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The map label for this gene is murE

Identifier: 15594546

GI number: 15594546

Start: 201052

End: 202578

Strand: Reverse

Name: murE

Synonym: BB0201

Alternate gene names: 15594546

Gene position: 202578-201052 (Counterclockwise)

Preceding gene: 15594551

Following gene: 15594545

Centisome position: 22.24

GC content: 30.39

Gene sequence:

>1527_bases
ATGAATAAAAAACTTAATGAAGTTTTATTAAAGTTAGATCAAGATTTAATAAAATGTGTAAAAGGTTCTCTTGATTTAGA
AATATCAGGAGTTACTTATAGTTCTAAATTGGTTTTGCCCAGGTTTGTGTTTTTTGCTCTTCCAGGAATTCATTTTGATG
GGCATGATTTTATTGAAATTGCAATTCAAAAGGGTAGTAATGTTGTTGTGTGTTCACGAGATGTGGATTTTTACAGTCCT
AATGTTACTTATATTAAGGTAGATGACTTTAACATAAGAAAATTTATGTCTAATTTTTCAAATATTTTTTATGATGAGCC
TTCAAAAAAATTAAAAGTTATTGGAGTCACTGGCACTGACGGGAAAAGTTCTGTTTGTTATTATATATATCTTCTTTTTA
AAAAAAAGGGTGTTAAAGTAGGTTTTATATCGACAGTATTTTTTGATGATGGGAGTGGAAGCTTGATTAAAAATCCTTAC
AGACAATCAACTCCCGAGTCTACGGAAATACATTCATTTTTAAGCACCATGGTTAAAAATGAAGCTCAATATGCAATTCT
TGAATCTACTTCTCATGGGCTTGACCTTGAAACAGCAAGGCTTATTGATGTTAATTATTTTGCAGTTGTTTTTACCAATA
TTGGACATGAGCATCTTGAATTTCATGGCACAATTCAAAATTATTTGAATGTCAAGCTGGGTCTTTTTCGGTCTGTTAGT
GATGATGCTGGTTTTGGGGTTATTAATCTTGATGACCTTTATTCTTCTGATTTTAAGAATGCTGTTAAGAAATCTTTTAC
TTATAGCTTAAAAAGCAGTAAAGCGGATTTTTTTGTTAGTTTTATTGATGAGAAAACCGATTCTACTAGATTTGAATTTT
ATCACAAGGGGGTTAAATATCTTGCTAATGTTAGCCTACTGGGGAGTTTTAATGTTGAGAATGTAATGGCTGCTCTTATT
TTAGTTTCTCAAATTTTAAATATCGATATTCAAGATATTGTTGATAAACTTAACTGCATTAAAAGTCTTGATGGGCGTAT
GGATAGTATTAATTTGGGGCAAAATTTTTCTGTAATAATTGATTATGCTCATACTCCTGGTGCTTTTTCCAAGCTTTTTC
CTATTTTTAAAAGATTTGCTACCAATAGATTGATTTCTGTTTTTGGCTCTGCAGGAGAAAGAGATGTTGAAAAAAGATTT
TTGCAAGGGCAAATCGCAGATATTTATTCTGATTTAATAATACTTTGCGATGAAGATCCAAGAGGCGAGAATAGTATGTG
TATAATTAAAGACATTGCAAAAGGAATTGTAAATAAAGTTGAAAATAAGGATTTATTTTTTATTGCTGATAGAAAGCAGG
CTATTGAAAAAGCAATAAGTCTTGCAAAAGCAGGAGATTTGGTTGTTGCTTTGGGCAAAGGTCATGAAAGTTCAATAATT
TATAAAAATAGAGAAGTTTTTTGGAATGAACAAGAGGTAGTTAAAAATGCTATTTTAAGTTTAGAAAAATCAGAAAAGGA
GAAGTGA

Upstream 100 bases:

>100_bases
AGTTTATTAAAAAAATAGAAAATTGTAAATATATTTTCTATTGAAGCATAATTTTGAATTTCTTAGCTATTTTTATTAGT
TTTATGTAAAATTTTAATGT

Downstream 100 bases:

>100_bases
TTTGAAAAAAAATCTTATGTTGATATTTGGTGGCGTTTCTTTTGAGCATGAAATTTCTTGCAAATCTGCTTATAGTATTT
ATTTAGCCCTTTTGGATCTA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNKKLNEVLLKLDQDLIKCVKGSLDLEISGVTYSSKLVLPRFVFFALPGIHFDGHDFIEIAIQKGSNVVVCSRDVDFYSP
NVTYIKVDDFNIRKFMSNFSNIFYDEPSKKLKVIGVTGTDGKSSVCYYIYLLFKKKGVKVGFISTVFFDDGSGSLIKNPY
RQSTPESTEIHSFLSTMVKNEAQYAILESTSHGLDLETARLIDVNYFAVVFTNIGHEHLEFHGTIQNYLNVKLGLFRSVS
DDAGFGVINLDDLYSSDFKNAVKKSFTYSLKSSKADFFVSFIDEKTDSTRFEFYHKGVKYLANVSLLGSFNVENVMAALI
LVSQILNIDIQDIVDKLNCIKSLDGRMDSINLGQNFSVIIDYAHTPGAFSKLFPIFKRFATNRLISVFGSAGERDVEKRF
LQGQIADIYSDLIILCDEDPRGENSMCIIKDIAKGIVNKVENKDLFFIADRKQAIEKAISLAKAGDLVVALGKGHESSII
YKNREVFWNEQEVVKNAILSLEKSEKEK

Sequences:

>Translated_508_residues
MNKKLNEVLLKLDQDLIKCVKGSLDLEISGVTYSSKLVLPRFVFFALPGIHFDGHDFIEIAIQKGSNVVVCSRDVDFYSP
NVTYIKVDDFNIRKFMSNFSNIFYDEPSKKLKVIGVTGTDGKSSVCYYIYLLFKKKGVKVGFISTVFFDDGSGSLIKNPY
RQSTPESTEIHSFLSTMVKNEAQYAILESTSHGLDLETARLIDVNYFAVVFTNIGHEHLEFHGTIQNYLNVKLGLFRSVS
DDAGFGVINLDDLYSSDFKNAVKKSFTYSLKSSKADFFVSFIDEKTDSTRFEFYHKGVKYLANVSLLGSFNVENVMAALI
LVSQILNIDIQDIVDKLNCIKSLDGRMDSINLGQNFSVIIDYAHTPGAFSKLFPIFKRFATNRLISVFGSAGERDVEKRF
LQGQIADIYSDLIILCDEDPRGENSMCIIKDIAKGIVNKVENKDLFFIADRKQAIEKAISLAKAGDLVVALGKGHESSII
YKNREVFWNEQEVVKNAILSLEKSEKEK
>Mature_508_residues
MNKKLNEVLLKLDQDLIKCVKGSLDLEISGVTYSSKLVLPRFVFFALPGIHFDGHDFIEIAIQKGSNVVVCSRDVDFYSP
NVTYIKVDDFNIRKFMSNFSNIFYDEPSKKLKVIGVTGTDGKSSVCYYIYLLFKKKGVKVGFISTVFFDDGSGSLIKNPY
RQSTPESTEIHSFLSTMVKNEAQYAILESTSHGLDLETARLIDVNYFAVVFTNIGHEHLEFHGTIQNYLNVKLGLFRSVS
DDAGFGVINLDDLYSSDFKNAVKKSFTYSLKSSKADFFVSFIDEKTDSTRFEFYHKGVKYLANVSLLGSFNVENVMAALI
LVSQILNIDIQDIVDKLNCIKSLDGRMDSINLGQNFSVIIDYAHTPGAFSKLFPIFKRFATNRLISVFGSAGERDVEKRF
LQGQIADIYSDLIILCDEDPRGENSMCIIKDIAKGIVNKVENKDLFFIADRKQAIEKAISLAKAGDLVVALGKGHESSII
YKNREVFWNEQEVVKNAILSLEKSEKEK

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=484, Percent_Identity=26.6528925619835, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_BORBU (O51219)

