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The map label for this gene is dnaN [H]

Identifier: 15603025

GI number: 15603025

Start: 1355889

End: 1356989

Strand: Reverse

Name: dnaN [H]

Synonym: PM1160

Alternate gene names: 15603025

Gene position: 1356989-1355889 (Counterclockwise)

Preceding gene: 15603026

Following gene: 15603024

Centisome position: 60.11

GC content: 40.33

Gene sequence:

>1101_bases
ATGCAGTTTATTGTTTCAAGAGAAAATTTATTAAAGCCATTACAACAAGTGTGTGGGGTGCTAAGTAGCCGCCCAAATAT
TCCCGTATTAAATAATGTATTACTACAAATTCGTGGTGAGCGTTTAGTGATTACCGGCACGGACTTAGAAGTTGAGCTCT
CCACTGAAACTGCACTATTACGTACAGATGTGCAAGGTAGTTTTACTATTCCTGCCAAGAAATTTTTAGATATTTGCCGC
AGTTTACCCGAAGAAGCGGAGATCTCAGTCAGTTTCGAAGAAGATCGTGCCATTGTGAAATCTGGTCGCAGTAAATTTAA
CTTATCGACTTTACCAGCTGAAGAATACCCAAATTTAACTGATTGGCAATCAGAAGTTGATTTTACTCTTGAGCAAAGCA
CATTGCGTCGTCTGATTGAAGCCACGCAATTTTCCATGGCGAACCAAGACGCACGCTATTTCTTAAACGGCATGAAATTT
GAAACAGAAGGGAATTTATTGCGTACTGTGGCAACGGATGGTCACCGTTTAGCCGTTTGTACCATTGCATTAGAACAAGA
TTTACAAACCCATTCAGTGATATTACCGCGCAAAGGGGTATTAGAGTTAGTCCGTTTACTCGAAAACACGGATTTGCCCG
CCCGTTTACAAATTGGTACGAATAACTTGCGTATTGATTTAGGCAATATTATTTTCACTTCAAAACTGATCGATGGTCGA
TTCCCAGATTATCGTCGTGTCTTACCACGCAATGCAACACGTATTTTAGAAGCGGAATGGGATGTGTTAAAACAGGCGTT
TGTGCGCGCGGCGATTTTATCTAATGAGCGTTTCCGTAGTGTTCGTTTACAGTTAAGTGAAAATCAATTAAAAATTACGG
CAACCAATCCAGAACAAGAAGTGGCAGAAGAAATTATCGATGTTTCTTATGTGGGTGAAGAAATGGAAGTCGGCTTTAAT
GTAAGCTACATTTTAGATGTATTAAATGCGTTAAAATGTCAGCGAGTGCGTATGCGTCTGACCGATGCCTCGTCAAGTTG
CTTAATTGAAGATTGTGACGATGCTAGCGCGGAATATGTCATTATGCCAATGCGTTTATAG

Upstream 100 bases:

>100_bases
GCCTGTCGTAAAATTGCAGAATTGAGAGAACAAGAACATTCTTTAGAAGAGGATTGGTCAAATTTAATCCGTACACTCTC
TGTGTAAAAAGGGGAAAAAT

Downstream 100 bases:

>100_bases
TTTTCTTTGCACACTGTATGGCAATTTCACGTTTACTGATTGAAAACTTTCGTAACTTAACAGCCGTGGATCTCGAATTT
GATCACGGCTTTAACTTTTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQFIVSRENLLKPLQQVCGVLSSRPNIPVLNNVLLQIRGERLVITGTDLEVELSTETALLRTDVQGSFTIPAKKFLDICR
SLPEEAEISVSFEEDRAIVKSGRSKFNLSTLPAEEYPNLTDWQSEVDFTLEQSTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTIALEQDLQTHSVILPRKGVLELVRLLENTDLPARLQIGTNNLRIDLGNIIFTSKLIDGR
FPDYRRVLPRNATRILEAEWDVLKQAFVRAAILSNERFRSVRLQLSENQLKITATNPEQEVAEEIIDVSYVGEEMEVGFN
VSYILDVLNALKCQRVRMRLTDASSSCLIEDCDDASAEYVIMPMRL

