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The map label for this gene is ruvB [H]

Identifier: 15594368

GI number: 15594368

Start: 21058

End: 22101

Strand: Reverse

Name: ruvB [H]

Synonym: BB0022

Alternate gene names: 15594368

Gene position: 22101-21058 (Counterclockwise)

Preceding gene: 15594369

Following gene: 15594367

Centisome position: 2.43

GC content: 34.29

Gene sequence:

>1044_bases
ATGAAAGACGAAAATAGTATAAGCTTTTTAAGCTCTAATGAAAATTATTTATATGATAAGAGTGAAAATGAGCTTAGGCC
TAAAGTTTTTGAAGATTTTAAAGGTCAGGTTAATGTTAAAGAAACCCTTAGTATTTTCATAAGAGCTTCTAAAGAGAGAG
ATGAGGCTCTAGATCATGTGTTTTTAAGTGGCCCACCGGGTCTTGGGAAAACCACTCTTGCAAGTATTATTGCCTTTGAG
ATGAATGCTTCTATTAAGATTACTTCAGCTCCGGCTTTTGACAAACCAAAAGATATTATTGGAATTTTAACAGGTCTTGA
TGAGAAGAGTGTTTTATTTATTGATGAAATACATAGGCTCAGACCAATAATAGAAGAAATGCTTTGCATTGCCATGGAAG
ATTATGAGCTGGATTGGGTAATAGGGCAAGGAGCCAATGCAAGAACTGTTCGAATGCCACTTCCAAAATTCACATTGATT
GGAGCTACTACTAAACCAGGAAAAGTAACATCTCCACTTTATGCGAGATTTGGAATTACTGCAAGATTTGAACTTTACAG
TGAAATAGAGCTTGTTGAGATAATAAAGAGAAATTCTCTTATTTTAAATATTGAAATAGAAGAGGATGCTGCTTTTCTTC
TTGCAAGAAGCTCAAGAGGGACTCCTCGTATAGCAAATAGACTTTTAAGACGAATAAGAGATATTGCTCAGGTAACTGGA
AGTTTGGTTATTACAAGTGATATTGTTTCAATTGGGCTTGAAATGCTTAGAATTGATGGGGAAGGCCTTGATGAGCAAGA
TAGAAATATTTTAAGAAGTTTAATATTGAAATTTAATGGAGGACCTGTAGGTGTTGATACTTTAGCTATTTCTGTAGGAG
AAACAGCAGATTCTCTTGAAGACTTTTATGAGCCTTATTTAATTATGAAAGGATTTATCAGTAGAACTCACAGAGGTCGT
AAAGCTACTGAGTTTGCATACCTTCACTTAAACTTAGAGATGAAAGAGGATGGTCTTAATGAAAACCAAAGAGTTTCATT
TTAA

Upstream 100 bases:

>100_bases
ATTCTGAAAAGGAGCAGTTTTTATTTAAGGAGGTTTTAAAAAGAATATCGAATTAATTATTAGTTTTTTATAGTTATAAA
ATAGTTTTTAAGGAATATTT

Downstream 100 bases:

>100_bases
TTTATCCCATTCTTTAATAGAGCAATATCCAAGTGAAAAAAGAGGATCTTCAAGGTTAATCGTGCTAGATCCTCAATTGC
AAAAAATTTACCATAAAAAT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKDENSISFLSSNENYLYDKSENELRPKVFEDFKGQVNVKETLSIFIRASKERDEALDHVFLSGPPGLGKTTLASIIAFE
MNASIKITSAPAFDKPKDIIGILTGLDEKSVLFIDEIHRLRPIIEEMLCIAMEDYELDWVIGQGANARTVRMPLPKFTLI
GATTKPGKVTSPLYARFGITARFELYSEIELVEIIKRNSLILNIEIEEDAAFLLARSSRGTPRIANRLLRRIRDIAQVTG
SLVITSDIVSIGLEMLRIDGEGLDEQDRNILRSLILKFNGGPVGVDTLAISVGETADSLEDFYEPYLIMKGFISRTHRGR
KATEFAYLHLNLEMKEDGLNENQRVSF

