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The map label for this gene is gph

Identifier: 15603485

GI number: 15603485

Start: 1828408

End: 1829082

Strand: Direct

Name: gph

Synonym: PM1620

Alternate gene names: 15603485

Gene position: 1828408-1829082 (Clockwise)

Preceding gene: 15603484

Following gene: 15603486

Centisome position: 80.99

GC content: 42.52

Gene sequence:

>675_bases
ATGACACAGTTTAAACTCATTGGTTTTGATCTCGATGGCACCTTAGTCAACAGCTTGCCTGACTTGGCGCTATCGGTAAA
TTCTGCTTTTGCGGAGTTTGATTTACCCCAAGCACCAGAAGACTTGGTGCTGACTTGGATTGGTAACGGTGCGGATATTT
TAATTGCGCGTGCTCTGGCATGGGCGAAAGCTCAAACGGGGAAAACCCTGAATGATGAACAAATTAAAGCGCTTAAGCGA
CGTTTTGGGTTTTATTATGGTGAAAACCTGTGCAACTTGAGTGTACTGTATCCAAATGTCAAATCCACTTTAGAAACGTT
GAAACAAAAAGGCTATCTTTTAGCAGTGGTGACGAATAAGCCAACCAAACATGTGCAACCGGTTTTGCAAGCCTTTGGTA
TTGATCACTTATTCAGCGAACTTTTAGGTGGACAATCTCTGCCCGCGATTAAACCTCACCCCGCACCGCTGTATTATTTA
TGTGGTAAATTTGGTTTATACCCAAAACAGGTGCTCTTTGTGGGGGATTCCAAAAATGATATTTTAGCCGCGCATACTGC
GGGTTGTGCTGTGGTTGGCTTAACCTACGGCTATAACTACAATATTCCCATAGCAGAATCCAAACCAGATTGGGTGTTTG
ATGATTTTGCACAGATTTTGACGATTTTAGAATAA

Upstream 100 bases:

>100_bases
GCAATTTTTGATCAGCCAGATTACCAAAAAGTGATCGATGAAATGCGTCAACAATTGGCGACAGTAGAATAACTAACTTA
ATAGAAAGTGCGGTATAAAA

Downstream 100 bases:

>100_bases
GGAACAACGATGAGTAAACCTGTTGTATTAAGTGGCGTACAGCCTTCTGGCGAACTGACGATTGGCAATTATCTTGGCGC
ATTACGCCAATGGGTCAAGA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTQFKLIGFDLDGTLVNSLPDLALSVNSAFAEFDLPQAPEDLVLTWIGNGADILIARALAWAKAQTGKTLNDEQIKALKR
RFGFYYGENLCNLSVLYPNVKSTLETLKQKGYLLAVVTNKPTKHVQPVLQAFGIDHLFSELLGGQSLPAIKPHPAPLYYL
CGKFGLYPKQVLFVGDSKNDILAAHTAGCAVVGLTYGYNYNIPIAESKPDWVFDDFAQILTILE

Sequences:

>Translated_224_residues
MTQFKLIGFDLDGTLVNSLPDLALSVNSAFAEFDLPQAPEDLVLTWIGNGADILIARALAWAKAQTGKTLNDEQIKALKR
RFGFYYGENLCNLSVLYPNVKSTLETLKQKGYLLAVVTNKPTKHVQPVLQAFGIDHLFSELLGGQSLPAIKPHPAPLYYL
CGKFGLYPKQVLFVGDSKNDILAAHTAGCAVVGLTYGYNYNIPIAESKPDWVFDDFAQILTILE
>Mature_223_residues
TQFKLIGFDLDGTLVNSLPDLALSVNSAFAEFDLPQAPEDLVLTWIGNGADILIARALAWAKAQTGKTLNDEQIKALKRR
FGFYYGENLCNLSVLYPNVKSTLETLKQKGYLLAVVTNKPTKHVQPVLQAFGIDHLFSELLGGQSLPAIKPHPAPLYYLC
GKFGLYPKQVLFVGDSKNDILAAHTAGCAVVGLTYGYNYNIPIAESKPDWVFDDFAQILTILE

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=230, Percent_Identity=45.6521739130435, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_PASMU (Q9CKJ5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246559.1
- ProteinModelPortal:   Q9CKJ5
- SMR:   Q9CKJ5
- GeneID:   1244967
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1620
- NMPDR:   fig|272843.1.peg.1621
- HOGENOM:   HBG742904
- OMA:   YNYGESI
- ProtClustDB:   PRK13222
- BioCyc:   PMUL272843:PM1620-MONOMER
- BRENDA:   3.1.3.18
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 24623; Mature: 24491

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS01228 COF_1

Important sites: ACT_SITE 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFKLIGFDLDGTLVNSLPDLALSVNSAFAEFDLPQAPEDLVLTWIGNGADILIARALA
CCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
WAKAQTGKTLNDEQIKALKRRFGFYYGENLCNLSVLYPNVKSTLETLKQKGYLLAVVTNK
HHHHHCCCCCCHHHHHHHHHHHCHHHCCCCEEEEEECCCHHHHHHHHHHCCEEEEEEECC
PTKHVQPVLQAFGIDHLFSELLGGQSLPAIKPHPAPLYYLCGKFGLYPKQVLFVGDSKND
CHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCCCEEEEEEECCCCC
ILAAHTAGCAVVGLTYGYNYNIPIAESKPDWVFDDFAQILTILE
EEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TQFKLIGFDLDGTLVNSLPDLALSVNSAFAEFDLPQAPEDLVLTWIGNGADILIARALA
CEEEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
WAKAQTGKTLNDEQIKALKRRFGFYYGENLCNLSVLYPNVKSTLETLKQKGYLLAVVTNK
HHHHHCCCCCCHHHHHHHHHHHCHHHCCCCEEEEEECCCHHHHHHHHHHCCEEEEEEECC
PTKHVQPVLQAFGIDHLFSELLGGQSLPAIKPHPAPLYYLCGKFGLYPKQVLFVGDSKND
CHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCCCEEEEEEECCCCC
ILAAHTAGCAVVGLTYGYNYNIPIAESKPDWVFDDFAQILTILE
EEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100