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The map label for this gene is ponA [H]

Identifier: 15603095

GI number: 15603095

Start: 1420199

End: 1422760

Strand: Direct

Name: ponA [H]

Synonym: PM1230

Alternate gene names: 15603095

Gene position: 1420199-1422760 (Clockwise)

Preceding gene: 15603086

Following gene: 15603097

Centisome position: 62.91

GC content: 42.7

Gene sequence:

>2562_bases
ATGCGGATCGCAAAACTAATACTCAGTACCCTGCTCACGCTCATCGTACTAGGCTGTCTCGCGCTAGGTGCGATCTATGT
TTATTTAAAGGCGGACTTACCGGATGTAGAAAGCCTAAAAACAGTCGAATTACAACAGCCAATGCAAATTTATACGGCTG
ATGGCAAACTGATTGGCGAAGTGGGAGAACAACGTCGGATTCCAGTGCCACTGGATAAAATTCCAAAACAACTGATTCAT
GCTGTTTTAGCAACAGAAGATAACCGTTTCTATGAACATCATGGGCTCGATCCTATTGGCATTGCGCGTGCTATTATGGT
CGCGGTTACGAAGAAAGGCGTTTCACAAGGCGCCAGTACAATTACTCAACAATTAGCACGAAATTTTTTCTTAACCCCTG
AAAAATCGCTTGAACGCAAAGCTAAAGAAGCCGTTTTAGCCATTGATATCGAAAATACACTAAGTAAAAATGAGATTTTA
GAACTCTATCTGAACAAAATTTATTTAGGCTATCGTTCTTATGGCGTTGCCGCTGCCGCCAAAACCTATTTTGGCAAAGA
GCTCGATCAACTAAGCTTGTCCGAAATTGCGATTATCGCTGGCTTACCTAAAGCACCTTCCACAATGAACCCGATTTATT
CATTAAAACGGGCGCAAGAACGCCGTAATATTGTCTTAGGACGGATGTTAACCACTAAATACATCACACAAGAAGAATAT
GACGCGGCAATCAAAGAACCTATCGTCGCGCGCTACCATGGTGCACAAATTGATTTCCGTGCAGACTATGTCACTGAAAT
GGCACGTCAAGAAATGGTCAGACGTTTTGGTGAAGAAAAAGCCTACACTAGCGGTTTTAAAGTTTATACTACGGTGCTTT
CGAAGGATCAGGCTGAAGCGCAAAAAGCCTTACGTAATAACTTAATTGATTACGATATGCGCCATGGTTATCGTGGCGGT
GCGCCACTTTGGCAAAAAGACGAAACGGTGTGGGACAATGATCGTATCATAGGTTTCTTAAAAAAATTACCACATTCTGA
ACCGCTCTTACCGGCTGCCGTCATGAATATCAGCAAAACCGGAGCAGAACTTCTTCTAGCCTCAGGTGAACGCATGACCT
TATCACACAATGCTATGCGTTGGGCGGGTAAAAAAACACCAAAAGTAGGCGAACAAATTTGGATTCGTCAAAAAGAGAAA
GGGGAATGGATTCTGGCTCAAGTGCCGGAAGTCAACTCTGCCTTGGTATCCTTAAATAGTGATAATGGTGCAATTGAAGC
CTTAGTTGGTGGATTTAGTTTCGAACAAAGCAAATTTAACCGTGCTACACAATCTTTAGTCCAAGTGGGCTCTTCAATTA
AACCGTTTATTTATGCGGCCGCATTAGAAAAAGGGTTAACGCTTTCAAGCGTCTTACAAGACTCGCCTCTTGTCATACAA
AAAGCAGGACAAAAACCTTGGCAGCCAAAAAACTCACCAAATCGATTTGATGGACCAATGCGCTTGCGGGTTGGCTTAGG
ATTATCAAAAAATATGATTGCGATTCGTGCGATGCAAATGGCGGGCATTGATTTTACAGCAGAATTCTTACAACGCTTTG
GTTTTAAACGCGATCAATACTTAGCCAGTGAAGCCCTTGCCTTAGGTGCGGCCTCTTTTACTCCACTTGAAATGGCAAGA
GGCTACGCGGTCTTTGACAATGGCGGTTTCTTAGTTGATCCCTTTATTATTAATCGTATTTTAGACAATACGGGCAAAGA
GATTTTCTTAGCCAATCCGAAAATTGCCTGTATTAGCTGTCATAATATTCCTGTGCTCTATGGTGAAACTGAAAAACTGG
ATGCATTTAAAAACTACGACTTAAGCTTAACGGAGAATTTCGATACCAATATCAATGGGGAAGAAGAAAACGATGTCGGT
GATTTGATCCCTGACTTGCCAGAACTTCAACCAACGGCACAAACACAATTAGATGAAAATAATCTTAGCTTTATGGCTGC
GGCTAAAACAGATAATGCTGAAACCCAATATGCACCGCGCGTGATTAGCGGTGAATTGGCCTTTTTAGTTCGCAGTGCTT
TAACCTCCGCTATTTATGGCGAGCCGGGTAAAAGCTGGGTGGGGACTAGCTGGCGCTTATCTAAAGCCTTTAAACGTCAA
GATATCGGTGGAAAAACCGGTACTACGAATAATTCGAAAGTTGCTTGGTACGCTGGCTTTGGCGCACATTTAACCACCGC
TGTGTATGTTGGCTTCGACGACAATAAGCGTAATTTAGGGCGCGGCGAAGCAGGGGCAAAAACGGCTTTACCTGCGTGGC
AAGCCTATATGCAAGTAAGCTTAGAAAATATTCCAGAACGTAAATTGAGTCCACCACCCAATATTATTGAAAAACGCATT
GATACGGGTTCTGGCTTACTCACGGAATCTGGTGGACGAATGGAATATTTTATCAATGGTACAGAACCTAAACGGAGTTA
TGTAGAAGAACAAGGCTACTACATCCCATCTGATGAGATAAACGAAAATTCCCCAATCCAAGAACGGGAAGAACTTTTCT
AA

