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The map label for this gene is ivlE [H]

Identifier: 15602431

GI number: 15602431

Start: 650809

End: 651831

Strand: Reverse

Name: ivlE [H]

Synonym: PM0566

Alternate gene names: 15602431

Gene position: 651831-650809 (Counterclockwise)

Preceding gene: 15602434

Following gene: 161723314

Centisome position: 28.87

GC content: 42.33

Gene sequence:

>1023_bases
ATGATAAAAGATCTTGATTGGAAAAATTTAGGCTTTGGCTATATTAAAACAGATTATCGCTATATCGCTTATTGGAAAGA
CGGAAAGTGGTCAAAAGGCGAGCTGACACAAGATAATGTACTGCATATTAGTGAAGGTTCAACTGCACTTCATTATGGGC
AACAATGCTTTGAAGGACTCAAAGCTTATCGCTGTAAAGACGGTTCGATTAACTTGTTCCGCCCTGATCAAAATGCAGAA
CGTATGCAACGCAGTTGTCGCCGTTTATTGATGCCTGAAGTGCCAACAGACATGTTTATTGATGCCTGTAAACAAGTGGT
CAAAGCAAATCAAGCATGGTTAGCGCCTTATGGCACTGGTGCCACCCTCTATTTACGCCCTTTCTTGATTGGTGTAGGTG
ACAATGTCGGCGTCAATCCTGCGCCAGAATATATTTTCTCGATTTTCTGCTGCCCTGTTGGCGCTTATTTTAAAGGTGGC
TTAAAACCGACCAATTTCTTAGTTTCAAATTATGATCGCGCCGCACCGCAAGGAACCGGGGCAGCAAAAGTGGGTGGAAA
TTACGCAGCAAGTCTTTATCCTGGTAAAGAAGCCAAACAAAACGGCTTTGGTGACTGTATTTATTTAGACCCGACCACAC
ACACCAAAATTGAAGAAGTGGGGTCAGCGAATTTCTTCGGTATCACAAAAGATAACAAATTTATTACTCCGCTTTCGCCG
TCAATTCTGCCAAGTATCACGAAATACTCGTTACTCTATCTCGCTAAAGCGCGGTTAGGGTTAGAAACAGAAGAAGGGGA
TGTGTATATCGATCAATTAGATCAATTTAAAGAAGCGGGCGCTTGTGGTACTGCTGCCGTCATCACACCGATTGGTGGTA
TTCAATATGGTGATAACTTCCATGTGTTCTACTCTGAAACGGAAGTCGGTGAAATCACCACCAAACTCTACAATGAATTA
GTGGGTATCCAATTTGGTGATGTTGAAGCCCCTGAAGGTTGGATTGTTAAAGTAGATAACTAA

Upstream 100 bases:

>100_bases
TTTTTCTTTAATCTTAATCTTTTTTTCAGATAAAGACTTGCAAGCCATGAAGAATGGTGTAGATTAAAAACATCACCAAA
CACAATAAAAGGACATAATA

Downstream 100 bases:

>100_bases
CCCTTAGCAAAGAAACATAAGTGCGGTCGAAAACTTCAAGATTTTCGACCGCACTTTTATGTCTTATTGATGAGATATCA
TTACACTATATTTAGCTGTA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MIKDLDWKNLGFGYIKTDYRYIAYWKDGKWSKGELTQDNVLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAE
RMQRSCRRLLMPEVPTDMFIDACKQVVKANQAWLAPYGTGATLYLRPFLIGVGDNVGVNPAPEYIFSIFCCPVGAYFKGG
LKPTNFLVSNYDRAAPQGTGAAKVGGNYAASLYPGKEAKQNGFGDCIYLDPTTHTKIEEVGSANFFGITKDNKFITPLSP
SILPSITKYSLLYLAKARLGLETEEGDVYIDQLDQFKEAGACGTAAVITPIGGIQYGDNFHVFYSETEVGEITTKLYNEL
VGIQFGDVEAPEGWIVKVDN

Sequences:

>Translated_340_residues
MIKDLDWKNLGFGYIKTDYRYIAYWKDGKWSKGELTQDNVLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAE
RMQRSCRRLLMPEVPTDMFIDACKQVVKANQAWLAPYGTGATLYLRPFLIGVGDNVGVNPAPEYIFSIFCCPVGAYFKGG
LKPTNFLVSNYDRAAPQGTGAAKVGGNYAASLYPGKEAKQNGFGDCIYLDPTTHTKIEEVGSANFFGITKDNKFITPLSP
SILPSITKYSLLYLAKARLGLETEEGDVYIDQLDQFKEAGACGTAAVITPIGGIQYGDNFHVFYSETEVGEITTKLYNEL
VGIQFGDVEAPEGWIVKVDN
>Mature_340_residues
MIKDLDWKNLGFGYIKTDYRYIAYWKDGKWSKGELTQDNVLHISEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAE
RMQRSCRRLLMPEVPTDMFIDACKQVVKANQAWLAPYGTGATLYLRPFLIGVGDNVGVNPAPEYIFSIFCCPVGAYFKGG
LKPTNFLVSNYDRAAPQGTGAAKVGGNYAASLYPGKEAKQNGFGDCIYLDPTTHTKIEEVGSANFFGITKDNKFITPLSP
SILPSITKYSLLYLAKARLGLETEEGDVYIDQLDQFKEAGACGTAAVITPIGGIQYGDNFHVFYSETEVGEITTKLYNEL
VGIQFGDVEAPEGWIVKVDN

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=340, Percent_Identity=33.8235294117647, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI296010906, Length=340, Percent_Identity=33.8235294117647, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI296010904, Length=340, Percent_Identity=33.8235294117647, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI50658084, Length=331, Percent_Identity=31.7220543806647, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI258614015, Length=286, Percent_Identity=32.1678321678322, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI296010902, Length=340, Percent_Identity=28.8235294117647, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI296010900, Length=340, Percent_Identity=28.8235294117647, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI48994963, Length=323, Percent_Identity=32.5077399380805, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17568601, Length=353, Percent_Identity=32.0113314447592, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17565728, Length=352, Percent_Identity=29.2613636363636, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322608, Length=341, Percent_Identity=32.5513196480938, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322002, Length=343, Percent_Identity=30.9037900874636, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24641779, Length=348, Percent_Identity=31.3218390804598, Blast_Score=165, Evalue=4e-41,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37592; Mature: 37592

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDLDWKNLGFGYIKTDYRYIAYWKDGKWSKGELTQDNVLHISEGSTALHYGQQCFEGL
CCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KAYRCKDGSINLFRPDQNAERMQRSCRRLLMPEVPTDMFIDACKQVVKANQAWLAPYGTG
HHEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
ATLYLRPFLIGVGDNVGVNPAPEYIFSIFCCPVGAYFKGGLKPTNFLVSNYDRAAPQGTG
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCCCCCCCC
AAKVGGNYAASLYPGKEAKQNGFGDCIYLDPTTHTKIEEVGSANFFGITKDNKFITPLSP
CEECCCCEEEEECCCCHHCCCCCCCEEEECCCCCCHHHHCCCCCEEEEECCCCEECCCCC
SILPSITKYSLLYLAKARLGLETEEGDVYIDQLDQFKEAGACGTAAVITPIGGIQYGDNF
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCEEEEECCCCEEECCEE
HVFYSETEVGEITTKLYNELVGIQFGDVEAPEGWIVKVDN
EEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCEEEEECC
>Mature Secondary Structure
MIKDLDWKNLGFGYIKTDYRYIAYWKDGKWSKGELTQDNVLHISEGSTALHYGQQCFEGL
CCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KAYRCKDGSINLFRPDQNAERMQRSCRRLLMPEVPTDMFIDACKQVVKANQAWLAPYGTG
HHEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
ATLYLRPFLIGVGDNVGVNPAPEYIFSIFCCPVGAYFKGGLKPTNFLVSNYDRAAPQGTG
CEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCCCCCCCCCC
AAKVGGNYAASLYPGKEAKQNGFGDCIYLDPTTHTKIEEVGSANFFGITKDNKFITPLSP
CEECCCCEEEEECCCCHHCCCCCCCEEEECCCCCCHHHHCCCCCEEEEECCCCEECCCCC
SILPSITKYSLLYLAKARLGLETEEGDVYIDQLDQFKEAGACGTAAVITPIGGIQYGDNF
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCEEEEECCCCEEECCEE
HVFYSETEVGEITTKLYNELVGIQFGDVEAPEGWIVKVDN
EEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]