Sequences:

>Translated_347_residues
MKDENSISFLSSNENYLYDKSENELRPKVFEDFKGQVNVKETLSIFIRASKERDEALDHVFLSGPPGLGKTTLASIIAFE
MNASIKITSAPAFDKPKDIIGILTGLDEKSVLFIDEIHRLRPIIEEMLCIAMEDYELDWVIGQGANARTVRMPLPKFTLI
GATTKPGKVTSPLYARFGITARFELYSEIELVEIIKRNSLILNIEIEEDAAFLLARSSRGTPRIANRLLRRIRDIAQVTG
SLVITSDIVSIGLEMLRIDGEGLDEQDRNILRSLILKFNGGPVGVDTLAISVGETADSLEDFYEPYLIMKGFISRTHRGR
KATEFAYLHLNLEMKEDGLNENQRVSF
>Mature_347_residues
MKDENSISFLSSNENYLYDKSENELRPKVFEDFKGQVNVKETLSIFIRASKERDEALDHVFLSGPPGLGKTTLASIIAFE
MNASIKITSAPAFDKPKDIIGILTGLDEKSVLFIDEIHRLRPIIEEMLCIAMEDYELDWVIGQGANARTVRMPLPKFTLI
GATTKPGKVTSPLYARFGITARFELYSEIELVEIIKRNSLILNIEIEEDAAFLLARSSRGTPRIANRLLRRIRDIAQVTG
SLVITSDIVSIGLEMLRIDGEGLDEQDRNILRSLILKFNGGPVGVDTLAISVGETADSLEDFYEPYLIMKGFISRTHRGR
KATEFAYLHLNLEMKEDGLNENQRVSF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=328, Percent_Identity=48.780487804878, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 39006; Mature: 39006

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00690 DEAH_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDENSISFLSSNENYLYDKSENELRPKVFEDFKGQVNVKETLSIFIRASKERDEALDHV
CCCCCCEEEEECCCCEEEECCCCCCCCHHHHHCCCCCCHHHHEEEEEEECCCHHHHHHEE
FLSGPPGLGKTTLASIIAFEMNASIKITSAPAFDKPKDIIGILTGLDEKSVLFIDEIHRL
EECCCCCCCHHHHHHHHHEEECCEEEEECCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHH
RPIIEEMLCIAMEDYELDWVIGQGANARTVRMPLPKFTLIGATTKPGKVTSPLYARFGIT
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHCCE
ARFELYSEIELVEIIKRNSLILNIEIEEDAAFLLARSSRGTPRIANRLLRRIRDIAQVTG
EEEHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
SLVITSDIVSIGLEMLRIDGEGLDEQDRNILRSLILKFNGGPVGVDTLAISVGETADSLE
CEEEEHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHEECCCCCCCEEEEEEECCCHHHHH
DFYEPYLIMKGFISRTHRGRKATEFAYLHLNLEMKEDGLNENQRVSF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKDENSISFLSSNENYLYDKSENELRPKVFEDFKGQVNVKETLSIFIRASKERDEALDHV
CCCCCCEEEEECCCCEEEECCCCCCCCHHHHHCCCCCCHHHHEEEEEEECCCHHHHHHEE
FLSGPPGLGKTTLASIIAFEMNASIKITSAPAFDKPKDIIGILTGLDEKSVLFIDEIHRL
EECCCCCCCHHHHHHHHHEEECCEEEEECCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHH
RPIIEEMLCIAMEDYELDWVIGQGANARTVRMPLPKFTLIGATTKPGKVTSPLYARFGIT
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHCCE
ARFELYSEIELVEIIKRNSLILNIEIEEDAAFLLARSSRGTPRIANRLLRRIRDIAQVTG
EEEHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
SLVITSDIVSIGLEMLRIDGEGLDEQDRNILRSLILKFNGGPVGVDTLAISVGETADSLE
CEEEEHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHEECCCCCCCEEEEEEECCCHHHHH
DFYEPYLIMKGFISRTHRGRKATEFAYLHLNLEMKEDGLNENQRVSF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA