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The map label for this gene is pnp

Identifier: 15602979

GI number: 15602979

Start: 1309782

End: 1311926

Strand: Reverse

Name: pnp

Synonym: PM1114

Alternate gene names: 15602979

Gene position: 1311926-1309782 (Counterclockwise)

Preceding gene: 15602980

Following gene: 161723311

Centisome position: 58.11

GC content: 43.64

Gene sequence:

>2145_bases
GTGAATCCAATTGTTAAACAATTTAAATATGGTCAACATACTGTTACGTTAGAAACGGGTGCAATTGCACGCCAAGCAAC
GGCAGCAGTGATGGCAAGTATGGATGATACTACTGTGTTTGTCACAGTGGTTGCTAAAAAAGATGTGAAAGAAGGTCAAG
ACTTCTTCCCATTAACCGTTAACTATCAAGAGCGTACTTATGCTGCGGGTCGTATTCCTGGTGGCTTTTTCAAACGTGAA
GGTCGTCCTTCTGAAGGCGAAACCTTAATTGCACGCTTAATTGACCGTCCAATTCGTCCTCTTTTCCCAGAAGGTTTTTA
TAACGAAATCCAAATCGTGGCGACAGTGGTGTCTGTTAATCCGCAAATTTGCCCAGATTTAGTGGCAATGATCGGTGCAT
CTGCGGCACTTTCTTTATCAGGTGTGCCATTTAATGGCCCTATCGGTGCAGCACGTGTTGGTTTTATTGATGATCAATTT
GTGTTAAACCCAACCATGAACGAGCAAAAACAAAGCCGTTTAGACTTGGTTGTCGCGGGAACAGATAAAGCAGTGTTAAT
GGTGGAATCTGAAGCCGATGTATTAACCGAAGAACAAATGTTAGCTGCGGTGGTGTTTGGTCATCAGCAACAACAAGTGG
TGATTGACGCGATCAAAGAATTTACCGCAGAAGCCGGTAAACCGCGTTGGGATTGGGTGGCACCTGAACCAAATACCGCG
TTAATTGAAAAAGTGAAAGCGATTGCAGAAGCGCGTCTAGGCGAAGCATACCGTATCACTGAAAAACAAGCACGTTATGA
ACAAATTGATGCGATTAAAGCTGATGTGATTGCACAAATCACAGCTGAAGTGGCAGAAGGCGAAGACATCAGTGAAGGGA
AAATTGTCGATATTTTCACCGCACTTGAAAGCCAAATCGTACGTAGCCGTATTATTGCTGGTGAACCACGTATTGATGGT
CGTACAGTAGATACTGTTCGTGCATTAGATATTTGTACTGGTGTTTTACCACGTACACACGGTTCTGCGATTTTCACCCG
TGGTGAAACACAGGCGTTAGCTGTTGCGACATTAGGTACAGAACGTGATGCACAAATTATTGATGAATTAACAGGTGAGC
GTTCAGATCATTTCTTATTCCACTACAACTTCCCGCCATATTCTGTGGGTGAAACCGGTATGATTGGTTCACCAAAACGT
CGTGAAATTGGTCATGGTCGTTTAGCGAAACGCGGTGTGGCTGCAGTGATGCCAACACTTGCCGAGTTCCCGTATGTGGT
ACGTGTTGTCTCTGAAATCACAGAATCAAATGGTTCTTCTTCTATGGCATCGGTTTGTGGTGCGTCTTTAGCATTAATGG
ATGCGGGTGTACCAATTAAAGCGGCGGTTGCAGGTATTGCAATGGGCTTAGTCAAAGAAGACGAAAAATTTGTGGTGCTT
TCAGACATCTTAGGTGATGAAGATCACTTAGGTGACATGGATTTTAAAGTGGCGGGTACACGTACTGGTGTAACGGCATT
ACAAATGGATATCAAAATCGAAGGTATCACAGCAGAAATCATGCAAATTGCGTTAAACCAAGCGAAAAGTGCACGTTTAC
ACATTTTAGGTGTGATGGAGCAAGCGATTCCTGCGCCACGTGCGGATATTTCTGATTTTGCACCGCGTATTTACACCATG
AAAATTGATCCGAAGAAAATCAAAGATGTGATCGGTAAAGGTGGTGCAACCATTCGTGCCTTAACAGAAGAAACAGGTAC
CTCAATTGATATCGATGATGATGGTACAGTGAAGATTGCGGCGGTTGATGGCAATTCAGCAAAAGAGGTGATGGCGCGTA
TTGAAGATATTACTGCAGAAGTTGAAGCGGGTGCAGTGTATAAAGGCAAAGTTACTCGTTTAGCTGATTTTGGTGCCTTC
GTTTCTATCGTAGGTAACAAAGAAGGCTTAGTGCATATTTCTCAAATCGCGGAAGAGCGTGTTGAGAAAGTGAGTGATTA
TCTTGCGGTGGGACAAGAAGTGACTGTTAAAGTGGTTGAGATTGATCGTCAAGGTCGTATTCGTTTAACCATGAAAGAAG
TTGCACCAAAGCAAGAACACGTTGATTCTGTTGTCGCAGACGTTGCCGCAGAAGAAAACGCATAA