Sequences:

>Translated_366_residues
MQFIVSRENLLKPLQQVCGVLSSRPNIPVLNNVLLQIRGERLVITGTDLEVELSTETALLRTDVQGSFTIPAKKFLDICR
SLPEEAEISVSFEEDRAIVKSGRSKFNLSTLPAEEYPNLTDWQSEVDFTLEQSTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTIALEQDLQTHSVILPRKGVLELVRLLENTDLPARLQIGTNNLRIDLGNIIFTSKLIDGR
FPDYRRVLPRNATRILEAEWDVLKQAFVRAAILSNERFRSVRLQLSENQLKITATNPEQEVAEEIIDVSYVGEEMEVGFN
VSYILDVLNALKCQRVRMRLTDASSSCLIEDCDDASAEYVIMPMRL
>Mature_366_residues
MQFIVSRENLLKPLQQVCGVLSSRPNIPVLNNVLLQIRGERLVITGTDLEVELSTETALLRTDVQGSFTIPAKKFLDICR
SLPEEAEISVSFEEDRAIVKSGRSKFNLSTLPAEEYPNLTDWQSEVDFTLEQSTLRRLIEATQFSMANQDARYFLNGMKF
ETEGNLLRTVATDGHRLAVCTIALEQDLQTHSVILPRKGVLELVRLLENTDLPARLQIGTNNLRIDLGNIIFTSKLIDGR
FPDYRRVLPRNATRILEAEWDVLKQAFVRAAILSNERFRSVRLQLSENQLKITATNPEQEVAEEIIDVSYVGEEMEVGFN
VSYILDVLNALKCQRVRMRLTDASSSCLIEDCDDASAEYVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=66.120218579235, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41481; Mature: 41481

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFIVSRENLLKPLQQVCGVLSSRPNIPVLNNVLLQIRGERLVITGTDLEVELSTETALL
CCEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCEEEEEECCEEEEECCCEEEE
RTDVQGSFTIPAKKFLDICRSLPEEAEISVSFEEDRAIVKSGRSKFNLSTLPAEEYPNLT
EECCCCCEECCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCEECCCHHHCCCCC
DWQSEVDFTLEQSTLRRLIEATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCHHCCEEECHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TIALEQDLQTHSVILPRKGVLELVRLLENTDLPARLQIGTNNLRIDLGNIIFTSKLIDGR
EEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCEEEEEHHHCCC
FPDYRRVLPRNATRILEAEWDVLKQAFVRAAILSNERFRSVRLQLSENQLKITATNPEQE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCEEEEEECCCHHH
VAEEIIDVSYVGEEMEVGFNVSYILDVLNALKCQRVRMRLTDASSSCLIEDCDDASAEYV
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCCCCCCEE
IMPMRL
EEEECC
>Mature Secondary Structure
MQFIVSRENLLKPLQQVCGVLSSRPNIPVLNNVLLQIRGERLVITGTDLEVELSTETALL
CCEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCEEEEEECCEEEEECCCEEEE
RTDVQGSFTIPAKKFLDICRSLPEEAEISVSFEEDRAIVKSGRSKFNLSTLPAEEYPNLT
EECCCCCEECCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCCEECCCHHHCCCCC
DWQSEVDFTLEQSTLRRLIEATQFSMANQDARYFLNGMKFETEGNLLRTVATDGHRLAVC
CCCHHCCEEECHHHHHHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCCCEEEEE
TIALEQDLQTHSVILPRKGVLELVRLLENTDLPARLQIGTNNLRIDLGNIIFTSKLIDGR
EEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCEEEEEHHHCCC
FPDYRRVLPRNATRILEAEWDVLKQAFVRAAILSNERFRSVRLQLSENQLKITATNPEQE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCEEEEEECCCHHH
VAEEIIDVSYVGEEMEVGFNVSYILDVLNALKCQRVRMRLTDASSSCLIEDCDDASAEYV
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCCCCCCEE
IMPMRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]