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The map label for this gene is ldhA [C]

Identifier: 15602424

GI number: 15602424

Start: 642590

End: 643540

Strand: Direct

Name: ldhA [C]

Synonym: PM0559

Alternate gene names: 15602424

Gene position: 642590-643540 (Clockwise)

Preceding gene: 15602423

Following gene: 15602425

Centisome position: 28.46

GC content: 41.64

Gene sequence:

>951_bases
ATGTTAAAAATTGTTTTTCTAGATAGTACCGCGATACCTAAACATATCCCGATTCCTCGCCCAAGTTTTCCACATCAATG
GGTTGAATATGAGCATACGACACCAGATCAAGTCGTAGAACGTATGCAAGATGTAGACATTGCGGTAACCAGTAAAGTCC
TCTTTAGTCGTGAAGTGATGCAACAATTACCTAAATTAAAATTAATCGCGATTACGGCAACAGGGACCAATAATGTGGAT
TTGGTGGCTGCACAAGAACTTGGAATCACGGTGAAAAATGTGACAGGGTATTCATCTACAACCGTACCAGAACATGTGAT
AGGTCTGATATATGCGCTCAAACACAGTATTATGAGCTGGTATCGCGATCAATTATCTGCAAAATGGGCAGATTGCAAAC
AATTTTGTTACTTTGATTATCCGATTACAGATGTGAAAGGCTCAACCCTTGGTGTCGTGGGACGAGGCTGTTTAGGCTCT
GAAATCGGTCGATTAGCGACCGCACTTGGCATGAATGTACTTTATGCAGAACATAAAGGTGCGAAAACATGTCGTGAGGG
TTATACCCCTTTTGAAGACGTGTTAGCACAAGCGGATATCTTGACATTACATTGCCCATTGACGGACACCACACAAAATC
TGATCAATCAAGACACGTTAGCTTTGATGAAAAAAGGCGCATTTTTAATTAATACAGGGCGTGGTCCTTTAGTGGATGAA
CAGGCGTTAGTCGCTGCATTAGAAAGTGGACATCTTGGTGGTGCCGCGGTTGATGTGTTAGTGAAAGAACCGCCTGAAAA
AAACAACTCAATTATCCAAGCTGCCACACGTTTGCCTAATTTAATTGTTACCCCGCATATTGCTTGGGCATCTGACAGTG
CGGTTACCACCTTAGTGAATAAGGTGAAACAAAACATTGAAGACTTTGTAAAAAATGAGCAGATTGCTTAA

Upstream 100 bases:

>100_bases
TTGAATGTATTGCAAAAATTTATTATTGCGCAAATTTCTATTTGCTATCTGAAATTAAATTGGACAGAATAGAAATGCTG
ATAACAAAAAGGAGTGAATA

Downstream 100 bases:

>100_bases
CTAAATTAAATATCGTGATGTTGTTGTATGTTGTTTATGCAATAATAGCACGATATTTTTTATGACTTGATTTTGGTAAA
AATGAAATTTTCTGTTCCTT

Product: glycerate dehydrogenase

Products: H+; Nicotinamide adenine dinucleotide - reduced; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLKIVFLDSTAIPKHIPIPRPSFPHQWVEYEHTTPDQVVERMQDVDIAVTSKVLFSREVMQQLPKLKLIAITATGTNNVD
LVAAQELGITVKNVTGYSSTTVPEHVIGLIYALKHSIMSWYRDQLSAKWADCKQFCYFDYPITDVKGSTLGVVGRGCLGS
EIGRLATALGMNVLYAEHKGAKTCREGYTPFEDVLAQADILTLHCPLTDTTQNLINQDTLALMKKGAFLINTGRGPLVDE
QALVAALESGHLGGAAVDVLVKEPPEKNNSIIQAATRLPNLIVTPHIAWASDSAVTTLVNKVKQNIEDFVKNEQIA

Sequences:

>Translated_316_residues
MLKIVFLDSTAIPKHIPIPRPSFPHQWVEYEHTTPDQVVERMQDVDIAVTSKVLFSREVMQQLPKLKLIAITATGTNNVD
LVAAQELGITVKNVTGYSSTTVPEHVIGLIYALKHSIMSWYRDQLSAKWADCKQFCYFDYPITDVKGSTLGVVGRGCLGS
EIGRLATALGMNVLYAEHKGAKTCREGYTPFEDVLAQADILTLHCPLTDTTQNLINQDTLALMKKGAFLINTGRGPLVDE
QALVAALESGHLGGAAVDVLVKEPPEKNNSIIQAATRLPNLIVTPHIAWASDSAVTTLVNKVKQNIEDFVKNEQIA
>Mature_316_residues
MLKIVFLDSTAIPKHIPIPRPSFPHQWVEYEHTTPDQVVERMQDVDIAVTSKVLFSREVMQQLPKLKLIAITATGTNNVD
LVAAQELGITVKNVTGYSSTTVPEHVIGLIYALKHSIMSWYRDQLSAKWADCKQFCYFDYPITDVKGSTLGVVGRGCLGS
EIGRLATALGMNVLYAEHKGAKTCREGYTPFEDVLAQADILTLHCPLTDTTQNLINQDTLALMKKGAFLINTGRGPLVDE
QALVAALESGHLGGAAVDVLVKEPPEKNNSIIQAATRLPNLIVTPHIAWASDSAVTTLVNKVKQNIEDFVKNEQIA

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=262, Percent_Identity=31.6793893129771, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557497, Length=262, Percent_Identity=31.6793893129771, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI23308577, Length=295, Percent_Identity=29.1525423728814, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI145580578, Length=250, Percent_Identity=33.6, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI4557499, Length=250, Percent_Identity=33.6, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI145580575, Length=250, Percent_Identity=33.2, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI6912396, Length=253, Percent_Identity=25.6916996047431, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787645, Length=272, Percent_Identity=32.3529411764706, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789279, Length=262, Percent_Identity=31.2977099236641, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87082289, Length=271, Percent_Identity=27.6752767527675, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081824, Length=216, Percent_Identity=27.3148148148148, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=34.2629482071713, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=263, Percent_Identity=30.4182509505703, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=297, Percent_Identity=30.976430976431, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320925, Length=295, Percent_Identity=30.8474576271186, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324964, Length=258, Percent_Identity=28.6821705426357, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=219, Percent_Identity=31.9634703196347, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=79, Percent_Identity=37.9746835443038, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325144, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24646446, Length=278, Percent_Identity=34.1726618705036, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24646448, Length=278, Percent_Identity=34.1726618705036, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24646452, Length=278, Percent_Identity=34.1726618705036, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24646450, Length=278, Percent_Identity=34.1726618705036, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI62472511, Length=278, Percent_Identity=34.1726618705036, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921140, Length=290, Percent_Identity=30.6896551724138, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574286, Length=249, Percent_Identity=27.710843373494, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45552429, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585514, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28574282, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28574284, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45551003, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24585516, Length=230, Percent_Identity=24.7826086956522, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571528, Length=265, Percent_Identity=25.6603773584906, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28 [C]

Molecular weight: Translated: 34600; Mature: 34600

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIVFLDSTAIPKHIPIPRPSFPHQWVEYEHTTPDQVVERMQDVDIAVTSKVLFSREVM
CEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
QQLPKLKLIAITATGTNNVDLVAAQELGITVKNVTGYSSTTVPEHVIGLIYALKHSIMSW
HHCCCEEEEEEEECCCCCEEEEEEHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
YRDQLSAKWADCKQFCYFDYPITDVKGSTLGVVGRGCLGSEIGRLATALGMNVLYAEHKG
HHHHHHCCHHHHHHHEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCHHEEECCCC
AKTCREGYTPFEDVLAQADILTLHCPLTDTTQNLINQDTLALMKKGAFLINTGRGPLVDE
HHHHHHCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCH
QALVAALESGHLGGAAVDVLVKEPPEKNNSIIQAATRLPNLIVTPHIAWASDSAVTTLVN
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEECCCEECCCCHHHHHHHH
KVKQNIEDFVKNEQIA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKIVFLDSTAIPKHIPIPRPSFPHQWVEYEHTTPDQVVERMQDVDIAVTSKVLFSREVM
CEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
QQLPKLKLIAITATGTNNVDLVAAQELGITVKNVTGYSSTTVPEHVIGLIYALKHSIMSW
HHCCCEEEEEEEECCCCCEEEEEEHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
YRDQLSAKWADCKQFCYFDYPITDVKGSTLGVVGRGCLGSEIGRLATALGMNVLYAEHKG
HHHHHHCCHHHHHHHEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCHHEEECCCC
AKTCREGYTPFEDVLAQADILTLHCPLTDTTQNLINQDTLALMKKGAFLINTGRGPLVDE
HHHHHHCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCH
QALVAALESGHLGGAAVDVLVKEPPEKNNSIIQAATRLPNLIVTPHIAWASDSAVTTLVN
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEECCCEECCCCHHHHHHHH
KVKQNIEDFVKNEQIA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Lactate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Lactate + Nicotinamide adenine dinucleotide <==> H+ + Nicotinamide adenine dinucleotide - reduced + Pyruvate [C]

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]