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The map label for this gene is recF

Identifier: 15603024

GI number: 15603024

Start: 1354795

End: 1355871

Strand: Reverse

Name: recF

Synonym: PM1159

Alternate gene names: 15603024

Gene position: 1355871-1354795 (Counterclockwise)

Preceding gene: 15603025

Following gene: 15603017

Centisome position: 60.06

GC content: 40.95

Gene sequence:

>1077_bases
ATGGCAATTTCACGTTTACTGATTGAAAACTTTCGTAACTTAACAGCCGTGGATCTCGAATTTGATCACGGCTTTAACTT
TTTAGTGGGTAACAATGGCAGTGGCAAAACCAGCCTATTAGAAGCGATTTTTTATCTCGGGCATGGTCGTTCCTTCAAAA
GTGCGGTCAGTAATCGCATTATTTCTTACGAACAACCGCATTTTATTTTACACGCCAAAATTCAAGAACAAGCCCATCAA
TGGTCTGTTGGTTTACAGAAATTACGGCAAGGAAATTCAGTTGTAAAAATTAATGGTGAAGATGGCAATAAAATTGCCGA
TCTTGCCCATTTGCTTCCTATGCAATTAATTACCCCTGAAGGCTTAACCTTGTTAAATGGTGGTCCCGTTTACCGTCGTG
CTTTTTTGGACTGGGGACTTTTTCATCATCATAATCACTTTCATTTAGCTTGGGTAAATTTAAATCGTTTATTAAAACAA
CGTAATGCGGCGTTACAACAAGCCACTCATTATCAGGCGCTTGAAATCTGGGATCGTGAACTAGTGAAACTTGCGCATCA
AGTCAGTGAATGGCGTGCACAATATGCAGAAGCATTGCGTCCTGAAATTGAACAGACTTGCCGCTTATTTTTACCTGAAT
TAGAGATCAGTGTGAGCTTTCATCAAGGTTGGGAAAAAGACAGTGACTATGCTGACTTGTTAGTTCGCCATTTTGAACGC
GATCGTGCAATAGGTTATACCGTGTCGGGACCACAAAAAGCAGACTTTCGCTTTAAAGCCAATGGCTTGCCAGTGGAAGA
TGTGCTTTCTCGTGGGCAGTTGAAGTTATTGATGTGTGCACTACGCCTTGCGCAAGGTGAACATTTAATGAAGCAGAAAG
AACGACATTGTATTTTTCTGATTGATGATTTTGCTTCGGAACTGGATCAATATAAACGCGCTTTATTAGCTGAACGTTTG
AAACAAAGTGGCTCACAAGTGTTTGTCAGCGCGATTACAGAAACACAACTAAAGCAAATGCAACCACAACAACACCGCAC
TTTTCGGGTTGAAGAAGGTTGCATTGCACCGCTTTAA

Upstream 100 bases:

>100_bases
TGACCGATGCCTCGTCAAGTTGCTTAATTGAAGATTGTGACGATGCTAGCGCGGAATATGTCATTATGCCAATGCGTTTA
TAGTTTTCTTTGCACACTGT

Downstream 100 bases:

>100_bases
ATGGCTTATTTTTGACTACAAACCGCAATAAAATTGAGCTTATTTTGTGGGTCATTAAAGGTTTTGAACATTTGTTTAAA
TTGTTCTCGATTTTCTGACT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MAISRLLIENFRNLTAVDLEFDHGFNFLVGNNGSGKTSLLEAIFYLGHGRSFKSAVSNRIISYEQPHFILHAKIQEQAHQ
WSVGLQKLRQGNSVVKINGEDGNKIADLAHLLPMQLITPEGLTLLNGGPVYRRAFLDWGLFHHHNHFHLAWVNLNRLLKQ
RNAALQQATHYQALEIWDRELVKLAHQVSEWRAQYAEALRPEIEQTCRLFLPELEISVSFHQGWEKDSDYADLLVRHFER
DRAIGYTVSGPQKADFRFKANGLPVEDVLSRGQLKLLMCALRLAQGEHLMKQKERHCIFLIDDFASELDQYKRALLAERL
KQSGSQVFVSAITETQLKQMQPQQHRTFRVEEGCIAPL