Upstream 100 bases:

>100_bases
ACTGCACTTTTAGCTTTGGATTTAGTCTTGTTGTCTTTATACTGTGTACAATTTTTTCGAGTTTTGAATAAACAAACATT
AAACTAAAGAGAATTTCCCG

Downstream 100 bases:

>100_bases
AACAGACCGCACTTTTAAGACGCTATTTCTTTCAAATAGCGTCTTGTGCAAACCACGCATTCAATAAACAGCTGACGCCT
TCCACTAATTGTGTTTCACT

Product: PonA

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-binding protein A; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MRIAKLILSTLLTLIVLGCLALGAIYVYLKADLPDVESLKTVELQQPMQIYTADGKLIGEVGEQRRIPVPLDKIPKQLIH
AVLATEDNRFYEHHGLDPIGIARAIMVAVTKKGVSQGASTITQQLARNFFLTPEKSLERKAKEAVLAIDIENTLSKNEIL
ELYLNKIYLGYRSYGVAAAAKTYFGKELDQLSLSEIAIIAGLPKAPSTMNPIYSLKRAQERRNIVLGRMLTTKYITQEEY
DAAIKEPIVARYHGAQIDFRADYVTEMARQEMVRRFGEEKAYTSGFKVYTTVLSKDQAEAQKALRNNLIDYDMRHGYRGG
APLWQKDETVWDNDRIIGFLKKLPHSEPLLPAAVMNISKTGAELLLASGERMTLSHNAMRWAGKKTPKVGEQIWIRQKEK
GEWILAQVPEVNSALVSLNSDNGAIEALVGGFSFEQSKFNRATQSLVQVGSSIKPFIYAAALEKGLTLSSVLQDSPLVIQ
KAGQKPWQPKNSPNRFDGPMRLRVGLGLSKNMIAIRAMQMAGIDFTAEFLQRFGFKRDQYLASEALALGAASFTPLEMAR
GYAVFDNGGFLVDPFIINRILDNTGKEIFLANPKIACISCHNIPVLYGETEKLDAFKNYDLSLTENFDTNINGEEENDVG
DLIPDLPELQPTAQTQLDENNLSFMAAAKTDNAETQYAPRVISGELAFLVRSALTSAIYGEPGKSWVGTSWRLSKAFKRQ
DIGGKTGTTNNSKVAWYAGFGAHLTTAVYVGFDDNKRNLGRGEAGAKTALPAWQAYMQVSLENIPERKLSPPPNIIEKRI
DTGSGLLTESGGRMEYFINGTEPKRSYVEEQGYYIPSDEINENSPIQEREELF

Sequences:

>Translated_853_residues
MRIAKLILSTLLTLIVLGCLALGAIYVYLKADLPDVESLKTVELQQPMQIYTADGKLIGEVGEQRRIPVPLDKIPKQLIH
AVLATEDNRFYEHHGLDPIGIARAIMVAVTKKGVSQGASTITQQLARNFFLTPEKSLERKAKEAVLAIDIENTLSKNEIL
ELYLNKIYLGYRSYGVAAAAKTYFGKELDQLSLSEIAIIAGLPKAPSTMNPIYSLKRAQERRNIVLGRMLTTKYITQEEY
DAAIKEPIVARYHGAQIDFRADYVTEMARQEMVRRFGEEKAYTSGFKVYTTVLSKDQAEAQKALRNNLIDYDMRHGYRGG
APLWQKDETVWDNDRIIGFLKKLPHSEPLLPAAVMNISKTGAELLLASGERMTLSHNAMRWAGKKTPKVGEQIWIRQKEK
GEWILAQVPEVNSALVSLNSDNGAIEALVGGFSFEQSKFNRATQSLVQVGSSIKPFIYAAALEKGLTLSSVLQDSPLVIQ
KAGQKPWQPKNSPNRFDGPMRLRVGLGLSKNMIAIRAMQMAGIDFTAEFLQRFGFKRDQYLASEALALGAASFTPLEMAR
GYAVFDNGGFLVDPFIINRILDNTGKEIFLANPKIACISCHNIPVLYGETEKLDAFKNYDLSLTENFDTNINGEEENDVG
DLIPDLPELQPTAQTQLDENNLSFMAAAKTDNAETQYAPRVISGELAFLVRSALTSAIYGEPGKSWVGTSWRLSKAFKRQ
DIGGKTGTTNNSKVAWYAGFGAHLTTAVYVGFDDNKRNLGRGEAGAKTALPAWQAYMQVSLENIPERKLSPPPNIIEKRI
DTGSGLLTESGGRMEYFINGTEPKRSYVEEQGYYIPSDEINENSPIQEREELF
>Mature_853_residues
MRIAKLILSTLLTLIVLGCLALGAIYVYLKADLPDVESLKTVELQQPMQIYTADGKLIGEVGEQRRIPVPLDKIPKQLIH
AVLATEDNRFYEHHGLDPIGIARAIMVAVTKKGVSQGASTITQQLARNFFLTPEKSLERKAKEAVLAIDIENTLSKNEIL
ELYLNKIYLGYRSYGVAAAAKTYFGKELDQLSLSEIAIIAGLPKAPSTMNPIYSLKRAQERRNIVLGRMLTTKYITQEEY
DAAIKEPIVARYHGAQIDFRADYVTEMARQEMVRRFGEEKAYTSGFKVYTTVLSKDQAEAQKALRNNLIDYDMRHGYRGG
APLWQKDETVWDNDRIIGFLKKLPHSEPLLPAAVMNISKTGAELLLASGERMTLSHNAMRWAGKKTPKVGEQIWIRQKEK
GEWILAQVPEVNSALVSLNSDNGAIEALVGGFSFEQSKFNRATQSLVQVGSSIKPFIYAAALEKGLTLSSVLQDSPLVIQ
KAGQKPWQPKNSPNRFDGPMRLRVGLGLSKNMIAIRAMQMAGIDFTAEFLQRFGFKRDQYLASEALALGAASFTPLEMAR
GYAVFDNGGFLVDPFIINRILDNTGKEIFLANPKIACISCHNIPVLYGETEKLDAFKNYDLSLTENFDTNINGEEENDVG
DLIPDLPELQPTAQTQLDENNLSFMAAAKTDNAETQYAPRVISGELAFLVRSALTSAIYGEPGKSWVGTSWRLSKAFKRQ
DIGGKTGTTNNSKVAWYAGFGAHLTTAVYVGFDDNKRNLGRGEAGAKTALPAWQAYMQVSLENIPERKLSPPPNIIEKRI
DTGSGLLTESGGRMEYFINGTEPKRSYVEEQGYYIPSDEINENSPIQEREELF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=866, Percent_Identity=52.7713625866051, Blast_Score=882, Evalue=0.0,
Organism=Escherichia coli, GI1786343, Length=289, Percent_Identity=35.9861591695502, Blast_Score=156, Evalue=6e-39,
Organism=Escherichia coli, GI1788867, Length=184, Percent_Identity=37.5, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1789601, Length=138, Percent_Identity=40.5797101449275, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 94759; Mature: 94759

