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The map label for this gene is rsmD [H]

Identifier: 15594551

GI number: 15594551

Start: 208084

End: 208653

Strand: Reverse

Name: rsmD [H]

Synonym: BB0206

Alternate gene names: 15594551

Gene position: 208653-208084 (Counterclockwise)

Preceding gene: 15594558

Following gene: 15594546

Centisome position: 22.91

GC content: 26.49

Gene sequence:

>570_bases
TTGTTTTTTAAAATTGTTTTAAAATTTTTATTTATGTATGTAAGTTCGGGCAAATATAAAGGTAAAAAGATTTTGTTTCC
TAAGAATGGCTCTGTTCGTCCTGTGATGTCTCTTGTTAGAGAAGCCTTTTTTTCTATTATTTTCAAGGATATTGTTAATT
CAAAATTTTTGGATGTTTTTGCAGGGACTGGTATAATGTCTGTTGAGGCTCTTAGCAGGGGGGCTAGTCTTGCTCATCTT
GTTGAATGTAATAGAAAAATTAAAATTACATTAGTTGAGAATTTTAGTTTTGTTGAGGAATTTTATAAATTCTTTTTTCA
AAGAGCAGAGGATTTTTTGAGTAAAAAAGATCTTTTTTATGATTTTATTTATCTTGATCCCCCGTTTAATTATAAAAATA
AGATTAATTTGCTTGAGATTATTTTAAAGGGTAAAATTTTAAATGATAAAGTTAGCATCATTATGCATTATCCTTCTAAT
GAAGATTTGGAGATAAATACATCAAAATTTTCAGTTTATAATTTAAAAAGGTATGGGGGGTCAAAACTTATTTTTTTGAA
AATATTGTAG

Upstream 100 bases:

>100_bases
TGGTAAAAATCTTGTTCCATACCCAGCTGCCAGAATAATGCCTTTCATATTTTTCTCTCTTAAAATAAGATTATAGCAAA
AATTTAATTTTATATATTCA

Downstream 100 bases:

>100_bases
GATTTATTTTTTTTTCATAACTAAAGGGAATGAGTTGAATTTCATTTTTATTTAATTCTGGAATACTGTTTTTTATTACA
AAATCATCCACATTGTTTAA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MFFKIVLKFLFMYVSSGKYKGKKILFPKNGSVRPVMSLVREAFFSIIFKDIVNSKFLDVFAGTGIMSVEALSRGASLAHL
VECNRKIKITLVENFSFVEEFYKFFFQRAEDFLSKKDLFYDFIYLDPPFNYKNKINLLEIILKGKILNDKVSIIMHYPSN
EDLEINTSKFSVYNLKRYGGSKLIFLKIL

Sequences:

>Translated_189_residues
MFFKIVLKFLFMYVSSGKYKGKKILFPKNGSVRPVMSLVREAFFSIIFKDIVNSKFLDVFAGTGIMSVEALSRGASLAHL
VECNRKIKITLVENFSFVEEFYKFFFQRAEDFLSKKDLFYDFIYLDPPFNYKNKINLLEIILKGKILNDKVSIIMHYPSN
EDLEINTSKFSVYNLKRYGGSKLIFLKIL
>Mature_189_residues
MFFKIVLKFLFMYVSSGKYKGKKILFPKNGSVRPVMSLVREAFFSIIFKDIVNSKFLDVFAGTGIMSVEALSRGASLAHL
VECNRKIKITLVENFSFVEEFYKFFFQRAEDFLSKKDLFYDFIYLDPPFNYKNKINLLEIILKGKILNDKVSIIMHYPSN
EDLEINTSKFSVYNLKRYGGSKLIFLKIL

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=128, Percent_Identity=33.59375, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22062; Mature: 22062

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFKIVLKFLFMYVSSGKYKGKKILFPKNGSVRPVMSLVREAFFSIIFKDIVNSKFLDVF
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AGTGIMSVEALSRGASLAHLVECNRKIKITLVENFSFVEEFYKFFFQRAEDFLSKKDLFY
HCCCCEEHHHHHCCCCHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEE
DFIYLDPPFNYKNKINLLEIILKGKILNDKVSIIMHYPSNEDLEINTSKFSVYNLKRYGG
EEEEECCCCCCCCCHHHHHHHHHCEEECCEEEEEEECCCCCCEEEECCEEEEEEEHCCCC
SKLIFLKIL
CEEEEEEEC
>Mature Secondary Structure
MFFKIVLKFLFMYVSSGKYKGKKILFPKNGSVRPVMSLVREAFFSIIFKDIVNSKFLDVF
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AGTGIMSVEALSRGASLAHLVECNRKIKITLVENFSFVEEFYKFFFQRAEDFLSKKDLFY
HCCCCEEHHHHHCCCCHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEE
DFIYLDPPFNYKNKINLLEIILKGKILNDKVSIIMHYPSNEDLEINTSKFSVYNLKRYGG
EEEEECCCCCCCCCHHHHHHHHHCEEECCEEEEEEECCCCCCEEEECCEEEEEEEHCCCC
SKLIFLKIL
CEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]