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The map label for this gene is aroK

Identifier: 15603089

GI number: 15603089

Start: 1415792

End: 1416319

Strand: Reverse

Name: aroK

Synonym: PM1224

Alternate gene names: 15603089

Gene position: 1416319-1415792 (Counterclockwise)

Preceding gene: 15603090

Following gene: 15603088

Centisome position: 62.74

GC content: 40.53

Gene sequence:

>528_bases
ATGGCAGAAAAGCGTAATATTTTCTTAATTGGACCAATGGGCGCGGGTAAAAGTACGATTGGCCGCCAATTAGCACAGCT
ATTAAGTATGGACTTTGTGGATTCGGATAATGAAATTGAACAACGAGCGGGTGCTGATATTAGTTGGATTTTTGATGTTG
AAGGTGAAGACGGTTTTCGTAAGCGTGAAGAACGCATTATTAATGAATTAACACAAAGACAAGGGATCGTGTTATCTACA
GGTGGTGGCGCGGTGTTATCGAAAGAAAATCGTAATCACTTATCAGCACGGGGTATCGTGATTTATTTAGAAACGACGGT
AGAGAAACAATATCAGCGTACACAGCGTGATAAAAAACGCCCTCTTTTGCAAGAGGTTGAGGATCCCCGTCAAGTCCTTG
AGGATTTAGCAAAAATTCGTAATCCTTTGTATGAAGAAATTGCCGATATCACCTTACCTACTGATGAACAAAGCGCTAAG
GTAATGGCAACGCAAATTGTCGATTTAATTGATAATCTTCACGGTTAG

Upstream 100 bases:

>100_bases
TATTTTTTGGGTGATCTTTCTGCGGCCGATAAGGGTCGCTTCTCTATTTTGTAAAAGATCATAATTATCAATGTATTAAT
GAAAAGAAGAAAAATTAGAG

Downstream 100 bases:

>100_bases
ATAAGTAGAGTAAAGCATGTTGTGTGTAAATGTTGAGTTAAAAGAGCGCCGTTATCCAATTTATATTGGTTCGGGATTAC
TTTCGGATGAAAAATGCTAT

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MAEKRNIFLIGPMGAGKSTIGRQLAQLLSMDFVDSDNEIEQRAGADISWIFDVEGEDGFRKREERIINELTQRQGIVLST
GGGAVLSKENRNHLSARGIVIYLETTVEKQYQRTQRDKKRPLLQEVEDPRQVLEDLAKIRNPLYEEIADITLPTDEQSAK
VMATQIVDLIDNLHG

Sequences:

>Translated_175_residues
MAEKRNIFLIGPMGAGKSTIGRQLAQLLSMDFVDSDNEIEQRAGADISWIFDVEGEDGFRKREERIINELTQRQGIVLST
GGGAVLSKENRNHLSARGIVIYLETTVEKQYQRTQRDKKRPLLQEVEDPRQVLEDLAKIRNPLYEEIADITLPTDEQSAK
VMATQIVDLIDNLHG
>Mature_174_residues
AEKRNIFLIGPMGAGKSTIGRQLAQLLSMDFVDSDNEIEQRAGADISWIFDVEGEDGFRKREERIINELTQRQGIVLSTG
GGAVLSKENRNHLSARGIVIYLETTVEKQYQRTQRDKKRPLLQEVEDPRQVLEDLAKIRNPLYEEIADITLPTDEQSAKV
MATQIVDLIDNLHG

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=172, Percent_Identity=70.9302325581395, Blast_Score=261, Evalue=2e-71,
Organism=Escherichia coli, GI1786587, Length=166, Percent_Identity=33.1325301204819, Blast_Score=86, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320332, Length=137, Percent_Identity=37.2262773722628, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_PASMU (P57925)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246161.1
- ProteinModelPortal:   P57925
- SMR:   P57925
- GeneID:   1244571
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1224
- NMPDR:   fig|272843.1.peg.1224
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK00131
- BioCyc:   PMUL272843:PM1224-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19791; Mature: 19660

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 140-140 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKRNIFLIGPMGAGKSTIGRQLAQLLSMDFVDSDNEIEQRAGADISWIFDVEGEDGFR
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCHH
KREERIINELTQRQGIVLSTGGGAVLSKENRNHLSARGIVIYLETTVEKQYQRTQRDKKR
HHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCC
PLLQEVEDPRQVLEDLAKIRNPLYEEIADITLPTDEQSAKVMATQIVDLIDNLHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKRNIFLIGPMGAGKSTIGRQLAQLLSMDFVDSDNEIEQRAGADISWIFDVEGEDGFR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCHH
KREERIINELTQRQGIVLSTGGGAVLSKENRNHLSARGIVIYLETTVEKQYQRTQRDKKR
HHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCC
PLLQEVEDPRQVLEDLAKIRNPLYEEIADITLPTDEQSAKVMATQIVDLIDNLHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100