Upstream 100 bases:

>100_bases
GATTATTTTTGCTAGTCGCAATTGGAAAATAGAGCCATTTTTTTATTCACAAGTTAATGAAGTTGTTGATTTCATTAGCT
ATTTTAAAAGGAACATTCTC

Downstream 100 bases:

>100_bases
GCAATAAACACCAACGCCCTTCGTGATAAAGGGCCTTGGTGTGCATGTTGATAAGTACAATTTGTGCTTTAAGGCGAAGC
GAAATGAAGCAATTTCATCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTVNYQERTYAAGRIPGGFFKRE
GRPSEGETLIARLIDRPIRPLFPEGFYNEIQIVATVVSVNPQICPDLVAMIGASAALSLSGVPFNGPIGAARVGFIDDQF
VLNPTMNEQKQSRLDLVVAGTDKAVLMVESEADVLTEEQMLAAVVFGHQQQQVVIDAIKEFTAEAGKPRWDWVAPEPNTA
LIEKVKAIAEARLGEAYRITEKQARYEQIDAIKADVIAQITAEVAEGEDISEGKIVDIFTALESQIVRSRIIAGEPRIDG
RTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGERSDHFLFHYNFPPYSVGETGMIGSPKR
REIGHGRLAKRGVAAVMPTLAEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEDEKFVVL
SDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIEGITAEIMQIALNQAKSARLHILGVMEQAIPAPRADISDFAPRIYTM
KIDPKKIKDVIGKGGATIRALTEETGTSIDIDDDGTVKIAAVDGNSAKEVMARIEDITAEVEAGAVYKGKVTRLADFGAF
VSIVGNKEGLVHISQIAEERVEKVSDYLAVGQEVTVKVVEIDRQGRIRLTMKEVAPKQEHVDSVVADVAAEENA

Sequences:

>Translated_714_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTVNYQERTYAAGRIPGGFFKRE
GRPSEGETLIARLIDRPIRPLFPEGFYNEIQIVATVVSVNPQICPDLVAMIGASAALSLSGVPFNGPIGAARVGFIDDQF
VLNPTMNEQKQSRLDLVVAGTDKAVLMVESEADVLTEEQMLAAVVFGHQQQQVVIDAIKEFTAEAGKPRWDWVAPEPNTA
LIEKVKAIAEARLGEAYRITEKQARYEQIDAIKADVIAQITAEVAEGEDISEGKIVDIFTALESQIVRSRIIAGEPRIDG
RTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGERSDHFLFHYNFPPYSVGETGMIGSPKR
REIGHGRLAKRGVAAVMPTLAEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEDEKFVVL
SDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIEGITAEIMQIALNQAKSARLHILGVMEQAIPAPRADISDFAPRIYTM
KIDPKKIKDVIGKGGATIRALTEETGTSIDIDDDGTVKIAAVDGNSAKEVMARIEDITAEVEAGAVYKGKVTRLADFGAF
VSIVGNKEGLVHISQIAEERVEKVSDYLAVGQEVTVKVVEIDRQGRIRLTMKEVAPKQEHVDSVVADVAAEENA
>Mature_714_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTVNYQERTYAAGRIPGGFFKRE
GRPSEGETLIARLIDRPIRPLFPEGFYNEIQIVATVVSVNPQICPDLVAMIGASAALSLSGVPFNGPIGAARVGFIDDQF
VLNPTMNEQKQSRLDLVVAGTDKAVLMVESEADVLTEEQMLAAVVFGHQQQQVVIDAIKEFTAEAGKPRWDWVAPEPNTA
LIEKVKAIAEARLGEAYRITEKQARYEQIDAIKADVIAQITAEVAEGEDISEGKIVDIFTALESQIVRSRIIAGEPRIDG
RTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGERSDHFLFHYNFPPYSVGETGMIGSPKR
REIGHGRLAKRGVAAVMPTLAEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEDEKFVVL
SDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIEGITAEIMQIALNQAKSARLHILGVMEQAIPAPRADISDFAPRIYTM
KIDPKKIKDVIGKGGATIRALTEETGTSIDIDDDGTVKIAAVDGNSAKEVMARIEDITAEVEAGAVYKGKVTRLADFGAF
VSIVGNKEGLVHISQIAEERVEKVSDYLAVGQEVTVKVVEIDRQGRIRLTMKEVAPKQEHVDSVVADVAAEENA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=706, Percent_Identity=37.1104815864023, Blast_Score=436, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=76.9452449567723, Blast_Score=1105, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=707, Percent_Identity=32.3903818953324, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17535281, Length=76, Percent_Identity=50, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=37.7279102384292, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=37.7279102384292, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=37.7279102384292, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=659, Percent_Identity=37.4810318664643, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PASMU (Q9CLU1)

Other databases:

- EMBL:   AE004439
- EMBL:   AF237935
- RefSeq:   NP_246051.1
- HSSP:   P05055
- ProteinModelPortal:   Q9CLU1
- GeneID:   1244461
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1114
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   PMUL272843:PM1114-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77012; Mature: 77012

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTV
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCEEEEEE
NYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPIRPLFPEGFYNEIQIVATVVSVN
ECCHHEEECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCC
PQICPDLVAMIGASAALSLSGVPFNGPIGAARVGFIDDQFVLNPTMNEQKQSRLDLVVAG
CCCCHHHHHHHCCCHHEEECCCCCCCCCCCCEEEEECCCEEECCCCCHHHHCCEEEEEEC
TDKAVLMVESEADVLTEEQMLAAVVFGHQQQQVVIDAIKEFTAEAGKPRWDWVAPEPNTA
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LIEKVKAIAEARLGEAYRITEKQARYEQIDAIKADVIAQITAEVAEGEDISEGKIVDIFT
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHH
ALESQIVRSRIIAGEPRIDGRTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGT
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQIIDELTGERSDHFLFHYNFPPYSVGETGMIGSPKRREIGHGRLAKRGVAAVMPTL
CCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHH
AEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEDEKFVVL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHCCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIEGITAEIMQIALNQAKSARLHILGVME
EHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCEEEEEEHHH
QAIPAPRADISDFAPRIYTMKIDPKKIKDVIGKGGATIRALTEETGTSIDIDDDGTVKIA
HHCCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
AVDGNSAKEVMARIEDITAEVEAGAVYKGKVTRLADFGAFVSIVGNKEGLVHISQIAEER
EECCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCEEEEECCCCCCEEHHHHHHHH
VEKVSDYLAVGQEVTVKVVEIDRQGRIRLTMKEVAPKQEHVDSVVADVAAEENA
HHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTV
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCEEEEEE
NYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPIRPLFPEGFYNEIQIVATVVSVN
ECCHHEEECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCC
PQICPDLVAMIGASAALSLSGVPFNGPIGAARVGFIDDQFVLNPTMNEQKQSRLDLVVAG
CCCCHHHHHHHCCCHHEEECCCCCCCCCCCCEEEEECCCEEECCCCCHHHHCCEEEEEEC
TDKAVLMVESEADVLTEEQMLAAVVFGHQQQQVVIDAIKEFTAEAGKPRWDWVAPEPNTA
CCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LIEKVKAIAEARLGEAYRITEKQARYEQIDAIKADVIAQITAEVAEGEDISEGKIVDIFT
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHH
ALESQIVRSRIIAGEPRIDGRTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGT
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQIIDELTGERSDHFLFHYNFPPYSVGETGMIGSPKRREIGHGRLAKRGVAAVMPTL
CCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHH
AEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEDEKFVVL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHCCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRTGVTALQMDIKIEGITAEIMQIALNQAKSARLHILGVME
EHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCEEEEEEHHH
QAIPAPRADISDFAPRIYTMKIDPKKIKDVIGKGGATIRALTEETGTSIDIDDDGTVKIA
HHCCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
AVDGNSAKEVMARIEDITAEVEAGAVYKGKVTRLADFGAFVSIVGNKEGLVHISQIAEER
EECCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCEEEEECCCCCCEEHHHHHHHH
VEKVSDYLAVGQEVTVKVVEIDRQGRIRLTMKEVAPKQEHVDSVVADVAAEENA
HHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100; 10873488