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The map label for this gene is ruvA

Identifier: 15594369

GI number: 15594369

Start: 22146

End: 22739

Strand: Reverse

Name: ruvA

Synonym: BB0023

Alternate gene names: 15594369

Gene position: 22739-22146 (Counterclockwise)

Preceding gene: 15594371

Following gene: 15594368

Centisome position: 2.5

GC content: 29.29

Gene sequence:

>594_bases
ATGATAAATAAGATTCATGGTAAAGTTATAGAAAAAAAAGAATCTAGTTTAGTTTTAATGACTACTGTTTTTGAATTTGA
ACTTTTAGTTAGTGCATTTTGCCTTGCTAATTTTAATTTGTCAGACAAAGTTGAGTTATTTACTTATCTTTATACTAGAG
AAAATGAATTAAAACTTTTTGGATTTTTGAATTCAGATGAAAGAGAGATCTTTAAGGAGCTTATTGGAGTAAGCGGAGTA
GGCCCAAGGGCCGCTTTAAGAGTGTTATCCAATATAAGGTATAATGAGTTTAAGGAGGCTATTGACAAAGAGGATATTGA
ACTTGTCTCTAAAATCAAAGGCATTGGCAAAAAAATGGCTGGTAAAATGTTTTTACATCTTCAAGGTAAGCTTTTGATTA
ATAGCGAGCTTGAATCCACTGGTCTTTTTAGATTTAAAGAATTAGAAGAATCAATTGTTAGTATGGGATTTGATAGAAAA
ATTGTTAATAGCAAGATTAGAGAGGCTTTCAATTTAGCTGAATTTGCAAATTTAAAAGATTCTGAAAAGGAGCAGTTTTT
ATTTAAGGAGGTTTTAAAAAGAATATCGAATTAA

Upstream 100 bases:

>100_bases
ATTTAAAAATTGATTTTTCATTTAGTGTCCTTTTTTTACTTAAGGCTTTTGCTTTAAATTGTTGAATGTTTCTGTGTAAT
ATAATTTATAATTTAATTAT

Downstream 100 bases:

>100_bases
TTATTAGTTTTTTATAGTTATAAAATAGTTTTTAAGGAATATTTATGAAAGACGAAAATAGTATAAGCTTTTTAAGCTCT
AATGAAAATTATTTATATGA

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MINKIHGKVIEKKESSLVLMTTVFEFELLVSAFCLANFNLSDKVELFTYLYTRENELKLFGFLNSDEREIFKELIGVSGV
GPRAALRVLSNIRYNEFKEAIDKEDIELVSKIKGIGKKMAGKMFLHLQGKLLINSELESTGLFRFKELEESIVSMGFDRK
IVNSKIREAFNLAEFANLKDSEKEQFLFKEVLKRISN

Sequences:

>Translated_197_residues
MINKIHGKVIEKKESSLVLMTTVFEFELLVSAFCLANFNLSDKVELFTYLYTRENELKLFGFLNSDEREIFKELIGVSGV
GPRAALRVLSNIRYNEFKEAIDKEDIELVSKIKGIGKKMAGKMFLHLQGKLLINSELESTGLFRFKELEESIVSMGFDRK
IVNSKIREAFNLAEFANLKDSEKEQFLFKEVLKRISN
>Mature_197_residues
MINKIHGKVIEKKESSLVLMTTVFEFELLVSAFCLANFNLSDKVELFTYLYTRENELKLFGFLNSDEREIFKELIGVSGV
GPRAALRVLSNIRYNEFKEAIDKEDIELVSKIKGIGKKMAGKMFLHLQGKLLINSELESTGLFRFKELEESIVSMGFDRK
IVNSKIREAFNLAEFANLKDSEKEQFLFKEVLKRISN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=133, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_BORBU (P70827)

Other databases:

- EMBL:   Y08885
- EMBL:   AE000783
- PIR:   G70102
- RefSeq:   NP_212157.1
- ProteinModelPortal:   P70827
- SMR:   P70827
- EnsemblBacteria:   EBBORT00000008922
- GeneID:   1194859
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0023
- NMPDR:   fig|224326.1.peg.407
- TIGR:   BB_0023
- GeneTree:   EBGT00050000007395
- HOGENOM:   HBG635309
- OMA:   SEREVFE
- ProtClustDB:   PRK13901
- BioCyc:   BBUR224326:BB_0023-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 22689; Mature: 22689

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKIHGKVIEKKESSLVLMTTVFEFELLVSAFCLANFNLSDKVELFTYLYTRENELKLF
CCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEE
GFLNSDEREIFKELIGVSGVGPRAALRVLSNIRYNEFKEAIDKEDIELVSKIKGIGKKMA
EECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
GKMFLHLQGKLLINSELESTGLFRFKELEESIVSMGFDRKIVNSKIREAFNLAEFANLKD
HHHEEEEECEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
SEKEQFLFKEVLKRISN
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINKIHGKVIEKKESSLVLMTTVFEFELLVSAFCLANFNLSDKVELFTYLYTRENELKLF
CCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEE
GFLNSDEREIFKELIGVSGVGPRAALRVLSNIRYNEFKEAIDKEDIELVSKIKGIGKKMA
EECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHH
GKMFLHLQGKLLINSELESTGLFRFKELEESIVSMGFDRKIVNSKIREAFNLAEFANLKD
HHHEEEEECEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
SEKEQFLFKEVLKRISN
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9766225; 9403685