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The map label for this gene is gltX

Identifier: 15602980

GI number: 15602980

Start: 1312375

End: 1313817

Strand: Reverse

Name: gltX

Synonym: PM1115

Alternate gene names: 15602980

Gene position: 1313817-1312375 (Counterclockwise)

Preceding gene: 15602982

Following gene: 15602979

Centisome position: 58.2

GC content: 42.76

Gene sequence:

>1443_bases
ATGAAAGCAGAAGCATTATTTGATTTAGATCCTGGTGTGAAAGTGCGCACTCGTTTTGCGCCAAGTCCAACAGGCTATTT
ACATGTAGGGGGCGCTAGAACGGCACTTTATTCTTGGCTCTATGCAAAACATCACCAAGGCGAATTTGTTTTACGTATTG
AAGATACCGACTTAGAGCGCTCAACACCAGAAGCGACTGCTGCCATTTTAGAAGGAATGGCGTGGTTAAATTTAGCTTGG
GAACATGGTCCATATTTTCAAACCAAGCGTTTTGATCGTTATAACCAAGTGATTGATCAAATGATCGAACAAGGGTTAGC
CTACCGTTGTTATTGTTCAAAAGAACGTTTAGAAGATTTACGCAACACGCAAGAACAGAATAAACAAAAACCCCGCTATG
ATCGTCATTGTTTAGGTGATCATAAGCACTCGCCAGAACAACCGCATGTGGTGCGTTTCAAAAATCCAACGGAAGGCTCC
GTGGTGTTTGAAGATGCAGTGCGTGGACGTATTGAGATCAGTAACGCAGAGTTAGATGATTTGATTATTCGCCGTACCGA
TGGCTCACCGACTTATAATTTCTGTGTGGTGGTGGATGACTGGGATATGGGCATCACGCATGTGGTGCGCGGTGAAGACC
ATATCAACAATACGCCGCGTCAAATTAATATTTTAAAAGCTTTAGGCGCACCCATTCCAACTTACGCCCATGTGTCGATG
ATTTTAGGTGATGATGGACAAAAATTATCCAAACGTCATGGCGCGGTGAGCGTGATGCAATATCGTGATGATGGTTACTT
GCCTGAAGCGTTATTAAATTATTTGGTGCGTTTAGGTTGGGGACATGGCGATCAAGAGATTTTCTCCGTAGAAGAAATGA
TCAAGTTATTTGAGCTTGAAAGTGTGAGTAAATCAGCCAGTGCGTTTAATACGGAAAAATTATTGTGGTTAAATCATCAC
TATATACGTGAATTGCCAGCAGAATATGTGGCTAAACATTTAGCATGGCACTATCAGGATCAAGGCATTGATACCTCCAA
TGGACCTGCATTAGAAGACATCGTAAAAATGTTGGCTGAACGTTGTAAGACACTCAAAGAAATGGCTGCCGCAAGCCGTT
ATTTCTTCGAAGATTTCGACAGCTTTGATGAAGCCGCAGTGAAAAAACATTTCAAAGTCGCCGCGATTGAGCCACTTGAG
AAAGTTAAAGAAAAATTGACCGCACTTGATAGCTGGGATTTACACAGCACGCATCAAGCCATTGAACAGACTGCCGCTGA
ATTAGAATTGGGGATGGGCAAGGTTGGGATGCCATTGCGTGTTGCGGTAACTGGTTCTGGTCAATCACCTTCTATGGATG
TAACTCTAGTGGGCATTGGCAAGGCACGTACGCTTGTGCGTATTCAAAAAGCGATTGATTTTATTAAAAGTCAAAATGTC
TAA

Upstream 100 bases:

>100_bases
AGGCATAAAATCACATTTTTTATTCGGAAATATGGAAACTCATAGTACAATATGGCACAAATTTTTTGATGTAATTTAAG
GTAAGAGAAAAGGTTATAAG

Downstream 100 bases:

>100_bases
TATTTAGGCTTCTGTTTAAATATCAGCAATTTGAGGTTGACAGTTTGTGTGGCGGAATTTATTATTACCGCCACGACATT
TCTGATGTGGGGATATAGCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKAEALFDLDPGVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHHQGEFVLRIEDTDLERSTPEATAAILEGMAWLNLAW
EHGPYFQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLEDLRNTQEQNKQKPRYDRHCLGDHKHSPEQPHVVRFKNPTEGS
VVFEDAVRGRIEISNAELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
ILGDDGQKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSVEEMIKLFELESVSKSASAFNTEKLLWLNHH
YIRELPAEYVAKHLAWHYQDQGIDTSNGPALEDIVKMLAERCKTLKEMAAASRYFFEDFDSFDEAAVKKHFKVAAIEPLE
KVKEKLTALDSWDLHSTHQAIEQTAAELELGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGKARTLVRIQKAIDFIKSQNV

Sequences:

>Translated_480_residues
MKAEALFDLDPGVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHHQGEFVLRIEDTDLERSTPEATAAILEGMAWLNLAW
EHGPYFQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLEDLRNTQEQNKQKPRYDRHCLGDHKHSPEQPHVVRFKNPTEGS
VVFEDAVRGRIEISNAELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
ILGDDGQKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSVEEMIKLFELESVSKSASAFNTEKLLWLNHH
YIRELPAEYVAKHLAWHYQDQGIDTSNGPALEDIVKMLAERCKTLKEMAAASRYFFEDFDSFDEAAVKKHFKVAAIEPLE
KVKEKLTALDSWDLHSTHQAIEQTAAELELGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGKARTLVRIQKAIDFIKSQNV
>Mature_480_residues
MKAEALFDLDPGVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHHQGEFVLRIEDTDLERSTPEATAAILEGMAWLNLAW
EHGPYFQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLEDLRNTQEQNKQKPRYDRHCLGDHKHSPEQPHVVRFKNPTEGS
VVFEDAVRGRIEISNAELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
ILGDDGQKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSVEEMIKLFELESVSKSASAFNTEKLLWLNHH
YIRELPAEYVAKHLAWHYQDQGIDTSNGPALEDIVKMLAERCKTLKEMAAASRYFFEDFDSFDEAAVKKHFKVAAIEPLE
KVKEKLTALDSWDLHSTHQAIEQTAAELELGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGKARTLVRIQKAIDFIKSQNV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=509, Percent_Identity=31.8271119842829, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI62241042, Length=398, Percent_Identity=23.6180904522613, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4826960, Length=302, Percent_Identity=25.8278145695364, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=73.0603448275862, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=300, Percent_Identity=35.3333333333333, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1786895, Length=342, Percent_Identity=21.9298245614035, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=31.340206185567, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17511007, Length=255, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=337, Percent_Identity=38.5756676557863, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324742, Length=212, Percent_Identity=28.7735849056604, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321192, Length=243, Percent_Identity=29.2181069958848, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=31.3765182186235, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI17864488, Length=171, Percent_Identity=30.9941520467836, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PASMU (P57906)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246052.1
- ProteinModelPortal:   P57906
- SMR:   P57906
- GeneID:   1244462
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1115
- NMPDR:   fig|272843.1.peg.1115
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- ProtClustDB:   PRK01406
- BioCyc:   PMUL272843:PM1115-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54480; Mature: 54480

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAEALFDLDPGVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHHQGEFVLRIEDTDLER
CCCCEEEECCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCC
STPEATAAILEGMAWLNLAWEHGPYFQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLEDL
CCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
RNTQEQNKQKPRYDRHCLGDHKHSPEQPHVVRFKNPTEGSVVFEDAVRGRIEISNAELDD
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCC
LIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCHHEEEE
ILGDDGQKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSVEEMIKLFELE
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SVSKSASAFNTEKLLWLNHHYIRELPAEYVAKHLAWHYQDQGIDTSNGPALEDIVKMLAE
HHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RCKTLKEMAAASRYFFEDFDSFDEAAVKKHFKVAAIEPLEKVKEKLTALDSWDLHSTHQA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IEQTAAELELGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGKARTLVRIQKAIDFIKSQNV
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKAEALFDLDPGVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHHQGEFVLRIEDTDLER
CCCCEEEECCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCC
STPEATAAILEGMAWLNLAWEHGPYFQTKRFDRYNQVIDQMIEQGLAYRCYCSKERLEDL
CCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
RNTQEQNKQKPRYDRHCLGDHKHSPEQPHVVRFKNPTEGSVVFEDAVRGRIEISNAELDD
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCC
LIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKALGAPIPTYAHVSM
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCHHCCCCCHHHHHHHHHCCCCCCHHEEEE
ILGDDGQKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWGHGDQEIFSVEEMIKLFELE
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SVSKSASAFNTEKLLWLNHHYIRELPAEYVAKHLAWHYQDQGIDTSNGPALEDIVKMLAE
HHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RCKTLKEMAAASRYFFEDFDSFDEAAVKKHFKVAAIEPLEKVKEKLTALDSWDLHSTHQA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IEQTAAELELGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGKARTLVRIQKAIDFIKSQNV
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100