Other databases:

- EMBL:   AE000783
- PIR:   A70125
- RefSeq:   NP_212335.1
- ProteinModelPortal:   O51219
- SMR:   O51219
- EnsemblBacteria:   EBBORT00000008948
- GeneID:   1195036
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0201
- NMPDR:   fig|224326.1.peg.585
- TIGR:   BB_0201
- GeneTree:   EBGT00050000006937
- HOGENOM:   HBG602753
- OMA:   WINATNI
- ProtClustDB:   CLSK507427
- BioCyc:   BBUR224326:BB_0201-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.13

Molecular weight: Translated: 57149; Mature: 57149

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: BINDING 35-35 BINDING 190-190 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLNEVLLKLDQDLIKCVKGSLDLEISGVTYSSKLVLPRFVFFALPGIHFDGHDFIEI
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECEEECCCHHHHHHHHHHCCCCCCCCCCEEEE
AIQKGSNVVVCSRDVDFYSPNVTYIKVDDFNIRKFMSNFSNIFYDEPSKKLKVIGVTGTD
EEECCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHEECCCCCCEEEEEEEECCC
GKSSVCYYIYLLFKKKGVKVGFISTVFFDDGSGSLIKNPYRQSTPESTEIHSFLSTMVKN
CCCCCEEEEEEEEHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
EAQYAILESTSHGLDLETARLIDVNYFAVVFTNIGHEHLEFHGTIQNYLNVKLGLFRSVS
CCCEEEEECCCCCCCCCEEEEEECEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
DDAGFGVINLDDLYSSDFKNAVKKSFTYSLKSSKADFFVSFIDEKTDSTRFEFYHKGVKY
CCCCEEEEEHHHHHHHHHHHHHHHHHEEHHCCCCCCEEEEEHHCCCCCHHHHHHHHHHHH
LANVSLLGSFNVENVMAALILVSQILNIDIQDIVDKLNCIKSLDGRMDSINLGQNFSVII
HHCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEE
DYAHTPGAFSKLFPIFKRFATNRLISVFGSAGERDVEKRFLQGQIADIYSDLIILCDEDP
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCC
RGENSMCIIKDIAKGIVNKVENKDLFFIADRKQAIEKAISLAKAGDLVVALGKGHESSII
CCCCCEEEHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCEE
YKNREVFWNEQEVVKNAILSLEKSEKEK
EECCCEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKKLNEVLLKLDQDLIKCVKGSLDLEISGVTYSSKLVLPRFVFFALPGIHFDGHDFIEI
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECEEECCCHHHHHHHHHHCCCCCCCCCCEEEE
AIQKGSNVVVCSRDVDFYSPNVTYIKVDDFNIRKFMSNFSNIFYDEPSKKLKVIGVTGTD
EEECCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHEECCCCCCEEEEEEEECCC
GKSSVCYYIYLLFKKKGVKVGFISTVFFDDGSGSLIKNPYRQSTPESTEIHSFLSTMVKN
CCCCCEEEEEEEEHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
EAQYAILESTSHGLDLETARLIDVNYFAVVFTNIGHEHLEFHGTIQNYLNVKLGLFRSVS
CCCEEEEECCCCCCCCCEEEEEECEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
DDAGFGVINLDDLYSSDFKNAVKKSFTYSLKSSKADFFVSFIDEKTDSTRFEFYHKGVKY
CCCCEEEEEHHHHHHHHHHHHHHHHHEEHHCCCCCCEEEEEHHCCCCCHHHHHHHHHHHH
LANVSLLGSFNVENVMAALILVSQILNIDIQDIVDKLNCIKSLDGRMDSINLGQNFSVII
HHCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEE
DYAHTPGAFSKLFPIFKRFATNRLISVFGSAGERDVEKRFLQGQIADIYSDLIILCDEDP
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCC
RGENSMCIIKDIAKGIVNKVENKDLFFIADRKQAIEKAISLAKAGDLVVALGKGHESSII
CCCCCEEEHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCEE
YKNREVFWNEQEVVKNAILSLEKSEKEK
EECCCEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685