Sequences:

>Translated_358_residues
MAISRLLIENFRNLTAVDLEFDHGFNFLVGNNGSGKTSLLEAIFYLGHGRSFKSAVSNRIISYEQPHFILHAKIQEQAHQ
WSVGLQKLRQGNSVVKINGEDGNKIADLAHLLPMQLITPEGLTLLNGGPVYRRAFLDWGLFHHHNHFHLAWVNLNRLLKQ
RNAALQQATHYQALEIWDRELVKLAHQVSEWRAQYAEALRPEIEQTCRLFLPELEISVSFHQGWEKDSDYADLLVRHFER
DRAIGYTVSGPQKADFRFKANGLPVEDVLSRGQLKLLMCALRLAQGEHLMKQKERHCIFLIDDFASELDQYKRALLAERL
KQSGSQVFVSAITETQLKQMQPQQHRTFRVEEGCIAPL
>Mature_357_residues
AISRLLIENFRNLTAVDLEFDHGFNFLVGNNGSGKTSLLEAIFYLGHGRSFKSAVSNRIISYEQPHFILHAKIQEQAHQW
SVGLQKLRQGNSVVKINGEDGNKIADLAHLLPMQLITPEGLTLLNGGPVYRRAFLDWGLFHHHNHFHLAWVNLNRLLKQR
NAALQQATHYQALEIWDRELVKLAHQVSEWRAQYAEALRPEIEQTCRLFLPELEISVSFHQGWEKDSDYADLLVRHFERD
RAIGYTVSGPQKADFRFKANGLPVEDVLSRGQLKLLMCALRLAQGEHLMKQKERHCIFLIDDFASELDQYKRALLAERLK
QSGSQVFVSAITETQLKQMQPQQHRTFRVEEGCIAPL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=57.8651685393258, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_PASMU (Q9CLQ6)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246096.1
- ProteinModelPortal:   Q9CLQ6
- GeneID:   1244506
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1159
- NMPDR:   fig|272843.1.peg.1159
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   PMUL272843:PM1159-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41147; Mature: 41015

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISRLLIENFRNLTAVDLEFDHGFNFLVGNNGSGKTSLLEAIFYLGHGRSFKSAVSNRI
CCHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCC
ISYEQPHFILHAKIQEQAHQWSVGLQKLRQGNSVVKINGEDGNKIADLAHLLPMQLITPE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
GLTLLNGGPVYRRAFLDWGLFHHHNHFHLAWVNLNRLLKQRNAALQQATHYQALEIWDRE
CCEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVKLAHQVSEWRAQYAEALRPEIEQTCRLFLPELEISVSFHQGWEKDSDYADLLVRHFER
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEHHCCCCCCCCHHHHHHHHHHH
DRAIGYTVSGPQKADFRFKANGLPVEDVLSRGQLKLLMCALRLAQGEHLMKQKERHCIFL
CCCCEEEECCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEE
IDDFASELDQYKRALLAERLKQSGSQVFVSAITETQLKQMQPQQHRTFRVEEGCIAPL
EHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCHHHCCEEECCCCCCCC
>Mature Secondary Structure 
AISRLLIENFRNLTAVDLEFDHGFNFLVGNNGSGKTSLLEAIFYLGHGRSFKSAVSNRI
CHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCC
ISYEQPHFILHAKIQEQAHQWSVGLQKLRQGNSVVKINGEDGNKIADLAHLLPMQLITPE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
GLTLLNGGPVYRRAFLDWGLFHHHNHFHLAWVNLNRLLKQRNAALQQATHYQALEIWDRE
CCEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVKLAHQVSEWRAQYAEALRPEIEQTCRLFLPELEISVSFHQGWEKDSDYADLLVRHFER
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEHHCCCCCCCCHHHHHHHHHHH
DRAIGYTVSGPQKADFRFKANGLPVEDVLSRGQLKLLMCALRLAQGEHLMKQKERHCIFL
CCCCEEEECCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEE
IDDFASELDQYKRALLAERLKQSGSQVFVSAITETQLKQMQPQQHRTFRVEEGCIAPL
EHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCHHHCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100