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The map label for this gene is purR

Identifier: 15602412

GI number: 15602412

Start: 631172

End: 632176

Strand: Reverse

Name: purR

Synonym: PM0547

Alternate gene names: 15602412

Gene position: 632176-631172 (Counterclockwise)

Preceding gene: 15602413

Following gene: 15602411

Centisome position: 28.0

GC content: 41.0

Gene sequence:

>1005_bases
ATGGCAACAATTAAAGATGTCGCAAAAATGGCTGGGGTGTCCACCACCACAGTTTCGCATGTAATTAATAAAACCCGCTT
TGTTGCAGCTGAAACAGAAAAACTGGTATTGCAAGCAATTCAAGAACTCAACTATTCCCCTAGTGCGGTCGCACGTAGCT
TAAAAGTCAATACGACAAAATCGATTGGCATGATTGTCACCAGTAGTGAAGCCCCTTATTTTGCAGAAATCATTCACGCG
GTGGAAGAACATTGCTATCGCCAAGGTTACTCACTGTTTTTATGTAATACACAAAATAACGTGGAAAAAATTAAAAACCA
CCTTGAAATGCTGATTAAAAAACGCGTCGATGGCATCTTAGTCATGTGTGCAGAATATACTCAAGATTCACTCGATTTAT
TAGCGAATTTCACCTCTTTGCCACTGGTCGTGATGGACTGGGGACCAGACAATAAACATACTGATATCATTCAAGATCAT
AGTTTTGAGGGCGGTTATCTTGCCACAAAATATTTAATTGATCACGGGCATAAAGATATCGGTATTATTGCAGGTGAGCT
TACGAAAACGACAGCCAAAACACGCTATGAAGGTTTCATTAAAGCGATGAATGAAGCAGGGCTAAAGATTAACCCTGATT
GGGTGATGGAGGGATTCTTTGAGCCTGAGGATGGTTATGAGTGCATGAATAAAATCTTGGCACAAGACACTCTCCCGACG
GCTGTCTTTTGCTGTAATGATGTTATGGCGTTAGGGGCAATTTCCGCAATCGGTGAAAAAGGTTTACGTGTGCCAGAAGA
TATTTCAATTATTGGTTATGATAATATTCACGCTTCACGCTTTTACTCCCCGCCTTTAACCACCATACACCAATCAAAAT
CCCGTTTAGGTGCTCGTGCGGTGACTTTATTATTCGAACGCATTAATGAAAAATCTGAAGAGCGTGCCGTCATCGAAATG
CATCCTGAACTTGTCATCCGTAAATCAGTCAAATCTCGCCTTTAA

Upstream 100 bases:

>100_bases
AATGAAAATTGTGATGTTAGACAAATATTTCTCATCTATTACTTAACCAATTGTCATTCACTCTTTATAATGAAATCCAT
TTGTATGGAGAAAAAGAATT

Downstream 100 bases:

>100_bases
CCTCCATAATGCCCTTATTTTTTCACAATATAAGGGCATTAATATTTAACGCCATTCATTAAAAAATATTCAATAAAAAA
TCTAAAAAATTGATTAAAAA

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELNYSPSAVARSLKVNTTKSIGMIVTSSEAPYFAEIIHA
VEEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGILVMCAEYTQDSLDLLANFTSLPLVVMDWGPDNKHTDIIQDH
SFEGGYLATKYLIDHGHKDIGIIAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYECMNKILAQDTLPT
AVFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARAVTLLFERINEKSEERAVIEM
HPELVIRKSVKSRL

Sequences:

>Translated_334_residues
MATIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELNYSPSAVARSLKVNTTKSIGMIVTSSEAPYFAEIIHA
VEEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGILVMCAEYTQDSLDLLANFTSLPLVVMDWGPDNKHTDIIQDH
SFEGGYLATKYLIDHGHKDIGIIAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYECMNKILAQDTLPT
AVFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARAVTLLFERINEKSEERAVIEM
HPELVIRKSVKSRL
>Mature_333_residues
ATIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELNYSPSAVARSLKVNTTKSIGMIVTSSEAPYFAEIIHAV
EEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGILVMCAEYTQDSLDLLANFTSLPLVVMDWGPDNKHTDIIQDHS
FEGGYLATKYLIDHGHKDIGIIAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYECMNKILAQDTLPTA
VFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARAVTLLFERINEKSEERAVIEMH
PELVIRKSVKSRL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=57.4018126888217, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=47.112462006079, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=35.2238805970149, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=35.3951890034364, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=29.4294294294294, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786540, Length=305, Percent_Identity=34.0983606557377, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=29.4520547945205, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.6666666666667, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787906, Length=326, Percent_Identity=25.4601226993865, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=29.4670846394984, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=26.6025641025641, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790689, Length=319, Percent_Identity=26.0188087774295, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790715, Length=322, Percent_Identity=23.6024844720497, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786268, Length=348, Percent_Identity=21.551724137931, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_PASMU (Q9CN88)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245484.1
- ProteinModelPortal:   Q9CN88
- SMR:   Q9CN88
- GeneID:   1243894
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0547
- NMPDR:   fig|272843.1.peg.547
- HOGENOM:   HBG753640
- OMA:   IKNHLEM
- ProtClustDB:   PRK10703
- BioCyc:   PMUL272843:PM0547-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37244; Mature: 37113

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 189-189 BINDING 191-191 BINDING 220-220 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELNYSPSAVARSLKVNTTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIVTSSEAPYFAEIIHAVEEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGIL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEE
VMCAEYTQDSLDLLANFTSLPLVVMDWGPDNKHTDIIQDHSFEGGYLATKYLIDHGHKDI
EEEHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCE
GIIAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYECMNKILAQDTLPT
EEEECHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHCCCCCCCCCHHHHHHHHHHCCCCH
AVFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
VTLLFERINEKSEERAVIEMHPELVIRKSVKSRL
HHHHHHHHCCHHHCCEEEHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ATIKDVAKMAGVSTTTVSHVINKTRFVAAETEKLVLQAIQELNYSPSAVARSLKVNTTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIVTSSEAPYFAEIIHAVEEHCYRQGYSLFLCNTQNNVEKIKNHLEMLIKKRVDGIL
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEE
VMCAEYTQDSLDLLANFTSLPLVVMDWGPDNKHTDIIQDHSFEGGYLATKYLIDHGHKDI
EEEHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCE
GIIAGELTKTTAKTRYEGFIKAMNEAGLKINPDWVMEGFFEPEDGYECMNKILAQDTLPT
EEEECHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHCCCCCCCCCHHHHHHHHHHCCCCH
AVFCCNDVMALGAISAIGEKGLRVPEDISIIGYDNIHASRFYSPPLTTIHQSKSRLGARA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
VTLLFERINEKSEERAVIEMHPELVIRKSVKSRL
HHHHHHHHCCHHHCCEEEHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100