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The map label for this gene is kdsA

Identifier: 15602423

GI number: 15602423

Start: 641553

End: 642407

Strand: Direct

Name: kdsA

Synonym: PM0558

Alternate gene names: 15602423

Gene position: 641553-642407 (Clockwise)

Preceding gene: 15602422

Following gene: 15602424

Centisome position: 28.42

GC content: 40.58

Gene sequence:

>855_bases
ATGCAAAATAAAGTGATTCGCTTAGGCGAAATTGAGATTTCTAATGATAAACCCTTTGTTTTGTTTGGGGGAATGAACGT
ATTAGAAAGTCGCGATATGGCGATGCAAGTGTGCGAAGCTTATGTTAAGGTCACGGAAAAATTAGGGGTGCCTTATGTGT
TTAAGGCTTCTTTTGACAAAGCAAACCGCTCATCTATCCATTCTTATCGTGGACCGGGTATGGAACAAGGTCTGAAAATC
TTCCAAGAGTTGAAAGACACATTTGGGGTTAAATTGATTACAGATGTACATGAAATTTATCAATGTCAACCAGTCGCTGA
AGTTGTTGATATCATTCAATTACCTGCTTTTTTAGCACGTCAAACCGATTTAGTGGCAGCGATGGCAAAAACGGGCGCGA
TTATTAATGTGAAAAAACCACAATTTTTAAGTCCCGGTCAAATGGGGAATATCGTTGAGAAAATTGAAGAATGTGGTAAT
GATAACGTTATTTTATGCGATCGAGGCACCAATTTTGGTTATGACAATTTAGTTGTAGATATGTTGGGTTTTTCTGTAAT
GAAAAAAGTATCAAAAGGTTGCCCTGTGATTTTTGATGTGACCCACGCATTACAATGTCGTGATCCATTTGGTGCGGCCT
CAGGGGGACGTCGTGGGCAAGTCACGGAATTAGCGCGCGCGGGAATGGCAGTGGGATTAGCCGGGTTATTTTTAGAAGCA
CATCCGAATCCGAGTCAAGCGAAATGTGATGGGCCTTCAGCTTTACCGCTATCTGCATTAGAAGGTTTTGTACATCAAAT
GAAAGCGATTGATGATCTGGTGAAACATTTCCCAGAATTAGATACTGCCAATTAA

Upstream 100 bases:

>100_bases
ATCGATCAATGCCCTCAGGACCCAACCGCGCTTTTACTGAAAAATCAGTTAGAAGAGTTAAAACAAGATACTTATCCTAT
ACATTAAGAAGGAAAACATG

Downstream 100 bases:

>100_bases
TTTGTGTGAAAAACGAAAGCCCTGCCTTTTCTGAGGTAGGGCTTTTTTATATGTCTATATTATTCATAAAAGTCACTTAT
TTTTGAATGTATTGCAAAAA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQNKVIRLGEIEISNDKPFVLFGGMNVLESRDMAMQVCEAYVKVTEKLGVPYVFKASFDKANRSSIHSYRGPGMEQGLKI
FQELKDTFGVKLITDVHEIYQCQPVAEVVDIIQLPAFLARQTDLVAAMAKTGAIINVKKPQFLSPGQMGNIVEKIEECGN
DNVILCDRGTNFGYDNLVVDMLGFSVMKKVSKGCPVIFDVTHALQCRDPFGAASGGRRGQVTELARAGMAVGLAGLFLEA
HPNPSQAKCDGPSALPLSALEGFVHQMKAIDDLVKHFPELDTAN

Sequences:

>Translated_284_residues
MQNKVIRLGEIEISNDKPFVLFGGMNVLESRDMAMQVCEAYVKVTEKLGVPYVFKASFDKANRSSIHSYRGPGMEQGLKI
FQELKDTFGVKLITDVHEIYQCQPVAEVVDIIQLPAFLARQTDLVAAMAKTGAIINVKKPQFLSPGQMGNIVEKIEECGN
DNVILCDRGTNFGYDNLVVDMLGFSVMKKVSKGCPVIFDVTHALQCRDPFGAASGGRRGQVTELARAGMAVGLAGLFLEA
HPNPSQAKCDGPSALPLSALEGFVHQMKAIDDLVKHFPELDTAN
>Mature_284_residues
MQNKVIRLGEIEISNDKPFVLFGGMNVLESRDMAMQVCEAYVKVTEKLGVPYVFKASFDKANRSSIHSYRGPGMEQGLKI
FQELKDTFGVKLITDVHEIYQCQPVAEVVDIIQLPAFLARQTDLVAAMAKTGAIINVKKPQFLSPGQMGNIVEKIEECGN
DNVILCDRGTNFGYDNLVVDMLGFSVMKKVSKGCPVIFDVTHALQCRDPFGAASGGRRGQVTELARAGMAVGLAGLFLEA
HPNPSQAKCDGPSALPLSALEGFVHQMKAIDDLVKHFPELDTAN

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=283, Percent_Identity=80.565371024735, Blast_Score=483, Evalue=1e-138,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_PASMU (P57853)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245495.1
- ProteinModelPortal:   P57853
- SMR:   P57853
- PRIDE:   P57853
- GeneID:   1243905
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0558
- NMPDR:   fig|272843.1.peg.558
- HOGENOM:   HBG575990
- OMA:   VHEPHQA
- ProtClustDB:   PRK05198
- BioCyc:   PMUL272843:PM0558-MONOMER
- BRENDA:   2.5.1.55
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30949; Mature: 30949

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKVIRLGEIEISNDKPFVLFGGMNVLESRDMAMQVCEAYVKVTEKLGVPYVFKASFDK
CCCCEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSIHSYRGPGMEQGLKIFQELKDTFGVKLITDVHEIYQCQPVAEVVDIIQLPAFLAR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QTDLVAAMAKTGAIINVKKPQFLSPGQMGNIVEKIEECGNDNVILCDRGTNFGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
MLGFSVMKKVSKGCPVIFDVTHALQCRDPFGAASGGRRGQVTELARAGMAVGLAGLFLEA
HHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
HPNPSQAKCDGPSALPLSALEGFVHQMKAIDDLVKHFPELDTAN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQNKVIRLGEIEISNDKPFVLFGGMNVLESRDMAMQVCEAYVKVTEKLGVPYVFKASFDK
CCCCEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSIHSYRGPGMEQGLKIFQELKDTFGVKLITDVHEIYQCQPVAEVVDIIQLPAFLAR
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QTDLVAAMAKTGAIINVKKPQFLSPGQMGNIVEKIEECGNDNVILCDRGTNFGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
MLGFSVMKKVSKGCPVIFDVTHALQCRDPFGAASGGRRGQVTELARAGMAVGLAGLFLEA
HHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
HPNPSQAKCDGPSALPLSALEGFVHQMKAIDDLVKHFPELDTAN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100