGELTIGNYLGALRQWVKMQDDYECLFCIVDLHAITVRQDPESLRKAT
CCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCHHHHHHHH
LDVLALYLACGIDPEKSTIFIQSHVPEHTQLAWVLNCYTYFGEMGRMTQFKDKSARHAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHCC
INVGLFTYPVLMAADILLYQANQVPVGEDQKQHLEITRDIANRFNALYGDLFAVPEPFIP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KAGARVMSLLEPEKKMSKSDENRNNVIGLLEDPKAVAKKIKRAVTDSDEPPVVRYDVQNK
CHHHHHHHHHCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCC
AGVSNLLDILSGVTGKSIAELETEFEGKMYGHLKGTVADEVSAMLTTLQERFHHFRNNEA
CCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHH
LLNDIAREGAQKARERAKATLDAVYQAVGFVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100