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The map label for this gene is queA [H]

Identifier: 15594367

GI number: 15594367

Start: 20445

End: 21092

Strand: Reverse

Name: queA [H]

Synonym: BB0021

Alternate gene names: 15594367

Gene position: 21092-20445 (Counterclockwise)

Preceding gene: 15594368

Following gene: 15594366

Centisome position: 2.32

GC content: 28.24

Gene sequence:

>648_bases
ATGGTCTTAATGAAAACCAAAGAGTTTCATTTTAATTTATCCCATTCTTTAATAGAGCAATATCCAAGTGAAAAAAGAGG
ATCTTCAAGGTTAATCGTGCTAGATCCTCAATTGCAAAAAATTTACCATAAAAATTCTGTAAACAATATTTTAAAATATA
TAAATAGTAATATTTTTAATAACTCAAAAGCTAGAAAATCAAGAATATATGCAGAATCAGAGATGAGCAGTAATGTTGAA
TTTTTAATTTTGGATAGAATTGACACTAATTTATTCACTGCGTTAGTTTCTAAGTCAAAAAAGCAAATTATTGGCAATTT
TTACAAATTCCCCGAAGGATTAATGGATGAAATTTTGTCAAAAAATAGTAGTGAGGTTGTTTTAAAATTTAACAATAATG
TTGGTGAAGATTATTTTGAAAAACATTGTTTTGTGCCTTTGCCCTCTTACATTAAAAGAGATTATGATAAGATAGATGAA
GATCGATATCAAACTATTTATTCTAAATATGTTGGATCTACAGCTTCTGCAACTGCAGGGTTACATTTTAGCAGAGATTT
GTTTTCTGCCTTTGAAAATAACAATATTGAATATGATTTTATCACTCTTCATGTGGGGGCTTGGGACTTTTCTTCCTGTA
AGATCTAA

Upstream 100 bases:

>100_bases
GAGCCTTATTTAATTATGAAAGGATTTATCAGTAGAACTCACAGAGGTCGTAAAGCTACTGAGTTTGCATACCTTCACTT
AAACTTAGAGATGAAAGAGG

Downstream 100 bases:

>100_bases
AAAGGTTGAAGAACATAATATGCATTTTGAAACTTTTTTAATAAAAGATTTTGTGGCTGTGAGATTGCAGAATGCAAAAC
TTCTTGGTAAAAGAATTTTG

Product: S-adenosylmethionine: tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MVLMKTKEFHFNLSHSLIEQYPSEKRGSSRLIVLDPQLQKIYHKNSVNNILKYINSNIFNNSKARKSRIYAESEMSSNVE
FLILDRIDTNLFTALVSKSKKQIIGNFYKFPEGLMDEILSKNSSEVVLKFNNNVGEDYFEKHCFVPLPSYIKRDYDKIDE
DRYQTIYSKYVGSTASATAGLHFSRDLFSAFENNNIEYDFITLHVGAWDFSSCKI

Sequences:

>Translated_215_residues
MVLMKTKEFHFNLSHSLIEQYPSEKRGSSRLIVLDPQLQKIYHKNSVNNILKYINSNIFNNSKARKSRIYAESEMSSNVE
FLILDRIDTNLFTALVSKSKKQIIGNFYKFPEGLMDEILSKNSSEVVLKFNNNVGEDYFEKHCFVPLPSYIKRDYDKIDE
DRYQTIYSKYVGSTASATAGLHFSRDLFSAFENNNIEYDFITLHVGAWDFSSCKI
>Mature_215_residues
MVLMKTKEFHFNLSHSLIEQYPSEKRGSSRLIVLDPQLQKIYHKNSVNNILKYINSNIFNNSKARKSRIYAESEMSSNVE
FLILDRIDTNLFTALVSKSKKQIIGNFYKFPEGLMDEILSKNSSEVVLKFNNNVGEDYFEKHCFVPLPSYIKRDYDKIDE
DRYQTIYSKYVGSTASATAGLHFSRDLFSAFENNNIEYDFITLHVGAWDFSSCKI

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=218, Percent_Identity=28.8990825688073, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 24995; Mature: 24995

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLMKTKEFHFNLSHSLIEQYPSEKRGSSRLIVLDPQLQKIYHKNSVNNILKYINSNIFN
CEEEECCHHEECHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCC
NSKARKSRIYAESEMSSNVEFLILDRIDTNLFTALVSKSKKQIIGNFYKFPEGLMDEILS
CCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
KNSSEVVLKFNNNVGEDYFEKHCFVPLPSYIKRDYDKIDEDRYQTIYSKYVGSTASATAG
CCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
LHFSRDLFSAFENNNIEYDFITLHVGAWDFSSCKI
CHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MVLMKTKEFHFNLSHSLIEQYPSEKRGSSRLIVLDPQLQKIYHKNSVNNILKYINSNIFN
CEEEECCHHEECHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCC
NSKARKSRIYAESEMSSNVEFLILDRIDTNLFTALVSKSKKQIIGNFYKFPEGLMDEILS
CCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
KNSSEVVLKFNNNVGEDYFEKHCFVPLPSYIKRDYDKIDEDRYQTIYSKYVGSTASATAG
CCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
LHFSRDLFSAFENNNIEYDFITLHVGAWDFSSCKI
CHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]