Click here to switch to the map view.

The map label for this gene is mak [H]

Identifier: 15603240

GI number: 15603240

Start: 1567196

End: 1568107

Strand: Reverse

Name: mak [H]

Synonym: PM1375

Alternate gene names: 15603240

Gene position: 1568107-1567196 (Counterclockwise)

Preceding gene: 15603242

Following gene: 15603239

Centisome position: 69.46

GC content: 46.49

Gene sequence:

>912_bases
ATGCGTATTGGCATCGATCTGGGTGGCACGAAAATTGAAGTGATCGCCCTTTCTAATGAAGGCAAAGAGTTATTCAGAAA
ACGAGTACCTACACCAAGAGGGTCTTATGAAGCAACATTAGCTGCAATTAAAGGGCTGGTAGATGATGCGGAACAAGCAA
CGGGACAAACAGGTACGGTGGGTATTGGTATTCCGGGGACGATTTCGCCGTTTAGCCATAAAGTGAAAAATGCTAATTCC
GTGTGGTTAAATGGTCAACCATTAGACAAAGATCTTTGTCGCTTACTGGGTCGCGAAGTCCGTATTGCCAATGACGCAAA
TTGCATGACAGTATCTGAAGCCACAGACGGTGCAGGGGCAGGAAGTGCGGTCGTGTTGGCGTTAATTTTAGGAACAGGAT
GCGGTTCAGGCATTGTGATTCATGGTAAACCACATCATGGTGGTAACGGTATCGGGGGAGAATGGGGACACAATCCTTTG
CCTTGGATGGATGATGAAGAAAAAGCCGTTGCGCAACAAACACCATGTTATTGTGGTAAACACGGTTGTATTGAACAATT
TGTGTCAGGCACAGGGCTGTGTGATGATTATGAGCGTCGTTCGGGAAAACGTTTGAAAGGAGATGAGATTCTTGCTTTAG
CTGAGCAAGGCGATGCGCTAGCCGAACAATCCTTCCAAGCTTATGAACGTCGCTTGGCGAAAGCGTTGTCCTCTTATGTC
AATATGCTCGATCCTGATGTGATTGTCTTCGCAGGCGGTGTGTGTAATGTTGATAGACTTTACACCAATGTGCCTAAGCT
GATGCCACAGTATATTTTTGGTCATGAGTTCCATACTCCTATTCGCAAGGCATTACATGGTGATTCCAGTGGCGTGCGTG
GTGCGGCATGGCTATGGCCACTGGAAAAATAA

Upstream 100 bases:

>100_bases
TTGTATATCCATGCCTTAAATAGATTATTTTTTAAGTATATATAATTTCTAACAGAAAGAGTATTCATCAATAAACAAAA
TCTAAAACGATGAGGTAGTT

Downstream 100 bases:

>100_bases
AATTTCGTTAAATTGACCGCACTTTGATGGGGTTTCTTTAAGGGGGATAAGCGATGAAACGTTCTGAAATTAACCAAGTA
TTAGGCGAATTTAAGGCATT

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRIGIDLGGTKIEVIALSNEGKELFRKRVPTPRGSYEATLAAIKGLVDDAEQATGQTGTVGIGIPGTISPFSHKVKNANS
VWLNGQPLDKDLCRLLGREVRIANDANCMTVSEATDGAGAGSAVVLALILGTGCGSGIVIHGKPHHGGNGIGGEWGHNPL
PWMDDEEKAVAQQTPCYCGKHGCIEQFVSGTGLCDDYERRSGKRLKGDEILALAEQGDALAEQSFQAYERRLAKALSSYV
NMLDPDVIVFAGGVCNVDRLYTNVPKLMPQYIFGHEFHTPIRKALHGDSSGVRGAAWLWPLEK

Sequences:

>Translated_303_residues
MRIGIDLGGTKIEVIALSNEGKELFRKRVPTPRGSYEATLAAIKGLVDDAEQATGQTGTVGIGIPGTISPFSHKVKNANS
VWLNGQPLDKDLCRLLGREVRIANDANCMTVSEATDGAGAGSAVVLALILGTGCGSGIVIHGKPHHGGNGIGGEWGHNPL
PWMDDEEKAVAQQTPCYCGKHGCIEQFVSGTGLCDDYERRSGKRLKGDEILALAEQGDALAEQSFQAYERRLAKALSSYV
NMLDPDVIVFAGGVCNVDRLYTNVPKLMPQYIFGHEFHTPIRKALHGDSSGVRGAAWLWPLEK
>Mature_303_residues
MRIGIDLGGTKIEVIALSNEGKELFRKRVPTPRGSYEATLAAIKGLVDDAEQATGQTGTVGIGIPGTISPFSHKVKNANS
VWLNGQPLDKDLCRLLGREVRIANDANCMTVSEATDGAGAGSAVVLALILGTGCGSGIVIHGKPHHGGNGIGGEWGHNPL
PWMDDEEKAVAQQTPCYCGKHGCIEQFVSGTGLCDDYERRSGKRLKGDEILALAEQGDALAEQSFQAYERRLAKALSSYV
NMLDPDVIVFAGGVCNVDRLYTNVPKLMPQYIFGHEFHTPIRKALHGDSSGVRGAAWLWPLEK

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=63.9072847682119, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=40.3973509933775, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1790522, Length=303, Percent_Identity=25.4125412541254, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=22.5454545454545, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787878, Length=211, Percent_Identity=25.5924170616114, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32405; Mature: 32405

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKIEVIALSNEGKELFRKRVPTPRGSYEATLAAIKGLVDDAEQATGQTGTV
CEEEEECCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
GIGIPGTISPFSHKVKNANSVWLNGQPLDKDLCRLLGREVRIANDANCMTVSEATDGAGA
EECCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHCCCEEEECCCCEEEEECCCCCCCC
GSAVVLALILGTGCGSGIVIHGKPHHGGNGIGGEWGHNPLPWMDDEEKAVAQQTPCYCGK
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
HGCIEQFVSGTGLCDDYERRSGKRLKGDEILALAEQGDALAEQSFQAYERRLAKALSSYV
HHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHH
NMLDPDVIVFAGGVCNVDRLYTNVPKLMPQYIFGHEFHTPIRKALHGDSSGVRGAAWLWP
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECC
LEK
CCC
>Mature Secondary Structure
MRIGIDLGGTKIEVIALSNEGKELFRKRVPTPRGSYEATLAAIKGLVDDAEQATGQTGTV
CEEEEECCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
GIGIPGTISPFSHKVKNANSVWLNGQPLDKDLCRLLGREVRIANDANCMTVSEATDGAGA
EECCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHCCCEEEECCCCEEEEECCCCCCCC
GSAVVLALILGTGCGSGIVIHGKPHHGGNGIGGEWGHNPLPWMDDEEKAVAQQTPCYCGK
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
HGCIEQFVSGTGLCDDYERRSGKRLKGDEILALAEQGDALAEQSFQAYERRLAKALSSYV
HHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHH
NMLDPDVIVFAGGVCNVDRLYTNVPKLMPQYIFGHEFHTPIRKALHGDSSGVRGAAWLWP
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECC
LEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]