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The map label for this gene is tcmP [H]

Identifier: 15603187

GI number: 15603187

Start: 1514139

End: 1514969

Strand: Reverse

Name: tcmP [H]

Synonym: PM1322

Alternate gene names: 15603187

Gene position: 1514969-1514139 (Counterclockwise)

Preceding gene: 15603188

Following gene: 15603182

Centisome position: 67.11

GC content: 39.23

Gene sequence:

>831_bases
ATGTCTGACAAAATTTCACCCAATAAGATATCTGCGCTTTCTTCTACTTTATTAATCACTCTTTGGGCAAAAGCAGTTGA
ATATGATAAAGCCAATCCATTACTGAAAGATCGCGAAGCAGCAAGAATGAAAAAACAGATTGACTATGACTTTCAAAAGT
TTGAATCTGCTCATTTATCACAAGTGGGATGTTGTGGACGCGCAAAATTATTTGATCAAGAAAGCTTAAAATTTCTTTCA
CAGCACCAAGACGCGGTTGTTGTGCAGCTTGGTGCGGGCTTAGATGCACGCTTTGAACGCTTAGGCAAACCACAAGTCAG
TGCGTGGTATGATTTAGACTTACCTGAAGTCATCAATATACGTCGCCAACTTTTACCAGAAACGAGTAATCATTATTTGG
CTGACTCACTTTTCAATACAGATTGGATGAAAACAGTTAGTCAACATAACAAACCCGTTTTATTAATTCTTGAAGGCGTA
TTGATGTTTTTTCCTAAAGAACAAGTCAAACAGTTTATTGCCTCTGTGGCTGAAAACTTACCTAACAGCACAATGATTTT
CGATATTGTGCCCCCAATGGCAGTCGGTCGTAGTAAATACCACGATGCACTCAAAAAAATAGACAGTCAAGAACGCCCTG
AATTTTCATGGACAATACAAGAGATCAAAGAAATTGAAACATGGCATGCGGCAATTAAATTACGCTCAGTCCATTACATA
AGCGATGTCTGTCGGGATCGCTATCCTTGCTGGACAAGATTACTTTACGCTACTCGCTGGGGAAAACACAATTTAGACCC
GCGTGTCGTCACAATAAGCTTTGTTACTTAA

Upstream 100 bases:

>100_bases
GAGTCTTTTTACTCCTTCAATAAAAGTATTGAATACGATGTCACCTTTTTCTATGATATCTTGATTATTTGAGAATTATT
ATCATTAAATAGGAGGTGTT

Downstream 100 bases:

>100_bases
CAAGCAAAAAGAACTTGCTCTGCTCCTGAATAACCAATCTAGCAAAAGCGAAACCGAGCATACATTCTCTCCACCAAAAG
CAACAACGTTTTTTGGTAAC

Product: TcmP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSDKISPNKISALSSTLLITLWAKAVEYDKANPLLKDREAARMKKQIDYDFQKFESAHLSQVGCCGRAKLFDQESLKFLS
QHQDAVVVQLGAGLDARFERLGKPQVSAWYDLDLPEVINIRRQLLPETSNHYLADSLFNTDWMKTVSQHNKPVLLILEGV
LMFFPKEQVKQFIASVAENLPNSTMIFDIVPPMAVGRSKYHDALKKIDSQERPEFSWTIQEIKEIETWHAAIKLRSVHYI
SDVCRDRYPCWTRLLYATRWGKHNLDPRVVTISFVT

Sequences:

>Translated_276_residues
MSDKISPNKISALSSTLLITLWAKAVEYDKANPLLKDREAARMKKQIDYDFQKFESAHLSQVGCCGRAKLFDQESLKFLS
QHQDAVVVQLGAGLDARFERLGKPQVSAWYDLDLPEVINIRRQLLPETSNHYLADSLFNTDWMKTVSQHNKPVLLILEGV
LMFFPKEQVKQFIASVAENLPNSTMIFDIVPPMAVGRSKYHDALKKIDSQERPEFSWTIQEIKEIETWHAAIKLRSVHYI
SDVCRDRYPCWTRLLYATRWGKHNLDPRVVTISFVT
>Mature_275_residues
SDKISPNKISALSSTLLITLWAKAVEYDKANPLLKDREAARMKKQIDYDFQKFESAHLSQVGCCGRAKLFDQESLKFLSQ
HQDAVVVQLGAGLDARFERLGKPQVSAWYDLDLPEVINIRRQLLPETSNHYLADSLFNTDWMKTVSQHNKPVLLILEGVL
MFFPKEQVKQFIASVAENLPNSTMIFDIVPPMAVGRSKYHDALKKIDSQERPEFSWTIQEIKEIETWHAAIKLRSVHYIS
DVCRDRYPCWTRLLYATRWGKHNLDPRVVTISFVT

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: COG3315

COG function: function code Q; O-Methyltransferase involved in polyketide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 31825; Mature: 31694

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKISPNKISALSSTLLITLWAKAVEYDKANPLLKDREAARMKKQIDYDFQKFESAHLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
QVGCCGRAKLFDQESLKFLSQHQDAVVVQLGAGLDARFERLGKPQVSAWYDLDLPEVINI
HHCCCCCHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCEEECCCCHHHHHH
RRQLLPETSNHYLADSLFNTDWMKTVSQHNKPVLLILEGVLMFFPKEQVKQFIASVAENL
HHHHCCCCCCCHHHHHHHCHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHC
PNSTMIFDIVPPMAVGRSKYHDALKKIDSQERPEFSWTIQEIKEIETWHAAIKLRSVHYI
CCCCEEEECCCCHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDVCRDRYPCWTRLLYATRWGKHNLDPRVVTISFVT
HHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
SDKISPNKISALSSTLLITLWAKAVEYDKANPLLKDREAARMKKQIDYDFQKFESAHLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
QVGCCGRAKLFDQESLKFLSQHQDAVVVQLGAGLDARFERLGKPQVSAWYDLDLPEVINI
HHCCCCCHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCEEECCCCHHHHHH
RRQLLPETSNHYLADSLFNTDWMKTVSQHNKPVLLILEGVLMFFPKEQVKQFIASVAENL
HHHHCCCCCCCHHHHHHHCHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHC
PNSTMIFDIVPPMAVGRSKYHDALKKIDSQERPEFSWTIQEIKEIETWHAAIKLRSVHYI
CCCCEEEECCCCHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDVCRDRYPCWTRLLYATRWGKHNLDPRVVTISFVT
HHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]