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The map label for this gene is trmB

Identifier: 15603182

GI number: 15603182

Start: 1509644

End: 1510411

Strand: Reverse

Name: trmB

Synonym: PM1317

Alternate gene names: 15603182

Gene position: 1510411-1509644 (Counterclockwise)

Preceding gene: 15603187

Following gene: 15603181

Centisome position: 66.91

GC content: 42.71

Gene sequence:

>768_bases
ATGTCATTTAATAATCAAGAATCAGAGAATTTACCAACCTTTGCTGATCAAAAACGTAAAACTGTGGAAGTTGCCGAATT
TACCGCAGAGGGGCGCTATAAACGCAAAGTTCGCAGTTTCGTATTACGTACTGGGCGTTTGAGTGAATTTCAAAAAAATG
CCATGAATCAACATTGGGGATTCTACGGTTTAGAACATCAAAACCAGCCCTTTGATTTTCCTGCTATTTATGGAAACAAT
AATCCTGTGGTACTCGAAATTGGCTTTGGTATGGGACACTCTTTAGTTGACATGGCACTGCAAAACCCTGAGCGCAATTA
TTTAGGGATCGAAGTTCACACGCCTGGTGTCGGGGCTTGTATCGCCTATGCGGTGGAAAAAGGGGTAAAAAATTTACGTA
TCATTTGCCATGATGCCACCGAAATTTTACGTGATTGCATTGCGGACCACAGCTTAGGTGGTTTGCAATTATTCTTCCCG
GATCCATGGCATAAAGCAAAGCACCACAAACGCCGCATCGTCCAACCGCAATTTGTGAATACCATTACACAAAAGTTGAC
AACAGGTGGTTTTATTCACATGGCCACCGACTGGGAAAACTATGCAGAGCATATGTTAGCCGTGTTGCAACAAGCCGAAG
GTTTACAAAATACATCAGCGACCCATGATTACATTCCACGCCCGGATTTCCGTCCATTAACTAAATTTGAGCAACGTGGA
CATCGTTTAGGACATGGCGTGTGGGATCTCTACTACATCAAAAAATAA

Upstream 100 bases:

>100_bases
ATAAAATGTCAATAAATACACAAGCCTGCTCGACAAGCTTGGTGTTTTCCAACAAAAACCGTATAATTCGCGGCAAATTA
GCCTCATTATAAACAAAAAT

Downstream 100 bases:

>100_bases
AAAAACAAGCCCGCACTTTTTGAACAAAAGTCTGGCATAATGTAAGACAAATTTTATCAGCCAACAGAGAGGAGAATCGA
ACATGGCGATTAAACGTAAC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSFNNQESENLPTFADQKRKTVEVAEFTAEGRYKRKVRSFVLRTGRLSEFQKNAMNQHWGFYGLEHQNQPFDFPAIYGNN
NPVVLEIGFGMGHSLVDMALQNPERNYLGIEVHTPGVGACIAYAVEKGVKNLRIICHDATEILRDCIADHSLGGLQLFFP
DPWHKAKHHKRRIVQPQFVNTITQKLTTGGFIHMATDWENYAEHMLAVLQQAEGLQNTSATHDYIPRPDFRPLTKFEQRG
HRLGHGVWDLYYIKK

Sequences:

>Translated_255_residues
MSFNNQESENLPTFADQKRKTVEVAEFTAEGRYKRKVRSFVLRTGRLSEFQKNAMNQHWGFYGLEHQNQPFDFPAIYGNN
NPVVLEIGFGMGHSLVDMALQNPERNYLGIEVHTPGVGACIAYAVEKGVKNLRIICHDATEILRDCIADHSLGGLQLFFP
DPWHKAKHHKRRIVQPQFVNTITQKLTTGGFIHMATDWENYAEHMLAVLQQAEGLQNTSATHDYIPRPDFRPLTKFEQRG
HRLGHGVWDLYYIKK
>Mature_254_residues
SFNNQESENLPTFADQKRKTVEVAEFTAEGRYKRKVRSFVLRTGRLSEFQKNAMNQHWGFYGLEHQNQPFDFPAIYGNNN
PVVLEIGFGMGHSLVDMALQNPERNYLGIEVHTPGVGACIAYAVEKGVKNLRIICHDATEILRDCIADHSLGGLQLFFPD
PWHKAKHHKRRIVQPQFVNTITQKLTTGGFIHMATDWENYAEHMLAVLQQAEGLQNTSATHDYIPRPDFRPLTKFEQRGH
RLGHGVWDLYYIKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=58.5903083700441, Blast_Score=292, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PASMU (Q9CLC2)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246255.1
- ProteinModelPortal:   Q9CLC2
- GeneID:   1244664
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1317
- NMPDR:   fig|272843.1.peg.1318
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   PMUL272843:PM1317-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29320; Mature: 29188

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: BINDING 86-86 BINDING 111-111 BINDING 138-138 BINDING 161-161 BINDING 165-165 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNNQESENLPTFADQKRKTVEVAEFTAEGRYKRKVRSFVLRTGRLSEFQKNAMNQHWG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
FYGLEHQNQPFDFPAIYGNNNPVVLEIGFGMGHSLVDMALQNPERNYLGIEVHTPGVGAC
EECCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHH
IAYAVEKGVKNLRIICHDATEILRDCIADHSLGGLQLFFPDPWHKAKHHKRRIVQPQFVN
HHHHHHHCCHHEEEEECCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHH
TITQKLTTGGFIHMATDWENYAEHMLAVLQQAEGLQNTSATHDYIPRPDFRPLTKFEQRG
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCC
HRLGHGVWDLYYIKK
HHHCCCCEEEEEECC
>Mature Secondary Structure 
SFNNQESENLPTFADQKRKTVEVAEFTAEGRYKRKVRSFVLRTGRLSEFQKNAMNQHWG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
FYGLEHQNQPFDFPAIYGNNNPVVLEIGFGMGHSLVDMALQNPERNYLGIEVHTPGVGAC
EECCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHH
IAYAVEKGVKNLRIICHDATEILRDCIADHSLGGLQLFFPDPWHKAKHHKRRIVQPQFVN
HHHHHHHCCHHEEEEECCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHH
TITQKLTTGGFIHMATDWENYAEHMLAVLQQAEGLQNTSATHDYIPRPDFRPLTKFEQRG
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCC
HRLGHGVWDLYYIKK
HHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100