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The map label for this gene is gap

Identifier: 15594403

GI number: 15594403

Start: 52454

End: 53461

Strand: Reverse

Name: gap

Synonym: BB0057

Alternate gene names: 15594403

Gene position: 53461-52454 (Counterclockwise)

Preceding gene: 15594404

Following gene: 15594402

Centisome position: 5.87

GC content: 36.11

Gene sequence:

>1008_bases
ATGAAATTGGCTATTAATGGCTTTGGGCGTATTGGTAGAAATGTTTTTAAGATTGCTTTTGAAAGAGGAATTGATATTGT
CGCAATAAATGATTTAACAGATCCTAAAACTCTTGCACATCTTTTAAAGTATGATTCGACTTTTGGAGTATATAATAAAA
AAGTTGAGTCAAGAGATGGTGCCATTGTTGTGGATGGAAGAGAAATAAAAATTATTGCTGAGAGAGACCCAAAAAATCTT
CCTTGGGCAAAGCTTGGAATTGATGTTGTAATTGAGTCAACAGGTGTTTTTTCATCAGCAACTAGTGATAAAGGGGGATA
TCTTGATCATGTGAATCATGCTGGGGCTAAGAAAGTTATTTTAACAGTTCCTGCTAAAGACGAGATTAAAACAATAGTTC
TTGGTGTAAATGATCACGATATTAATTCTGATTTAAAGGCTGTGTCAAATGCTTCATGTACAACCAATTGCTTGGCTCCT
CTTGCAAAAGTACTTCATGAATCATTTGGAATTGAACAAGGACTTATGACAACAGTTCATGCTTATACTAATGATCAAAG
AATTTTGGATCTTCCTCATTCTGATCTTAGACGGGCAAGAGCTGCTGCACTTTCTATTATTCCAACTTCAACCGGTGCTG
CAAAAGCAGTTGGACTTGTTTTGCCCGAACTTAAGGGTAAGTTGAATGGTACTTCAATGAGGGTGCCTGTGCCAACAGGT
TCAATAGTTGACCTTACAGTTCAACTTAAGAAAAAGGATGTTACAAAAGAAGAGATTAATTCTGTGCTCAGGAAGGCATC
AGAAACGCCTGAGCTTAAAGGTATTTTGGGGTACACGGAAGATCCAATTGTATCTTCAGATATAAAGGGGAATTCTCATT
CTTCTATTGTTGATGGTCTTGAGACAATGGTTCTTGAAAATGGATTTGCAAAGATATTGTCATGGTATGACAATGAGTTT
GGATATTCTACAAGAGTGGTTGATCTTGCTCAAAAATTGGTAAAATAA

Upstream 100 bases:

>100_bases
AGGAGCCACTGTGCTTATAAAAAGATAGTTTGACACTTGTTGTTAATTTGTGATTTAATAATTCTAGTAAGAATAAAATC
AAAAAATTGGAGGATTTTGT

Downstream 100 bases:

>100_bases
GTAAGCTTAAGGTAATAAAATGTCAATAAAAACAGTAAAAGACTTTAGTAGCTTTGCGGGCAAGCGAGCTTTAGTAAGAT
GTGATTTTAATGTTCCCCTA

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKLAINGFGRIGRNVFKIAFERGIDIVAINDLTDPKTLAHLLKYDSTFGVYNKKVESRDGAIVVDGREIKIIAERDPKNL
PWAKLGIDVVIESTGVFSSATSDKGGYLDHVNHAGAKKVILTVPAKDEIKTIVLGVNDHDINSDLKAVSNASCTTNCLAP
LAKVLHESFGIEQGLMTTVHAYTNDQRILDLPHSDLRRARAAALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
SIVDLTVQLKKKDVTKEEINSVLRKASETPELKGILGYTEDPIVSSDIKGNSHSSIVDGLETMVLENGFAKILSWYDNEF
GYSTRVVDLAQKLVK

Sequences:

>Translated_335_residues
MKLAINGFGRIGRNVFKIAFERGIDIVAINDLTDPKTLAHLLKYDSTFGVYNKKVESRDGAIVVDGREIKIIAERDPKNL
PWAKLGIDVVIESTGVFSSATSDKGGYLDHVNHAGAKKVILTVPAKDEIKTIVLGVNDHDINSDLKAVSNASCTTNCLAP
LAKVLHESFGIEQGLMTTVHAYTNDQRILDLPHSDLRRARAAALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
SIVDLTVQLKKKDVTKEEINSVLRKASETPELKGILGYTEDPIVSSDIKGNSHSSIVDGLETMVLENGFAKILSWYDNEF
GYSTRVVDLAQKLVK
>Mature_335_residues
MKLAINGFGRIGRNVFKIAFERGIDIVAINDLTDPKTLAHLLKYDSTFGVYNKKVESRDGAIVVDGREIKIIAERDPKNL
PWAKLGIDVVIESTGVFSSATSDKGGYLDHVNHAGAKKVILTVPAKDEIKTIVLGVNDHDINSDLKAVSNASCTTNCLAP
LAKVLHESFGIEQGLMTTVHAYTNDQRILDLPHSDLRRARAAALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
SIVDLTVQLKKKDVTKEEINSVLRKASETPELKGILGYTEDPIVSSDIKGNSHSSIVDGLETMVLENGFAKILSWYDNEF
GYSTRVVDLAQKLVK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7657116, Length=336, Percent_Identity=48.5119047619048, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI7669492, Length=336, Percent_Identity=50.297619047619, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1788079, Length=337, Percent_Identity=51.9287833827893, Blast_Score=330, Evalue=6e-92,
Organism=Escherichia coli, GI1789295, Length=333, Percent_Identity=41.1411411411411, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17534677, Length=338, Percent_Identity=48.2248520710059, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17534679, Length=338, Percent_Identity=47.9289940828402, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=46.4285714285714, Blast_Score=270, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=46.4285714285714, Blast_Score=270, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6322468, Length=338, Percent_Identity=53.5502958579882, Blast_Score=336, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6321631, Length=338, Percent_Identity=52.9585798816568, Blast_Score=333, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6322409, Length=338, Percent_Identity=51.1834319526627, Blast_Score=323, Evalue=2e-89,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=50, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=50, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=49.4011976047904, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=49.4011976047904, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI19922412, Length=330, Percent_Identity=47.5757575757576, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_BORBU (P46795)

Other databases:

- EMBL:   U28760
- EMBL:   AE000783
- PIR:   A70107
- RefSeq:   NP_212191.1
- PDB:   3HJA
- PDBsum:   3HJA
- ProteinModelPortal:   P46795
- EnsemblBacteria:   EBBORT00000008599
- GeneID:   1194893
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0057
- NMPDR:   fig|224326.1.peg.441
- TIGR:   BB_0057
- GeneTree:   EBGT00050000007066
- HOGENOM:   HBG571736
- OMA:   EHEITSD
- PhylomeDB:   P46795
- ProtClustDB:   CLSK507322
- BioCyc:   BBUR224326:BB_0057-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 36255; Mature: 36255

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 153-153 BINDING 31-31 BINDING 75-75 BINDING 183-183 BINDING 198-198 BINDING 234-234 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAINGFGRIGRNVFKIAFERGIDIVAINDLTDPKTLAHLLKYDSTFGVYNKKVESRDG
CEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHCEECCCCC
AIVVDGREIKIIAERDPKNLPWAKLGIDVVIESTGVFSSATSDKGGYLDHVNHAGAKKVI
EEEECCCEEEEEECCCCCCCCHHHCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCEEE
LTVPAKDEIKTIVLGVNDHDINSDLKAVSNASCTTNCLAPLAKVLHESFGIEQGLMTTVH
EECCCCCCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEE
AYTNDQRILDLPHSDLRRARAAALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
EECCCCEEEECCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCC
SIVDLTVQLKKKDVTKEEINSVLRKASETPELKGILGYTEDPIVSSDIKGNSHSSIVDGL
CEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHH
ETMVLENGFAKILSWYDNEFGYSTRVVDLAQKLVK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAINGFGRIGRNVFKIAFERGIDIVAINDLTDPKTLAHLLKYDSTFGVYNKKVESRDG
CEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHCEECCCCC
AIVVDGREIKIIAERDPKNLPWAKLGIDVVIESTGVFSSATSDKGGYLDHVNHAGAKKVI
EEEECCCEEEEEECCCCCCCCHHHCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCEEE
LTVPAKDEIKTIVLGVNDHDINSDLKAVSNASCTTNCLAPLAKVLHESFGIEQGLMTTVH
EECCCCCCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEE
AYTNDQRILDLPHSDLRRARAAALSIIPTSTGAAKAVGLVLPELKGKLNGTSMRVPVPTG
EECCCCEEEECCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCC
SIVDLTVQLKKKDVTKEEINSVLRKASETPELKGILGYTEDPIVSSDIKGNSHSSIVDGL
CEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHH
ETMVLENGFAKILSWYDNEFGYSTRVVDLAQKLVK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8557349; 9403685