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The map label for this gene is ddl [H]

Identifier: 15594545

GI number: 15594545

Start: 199965

End: 201035

Strand: Reverse

Name: ddl [H]

Synonym: BB0200

Alternate gene names: 15594545

Gene position: 201035-199965 (Counterclockwise)

Preceding gene: 15594546

Following gene: 15594540

Centisome position: 22.07

GC content: 30.35

Gene sequence:

>1071_bases
ATGTTGATATTTGGTGGCGTTTCTTTTGAGCATGAAATTTCTTGCAAATCTGCTTATAGTATTTATTTAGCCCTTTTGGA
TCTAAATAAATACAATATTTATCCTGTTTATATTGATAAGTGTACAGGTGTTTGGTATTTATTAGATTCTGTTTCTGATC
CTCCCAAGCCCATTAATACAGATGTTTTACCTATTGTTAGTTTATTGCCAGGTTTTGGAATTTTTTCAAACAATAAAAAT
CTTGAAATTGATGTTGTTTTTCCCGTTGTTCACGGAAGAACCGGTGAAGATGGCGCTATTCAAGGGGTTTTAAAGGTTAT
GGACATTCCTTGTGTTGGTGCTGGTATTATAGGGAGCGCCATTTCTAGTAATAAGTATTTTTGCAAACTTTTGCTTAAAA
GTTTTGATATCCCTTTGGTTCCTTTTATTGGATTTAGACAGCATGATTATTTTTTAGACAAAGAAGAAATAAAAAGAAAT
GTAAAGGAGGTTTTAGGCTATCCTGTTATTGTTAAGCCCGCAGTATTAGGGTCTTCAATTGGAATAAATGTAGCTTACAG
CGAAAATCAGATTGAATCTTTTATTAAAGAAGCTTTAAAGTATGACCTTACTATTGTAATAGAGAAGTTTATTGAGGCTA
GAGAAATCGAATGTTCTATTATTGGGAATGAAAAGATGAAAATATTTTCTCCTGGGGAAGTTGTTGTGCAAGATTTTATA
TTTTATGATTATGATGCTAAATATTCTGTTATTCCTGGAAATTCTATTATTTTTAATATTCCTGCACATCTTGAGACAAA
TCAGTTATTAAGCATTAAAGAATATGCTTTTCTTACTTATAAGAATCTAGAACTCAGAGGTATGGCAAGAGTTGATTTTT
TTGTTGAAAAAAAATCAGGAACTATTTATCTTAATGAAATAAACACTATTCCAGGATTTACCGATATATCTATGTTTGCT
AAAATGTGCAGCAATGATGGTCTTCAGTTTAAAGACCTGGTGGATAATTTAATTGATTATGCTTTTCAAAGTTACATTAA
TAGGAAAAAAAGGATTGATTTTGAAAATTAA

Upstream 100 bases:

>100_bases
AAAAATAGAGAAGTTTTTTGGAATGAACAAGAGGTAGTTAAAAATGCTATTTTAAGTTTAGAAAAATCAGAAAAGGAGAA
GTGATTTGAAAAAAAATCTT

Downstream 100 bases:

>100_bases
TTTAGATTATTAGATTAATTTAATTTTAAATTTTTTAGATTTTCAATCTCTTTATCATCTTTTAGTTTAAAGTATTCAAG
ATTTTTATATTTTTCTGCTT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLIFGGVSFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINTDVLPIVSLLPGFGIFSNNKN
LEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSAISSNKYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIKRN
VKEVLGYPVIVKPAVLGSSIGINVAYSENQIESFIKEALKYDLTIVIEKFIEAREIECSIIGNEKMKIFSPGEVVVQDFI
FYDYDAKYSVIPGNSIIFNIPAHLETNQLLSIKEYAFLTYKNLELRGMARVDFFVEKKSGTIYLNEINTIPGFTDISMFA
KMCSNDGLQFKDLVDNLIDYAFQSYINRKKRIDFEN

Sequences:

>Translated_356_residues
MLIFGGVSFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINTDVLPIVSLLPGFGIFSNNKN
LEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSAISSNKYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIKRN
VKEVLGYPVIVKPAVLGSSIGINVAYSENQIESFIKEALKYDLTIVIEKFIEAREIECSIIGNEKMKIFSPGEVVVQDFI
FYDYDAKYSVIPGNSIIFNIPAHLETNQLLSIKEYAFLTYKNLELRGMARVDFFVEKKSGTIYLNEINTIPGFTDISMFA
KMCSNDGLQFKDLVDNLIDYAFQSYINRKKRIDFEN
>Mature_356_residues
MLIFGGVSFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINTDVLPIVSLLPGFGIFSNNKN
LEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSAISSNKYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIKRN
VKEVLGYPVIVKPAVLGSSIGINVAYSENQIESFIKEALKYDLTIVIEKFIEAREIECSIIGNEKMKIFSPGEVVVQDFI
FYDYDAKYSVIPGNSIIFNIPAHLETNQLLSIKEYAFLTYKNLELRGMARVDFFVEKKSGTIYLNEINTIPGFTDISMFA
KMCSNDGLQFKDLVDNLIDYAFQSYINRKKRIDFEN

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=34.453781512605, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1786280, Length=342, Percent_Identity=28.9473684210526, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40223; Mature: 40223

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFGGVSFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINT
CEEECCCCCCCCCCCCHHEEEEEEEEECCCEEEEEEEEECCCCEEEEEECCCCCCCCCCC
DVLPIVSLLPGFGIFSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSA
CHHHHHHHCCCCCEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHH
ISSNKYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIKRNVKEVLGYPVIVKPAVLGSSI
HCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCC
GINVAYSENQIESFIKEALKYDLTIVIEKFIEAREIECSIIGNEKMKIFSPGEVVVQDFI
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCHHHEEEHE
FYDYDAKYSVIPGNSIIFNIPAHLETNQLLSIKEYAFLTYKNLELRGMARVDFFVEKKSG
EEECCCEEEEECCCEEEEEECCCCCCCCEEEEEHEEEEEEECCEEEEEEEEEEEEEECCC
TIYLNEINTIPGFTDISMFAKMCSNDGLQFKDLVDNLIDYAFQSYINRKKRIDFEN
EEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLIFGGVSFEHEISCKSAYSIYLALLDLNKYNIYPVYIDKCTGVWYLLDSVSDPPKPINT
CEEECCCCCCCCCCCCHHEEEEEEEEECCCEEEEEEEEECCCCEEEEEECCCCCCCCCCC
DVLPIVSLLPGFGIFSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIGSA
CHHHHHHHCCCCCEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHH
ISSNKYFCKLLLKSFDIPLVPFIGFRQHDYFLDKEEIKRNVKEVLGYPVIVKPAVLGSSI
HCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHCCCCC
GINVAYSENQIESFIKEALKYDLTIVIEKFIEAREIECSIIGNEKMKIFSPGEVVVQDFI
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCHHHEEEHE
FYDYDAKYSVIPGNSIIFNIPAHLETNQLLSIKEYAFLTYKNLELRGMARVDFFVEKKSG
EEECCCEEEEECCCEEEEEECCCCCCCCEEEEEHEEEEEEECCEEEEEEEEEEEEEECCC
TIYLNEINTIPGFTDISMFAKMCSNDGLQFKDLVDNLIDYAFQSYINRKKRIDFEN
EEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA