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The map label for this gene is pgk [H]

Identifier: 15594402

GI number: 15594402

Start: 51253

End: 52434

Strand: Reverse

Name: pgk [H]

Synonym: BB0056

Alternate gene names: 15594402

Gene position: 52434-51253 (Counterclockwise)

Preceding gene: 15594403

Following gene: 15594401

Centisome position: 5.76

GC content: 35.7

Gene sequence:

>1182_bases
ATGTCAATAAAAACAGTAAAAGACTTTAGTAGCTTTGCGGGCAAGCGAGCTTTAGTAAGATGTGATTTTAATGTTCCCCT
AAAAGAGGGGAGCATTAGCGATGATACTAGAATTAGGGCAGCATTGTCTACAATAGAGTATCTAAAAGAAAGAGGAGCTA
GGATTGTGCTTGTAAGTCATTTGGGTAGACCAGATGGAAAGAAAAATCCTAAATATTCTCTTAAGCCGGTTGCCAATAGG
TTGTCAGAGCTTTTAGGCCAAGATGTCAAGATGCTTTCTGATTGTATTGGAAGTGAGGTTGTAAATAGTACCTTGCAAAT
GAAGGATGGGGATGTTGTATTGCTTGAAAATGTAAGGTTTTATGCAGAAGAAGAGAAAAACGATAAAAATTTTGCAAAAA
AACTTTCTGAAAATGGAGATGTTTTTGTAAATGATGCTTTTGGCGCAGCTCATAGAGCTCATGCTTCAACTGTGGGAGTT
GCTGATTATTTGCCGTCTGTTGGTGGATTTTTAATGGAAAAAGAAGATAAATTTCTTGGTGGAATATTAAAAAATCCCGA
AAGGCCATTTGTTTCAATTATTGGTGGTTCTAAGGTTTCTTCAAAGATTGCGGTACTAGAATCACTTTTGTCAAAGTCTA
ATGTAGTTGTGATTGGCGGTGGAATGGCTTATACTTTTTTGCATTCCGAAGGATATTCTATAGGCAAATCTCTTTTAGAG
GACGAATATATTGGTATAGCATCTTCTTTTTTAAAGAAAGCAAAAGAGCTGGGCGTTAAAGTAATTTTACCACTTGATCA
TATTGTAGCTGATGATTTTAACAAAAATTCAATTCCTGAGTATATTGACTCCTTTAATATTCCTGAAAACAAGATTGGCA
TGGATATTGGAGCTAATACTTTAAAAGAAATTGAAAATGTTGTGAAAACTGCCAAAACTATAATTTGGAATGGTCCTCTT
GGTGTATTTGAATTTGATTCATTTTCAAAAGGGACTGCTAAGGTTGCCGAAATGGTAGCATCTTGCTCAGGTTTAACCGT
TGTTGGTGGTGGAGATTCTGTTGCAGCTGTTAATAAATTTAATTTATCTGATAAGATTACTCATGTTTCAACGGGCGGTG
GTGCATCGCTTGAATATCTTGAAGGGAGAATTTTGCCGGGTATTAAGGTTTTGGAGAATTAA

Upstream 100 bases:

>100_bases
GCAAAGATATTGTCATGGTATGACAATGAGTTTGGATATTCTACAAGAGTGGTTGATCTTGCTCAAAAATTGGTAAAATA
AGTAAGCTTAAGGTAATAAA

Downstream 100 bases:

>100_bases
AATGAGAAAAACATTTTTAGCGGGAAATTGGAAAATGCATTATACAAGTGCAGAAGCTTCTATTGTTGCAAAAAAAATTG
CAACTGAGGTTAAGACCTTA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSIKTVKDFSSFAGKRALVRCDFNVPLKEGSISDDTRIRAALSTIEYLKERGARIVLVSHLGRPDGKKNPKYSLKPVANR
LSELLGQDVKMLSDCIGSEVVNSTLQMKDGDVVLLENVRFYAEEEKNDKNFAKKLSENGDVFVNDAFGAAHRAHASTVGV
ADYLPSVGGFLMEKEDKFLGGILKNPERPFVSIIGGSKVSSKIAVLESLLSKSNVVVIGGGMAYTFLHSEGYSIGKSLLE
DEYIGIASSFLKKAKELGVKVILPLDHIVADDFNKNSIPEYIDSFNIPENKIGMDIGANTLKEIENVVKTAKTIIWNGPL
GVFEFDSFSKGTAKVAEMVASCSGLTVVGGGDSVAAVNKFNLSDKITHVSTGGGASLEYLEGRILPGIKVLEN

Sequences:

>Translated_393_residues
MSIKTVKDFSSFAGKRALVRCDFNVPLKEGSISDDTRIRAALSTIEYLKERGARIVLVSHLGRPDGKKNPKYSLKPVANR
LSELLGQDVKMLSDCIGSEVVNSTLQMKDGDVVLLENVRFYAEEEKNDKNFAKKLSENGDVFVNDAFGAAHRAHASTVGV
ADYLPSVGGFLMEKEDKFLGGILKNPERPFVSIIGGSKVSSKIAVLESLLSKSNVVVIGGGMAYTFLHSEGYSIGKSLLE
DEYIGIASSFLKKAKELGVKVILPLDHIVADDFNKNSIPEYIDSFNIPENKIGMDIGANTLKEIENVVKTAKTIIWNGPL
GVFEFDSFSKGTAKVAEMVASCSGLTVVGGGDSVAAVNKFNLSDKITHVSTGGGASLEYLEGRILPGIKVLEN
>Mature_392_residues
SIKTVKDFSSFAGKRALVRCDFNVPLKEGSISDDTRIRAALSTIEYLKERGARIVLVSHLGRPDGKKNPKYSLKPVANRL
SELLGQDVKMLSDCIGSEVVNSTLQMKDGDVVLLENVRFYAEEEKNDKNFAKKLSENGDVFVNDAFGAAHRAHASTVGVA
DYLPSVGGFLMEKEDKFLGGILKNPERPFVSIIGGSKVSSKIAVLESLLSKSNVVVIGGGMAYTFLHSEGYSIGKSLLED
EYIGIASSFLKKAKELGVKVILPLDHIVADDFNKNSIPEYIDSFNIPENKIGMDIGANTLKEIENVVKTAKTIIWNGPLG
VFEFDSFSKGTAKVAEMVASCSGLTVVGGGDSVAAVNKFNLSDKITHVSTGGGASLEYLEGRILPGIKVLEN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=416, Percent_Identity=44.7115384615385, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=45.4326923076923, Blast_Score=343, Evalue=2e-94,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=41.7721518987342, Blast_Score=311, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17508823, Length=405, Percent_Identity=46.1728395061728, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319857, Length=397, Percent_Identity=46.095717884131, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI17136394, Length=401, Percent_Identity=45.3865336658354, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24581203, Length=403, Percent_Identity=43.424317617866, Blast_Score=330, Evalue=9e-91,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42346; Mature: 42215

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKTVKDFSSFAGKRALVRCDFNVPLKEGSISDDTRIRAALSTIEYLKERGARIVLVSH
CCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
LGRPDGKKNPKYSLKPVANRLSELLGQDVKMLSDCIGSEVVNSTLQMKDGDVVLLENVRF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECEEE
YAEEEKNDKNFAKKLSENGDVFVNDAFGAAHRAHASTVGVADYLPSVGGFLMEKEDKFLG
EHHHCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHH
GILKNPERPFVSIIGGSKVSSKIAVLESLLSKSNVVVIGGGMAYTFLHSEGYSIGKSLLE
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCHHHHHHHHH
DEYIGIASSFLKKAKELGVKVILPLDHIVADDFNKNSIPEYIDSFNIPENKIGMDIGANT
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCCCHHHHHCCCCCCHHCCCCCCHHH
LKEIENVVKTAKTIIWNGPLGVFEFDSFSKGTAKVAEMVASCSGLTVVGGGDSVAAVNKF
HHHHHHHHHHHHHEEECCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEEEEEE
NLSDKITHVSTGGGASLEYLEGRILPGIKVLEN
CCCCCEEEEECCCCCCHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
SIKTVKDFSSFAGKRALVRCDFNVPLKEGSISDDTRIRAALSTIEYLKERGARIVLVSH
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
LGRPDGKKNPKYSLKPVANRLSELLGQDVKMLSDCIGSEVVNSTLQMKDGDVVLLENVRF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECEEE
YAEEEKNDKNFAKKLSENGDVFVNDAFGAAHRAHASTVGVADYLPSVGGFLMEKEDKFLG
EHHHCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHH
GILKNPERPFVSIIGGSKVSSKIAVLESLLSKSNVVVIGGGMAYTFLHSEGYSIGKSLLE
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCHHHHHHHHH
DEYIGIASSFLKKAKELGVKVILPLDHIVADDFNKNSIPEYIDSFNIPENKIGMDIGANT
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCCCHHHHHCCCCCCHHCCCCCCHHH
LKEIENVVKTAKTIIWNGPLGVFEFDSFSKGTAKVAEMVASCSGLTVVGGGDSVAAVNKF
HHHHHHHHHHHHHEEECCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEEEEEE
NLSDKITHVSTGGGASLEYLEGRILPGIKVLEN
CCCCCEEEEECCCCCCHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA