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The map label for this gene is rbsB_2 [H]

Identifier: 15603242

GI number: 15603242

Start: 1568990

End: 1569919

Strand: Reverse

Name: rbsB_2 [H]

Synonym: PM1377

Alternate gene names: 15603242

Gene position: 1569919-1568990 (Counterclockwise)

Preceding gene: 15603243

Following gene: 15603240

Centisome position: 69.54

GC content: 43.44

Gene sequence:

>930_bases
ATGAAATTAAAAACATTAGCAGTATCTTTAATGTTAGCCATGGCACCTTTTGCACAAGCGAAAGAATTAAAATCGATCGG
TGTGACCGTTGGTGATCTCGCTAACCCATTCTTCGTACAAATTGCGAAAGGGGCCGAGTTAAAAGCAAAAGAGCTTGCTG
GCGACAAAGTAAACGTTGTTTCTGTATCCAGTGGTTACGATTTAGGTCAACAAGTGGCACAAATCGATAACTTCATCGCG
GCAAAAACCGACATGATTATCCTTAACGCAGCAGATTCTAAAGGGATTGGACCTGCCGTAGAGCGTGCACGTAAAGCTGG
TATTGTGGTGGTGGCAGTTGACGTGGCAGCGGATGGCGCGGACGCAACAGTCACTTCAAATAACTACCAAGCGGGTGAAA
TTGCTTGCCAAACTATCGTTGAAAAACTTAACGGTAAAGGGAATGTGGTGATCATCAATGGACCACCAGTATCTGCAGTA
CAAGATCGTGTACAAGGTTGCCAAGATGTATTTGCTAAACACGCGGACATCAAAGTGTTATCACATAACCAAAACGCGAA
AGGTAGCCGTGAAGGTGGTTTAGAAGTGATGACTTCATTATTAATTGCACATCCGAAAATTGATGCGGTGTTCGCCATCA
ATGACCCAACGGCGATTGGTGCAGATTTAGCTGCAAAACAAGCACAACGTAATGAGTTTTTCATTATGGGTGTGGACGGC
TCGCCAGATGGTGAAGAAGCCTTGAAATTATCAGATAGCTTATTCCGTGGTACACCAGCACAAGATCCACAAGTGATGGC
AGCGGAAGCGGTGAAAATTGGTTACGATATTTTACAAGGCAAACCAGCCCCGAAAGATCCCGTGTTAATTCCTGTACATT
TAATTACACGTGATAACATTAAAGATTATAAAGGTTGGACTGTGAAGTAA

Upstream 100 bases:

>100_bases
GCAGCTCGTTATTAAAGGTGGGGTAATCATCGTGGCAGTATTGATCGACAAGTACCGTACCCGTTCAGCTTAAACAACAT
CCAATATAGGAGACGTTATA

Downstream 100 bases:

>100_bases
TTACCTGATTCCCCCTCCTGAGTTTGGGAGGGGGCTTATTATTTGTGGTTAAACATGCGACATTAACAGCGCTCAACCGC
GACGGCAAATAAATAACCCA

Product: hypothetical protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKLKTLAVSLMLAMAPFAQAKELKSIGVTVGDLANPFFVQIAKGAELKAKELAGDKVNVVSVSSGYDLGQQVAQIDNFIA
AKTDMIILNAADSKGIGPAVERARKAGIVVVAVDVAADGADATVTSNNYQAGEIACQTIVEKLNGKGNVVIINGPPVSAV
QDRVQGCQDVFAKHADIKVLSHNQNAKGSREGGLEVMTSLLIAHPKIDAVFAINDPTAIGADLAAKQAQRNEFFIMGVDG
SPDGEEALKLSDSLFRGTPAQDPQVMAAEAVKIGYDILQGKPAPKDPVLIPVHLITRDNIKDYKGWTVK

Sequences:

>Translated_309_residues
MKLKTLAVSLMLAMAPFAQAKELKSIGVTVGDLANPFFVQIAKGAELKAKELAGDKVNVVSVSSGYDLGQQVAQIDNFIA
AKTDMIILNAADSKGIGPAVERARKAGIVVVAVDVAADGADATVTSNNYQAGEIACQTIVEKLNGKGNVVIINGPPVSAV
QDRVQGCQDVFAKHADIKVLSHNQNAKGSREGGLEVMTSLLIAHPKIDAVFAINDPTAIGADLAAKQAQRNEFFIMGVDG
SPDGEEALKLSDSLFRGTPAQDPQVMAAEAVKIGYDILQGKPAPKDPVLIPVHLITRDNIKDYKGWTVK
>Mature_309_residues
MKLKTLAVSLMLAMAPFAQAKELKSIGVTVGDLANPFFVQIAKGAELKAKELAGDKVNVVSVSSGYDLGQQVAQIDNFIA
AKTDMIILNAADSKGIGPAVERARKAGIVVVAVDVAADGADATVTSNNYQAGEIACQTIVEKLNGKGNVVIINGPPVSAV
QDRVQGCQDVFAKHADIKVLSHNQNAKGSREGGLEVMTSLLIAHPKIDAVFAINDPTAIGADLAAKQAQRNEFFIMGVDG
SPDGEEALKLSDSLFRGTPAQDPQVMAAEAVKIGYDILQGKPAPKDPVLIPVHLITRDNIKDYKGWTVK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=291, Percent_Identity=33.6769759450172, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790526, Length=292, Percent_Identity=29.4520547945205, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32492; Mature: 32492

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTLAVSLMLAMAPFAQAKELKSIGVTVGDLANPFFVQIAKGAELKAKELAGDKVNVV
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCHHHHCCCEEEEE
SVSSGYDLGQQVAQIDNFIAAKTDMIILNAADSKGIGPAVERARKAGIVVVAVDVAADGA
EECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEEEEEECCCC
DATVTSNNYQAGEIACQTIVEKLNGKGNVVIINGPPVSAVQDRVQGCQDVFAKHADIKVL
CCEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEE
SHNQNAKGSREGGLEVMTSLLIAHPKIDAVFAINDPTAIGADLAAKQAQRNEFFIMGVDG
ECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECCC
SPDGEEALKLSDSLFRGTPAQDPQVMAAEAVKIGYDILQGKPAPKDPVLIPVHLITRDNI
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
KDYKGWTVK
CCCCCCCCC
>Mature Secondary Structure
MKLKTLAVSLMLAMAPFAQAKELKSIGVTVGDLANPFFVQIAKGAELKAKELAGDKVNVV
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCHHHHCCCEEEEE
SVSSGYDLGQQVAQIDNFIAAKTDMIILNAADSKGIGPAVERARKAGIVVVAVDVAADGA
EECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEEEEEECCCC
DATVTSNNYQAGEIACQTIVEKLNGKGNVVIINGPPVSAVQDRVQGCQDVFAKHADIKVL
CCEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEE
SHNQNAKGSREGGLEVMTSLLIAHPKIDAVFAINDPTAIGADLAAKQAQRNEFFIMGVDG
ECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEECCC
SPDGEEALKLSDSLFRGTPAQDPQVMAAEAVKIGYDILQGKPAPKDPVLIPVHLITRDNI
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
KDYKGWTVK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]