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAKLILSTLLTLIVLGCLALGAIYVYLKADLPDVESLKTVELQQPMQIYTADGKLIGE
CHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHCCCCEEEECCCHHHHH
VGEQRRIPVPLDKIPKQLIHAVLATEDNRFYEHHGLDPIGIARAIMVAVTKKGVSQGAST
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ITQQLARNFFLTPEKSLERKAKEAVLAIDIENTLSKNEILELYLNKIYLGYRSYGVAAAA
HHHHHHHHCCCCCHHHHHHHHHHEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTYFGKELDQLSLSEIAIIAGLPKAPSTMNPIYSLKRAQERRNIVLGRMLTTKYITQEEY
HHHHHHHHHHCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAIKEPIVARYHGAQIDFRADYVTEMARQEMVRRFGEEKAYTSGFKVYTTVLSKDQAEA
HHHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEHHHHCCCHHHH
QKALRNNLIDYDMRHGYRGGAPLWQKDETVWDNDRIIGFLKKLPHSEPLLPAAVMNISKT
HHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHC
GAELLLASGERMTLSHNAMRWAGKKTPKVGEQIWIRQKEKGEWILAQVPEVNSALVSLNS
CHHEEEECCCEEEEECCHHHHCCCCCCCCCHHHEEEECCCCCEEEEECCCCCCEEEEECC
DNGAIEALVGGFSFEQSKFNRATQSLVQVGSSIKPFIYAAALEKGLTLSSVLQDSPLVIQ
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEE
KAGQKPWQPKNSPNRFDGPMRLRVGLGLSKNMIAIRAMQMAGIDFTAEFLQRFGFKRDQY
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHEEHHHHCCCCHHHHHHHHHCCCHHHH
LASEALALGAASFTPLEMARGYAVFDNGGFLVDPFIINRILDNTGKEIFLANPKIACISC
HHHHHHHHCCCCCCHHHHHCCEEEEECCCEEECHHHHHHHHHCCCCEEEEECCCEEEEEE
HNIPVLYGETEKLDAFKNYDLSLTENFDTNINGEEENDVGDLIPDLPELQPTAQTQLDEN
CCCEEEECCCHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCCC
NLSFMAAAKTDNAETQYAPRVISGELAFLVRSALTSAIYGEPGKSWVGTSWRLSKAFKRQ
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
DIGGKTGTTNNSKVAWYAGFGAHLTTAVYVGFDDNKRNLGRGEAGAKTALPAWQAYMQVS
CCCCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
LENIPERKLSPPPNIIEKRIDTGSGLLTESGGRMEYFINGTEPKRSYVEEQGYYIPSDEI
HHHCCCCCCCCCHHHHHHHCCCCCCEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCCCC
NENSPIQEREELF
CCCCCCHHHHHCC
>Mature Secondary Structure
MRIAKLILSTLLTLIVLGCLALGAIYVYLKADLPDVESLKTVELQQPMQIYTADGKLIGE
CHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHCCCCEEEECCCHHHHH
VGEQRRIPVPLDKIPKQLIHAVLATEDNRFYEHHGLDPIGIARAIMVAVTKKGVSQGAST
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ITQQLARNFFLTPEKSLERKAKEAVLAIDIENTLSKNEILELYLNKIYLGYRSYGVAAAA
HHHHHHHHCCCCCHHHHHHHHHHEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTYFGKELDQLSLSEIAIIAGLPKAPSTMNPIYSLKRAQERRNIVLGRMLTTKYITQEEY
HHHHHHHHHHCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAIKEPIVARYHGAQIDFRADYVTEMARQEMVRRFGEEKAYTSGFKVYTTVLSKDQAEA
HHHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEHHHHCCCHHHH
QKALRNNLIDYDMRHGYRGGAPLWQKDETVWDNDRIIGFLKKLPHSEPLLPAAVMNISKT
HHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHC
GAELLLASGERMTLSHNAMRWAGKKTPKVGEQIWIRQKEKGEWILAQVPEVNSALVSLNS
CHHEEEECCCEEEEECCHHHHCCCCCCCCCHHHEEEECCCCCEEEEECCCCCCEEEEECC
DNGAIEALVGGFSFEQSKFNRATQSLVQVGSSIKPFIYAAALEKGLTLSSVLQDSPLVIQ
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEE
KAGQKPWQPKNSPNRFDGPMRLRVGLGLSKNMIAIRAMQMAGIDFTAEFLQRFGFKRDQY
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHEEHHHHCCCCHHHHHHHHHCCCHHHH
LASEALALGAASFTPLEMARGYAVFDNGGFLVDPFIINRILDNTGKEIFLANPKIACISC
HHHHHHHHCCCCCCHHHHHCCEEEEECCCEEECHHHHHHHHHCCCCEEEEECCCEEEEEE
HNIPVLYGETEKLDAFKNYDLSLTENFDTNINGEEENDVGDLIPDLPELQPTAQTQLDEN
CCCEEEECCCHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCCC
NLSFMAAAKTDNAETQYAPRVISGELAFLVRSALTSAIYGEPGKSWVGTSWRLSKAFKRQ
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
DIGGKTGTTNNSKVAWYAGFGAHLTTAVYVGFDDNKRNLGRGEAGAKTALPAWQAYMQVS
CCCCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
LENIPERKLSPPPNIIEKRIDTGSGLLTESGGRMEYFINGTEPKRSYVEEQGYYIPSDEI
HHHCCCCCCCCCHHHHHHHCCCCCCEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCCCC
NENSPIQEREELF
CCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1916268; 7542800; 7592463; 10675023 [H]