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The map label for this gene is leuC

Identifier: 154686962

GI number: 154686962

Start: 2654096

End: 2655514

Strand: Reverse

Name: leuC

Synonym: RBAM_025320

Alternate gene names: 154686962

Gene position: 2655514-2654096 (Counterclockwise)

Preceding gene: 154686963

Following gene: 154686961

Centisome position: 67.77

GC content: 51.02

Gene sequence:

>1419_bases
ATGATGCCTCGAACAATCATCGAAAAGATTTGGGATCGGCATGTTGTAAAAAGCGGTGAAGGAAAACCCGACCTTCTCTA
TATTGATCTGCACCTCATTCATGAGGTGACGTCTCCTCAGGCGTTTGAAGGGTTAAGACAGAAGGGGAGAAAGGTCAGAC
GGCCGCAGAATACATTCGCGACGATGGACCACAATATTCCGACCGTCAACCGTTTTGACATTAAAGATGAAGTCGCAAAG
CGCCAAGTAACCGCGCTTGAACGGAATTGTGAGGAGTTTGGCGTCCGTCTTGCGGATCTTCACAGCGCAGACCAGGGGAT
CGTTCACGTAGTCGGGCCTGAGCTTGGTCTGACACTTCCGGGGAAAACGATCGTTTGCGGAGACAGCCATACATCGACTC
ACGGTGCATTCGGGGCGCTTGCCTTCGGTATCGGCACAAGTGAGGTGGAACATGTTCTTTCCACTCAGACATTGTGGCAG
CAGCGGCCGAAAACGCTTGAAATCCGCGTTGACGGAACGCTCCAAAAAGGAGTGACCGCGAAGGATGTCATCTTGTCGGT
CATCGGAAAATACGGGGTGAAATTCGGTACCGGATATGTCATTGAATACACCGGGGAAGTATTCAGAAACATGTCTATGG
ACGAACGCATGACCGTCTGCAATATGTCGATAGAAGCGGGAGCGCGGGCGGGACTGATCGCTCCGGATGACACGACGATT
GAATATTGTAAAAACCGTAAATACGCGCCAAAAGGCGAGGAATTTGAAAAAGCCGCCGCAGAATGGAAAGCGTTAAAAAC
CGATCCGGGCGCCGTATACGATCATTCCATCGTGTTAGACGGCAGCGAGATTTCTCCAATGGTAACATGGGGCGTCAATC
CCGGCATGGTTCTTCCGGTCGATGCCCAAATACCGGCGCCGGAAACGATTGATTCTGAAGACGGTAAAAAAGAAGCCATC
CGCGCGTACGAATACATGGGAATCTCACCGAATCAAAAAATTGAAGATATTAAAGTCGAGCATGTGTTTATCGGCTCCTG
TACAAATTCGCGGATGACCGATCTTCGCCAGGCTGCGGATATGATCAAAGGCCGAAAAGTCGCTGACAGCGTCAGAGCCA
TTGTGGTCCCGGGTTCTCAAAGCGTGAAACAGCAGGCCGAAGAAGAAGGGCTGCACCGCGTGTTTCTCGAGGCCGGATTT
GAATGGAGAGAATCAGGCTGCAGCATGTGCCTCAGTATGAACAATGATGTCGTGCCTGAAGGGGAACGATGCGCTTCCAC
TTCCAACCGCAACTTCGAAGGCAGACAGGGAAAAGGCGCGAGAACACACCTTGTCAGTCCGGCGATGGCGGCAATGGCAG
CCATTCACGGGCATTTCGTCGATGTAAGAAAGTTTTATCAAGAAAAAACAGTTGTGTAA

Upstream 100 bases:

>100_bases
GAAAAAGTAAAAGCCGCATTAGCCGGCTCGAATACAAAATCTATAGTGTGACGCTTTAAAGAAAGCGGTCTTAGTTCATA
GGTAGAGGGAGGAAACAAAG

Downstream 100 bases:

>100_bases
GGAGTGCGCGAAAAGATGGAACCTTTAAAAACACATACGGGAAAAGCGGCCGTCTTAAACCGGATCAACGTCGATACGGA
CCAGATTATTCCGAAACAGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MMPRTIIEKIWDRHVVKSGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFDIKDEVAK
RQVTALERNCEEFGVRLADLHSADQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QRPKTLEIRVDGTLQKGVTAKDVILSVIGKYGVKFGTGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDDTTI
EYCKNRKYAPKGEEFEKAAAEWKALKTDPGAVYDHSIVLDGSEISPMVTWGVNPGMVLPVDAQIPAPETIDSEDGKKEAI
RAYEYMGISPNQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGRKVADSVRAIVVPGSQSVKQQAEEEGLHRVFLEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV

Sequences:

>Translated_472_residues
MMPRTIIEKIWDRHVVKSGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFDIKDEVAK
RQVTALERNCEEFGVRLADLHSADQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QRPKTLEIRVDGTLQKGVTAKDVILSVIGKYGVKFGTGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDDTTI
EYCKNRKYAPKGEEFEKAAAEWKALKTDPGAVYDHSIVLDGSEISPMVTWGVNPGMVLPVDAQIPAPETIDSEDGKKEAI
RAYEYMGISPNQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGRKVADSVRAIVVPGSQSVKQQAEEEGLHRVFLEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV
>Mature_472_residues
MMPRTIIEKIWDRHVVKSGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFDIKDEVAK
RQVTALERNCEEFGVRLADLHSADQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QRPKTLEIRVDGTLQKGVTAKDVILSVIGKYGVKFGTGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDDTTI
EYCKNRKYAPKGEEFEKAAAEWKALKTDPGAVYDHSIVLDGSEISPMVTWGVNPGMVLPVDAQIPAPETIDSEDGKKEAI
RAYEYMGISPNQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGRKVADSVRAIVVPGSQSVKQQAEEEGLHRVFLEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=28.021978021978, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI41352693, Length=459, Percent_Identity=24.1830065359477, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=60, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1787531, Length=447, Percent_Identity=25.0559284116331, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=25.7309941520468, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367097, Length=340, Percent_Identity=25, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=364, Percent_Identity=29.3956043956044, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=29.3150684931507, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=312, Percent_Identity=29.4871794871795, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=454, Percent_Identity=25.3303964757709, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=57.1125265392781, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6320440, Length=464, Percent_Identity=27.5862068965517, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322261, Length=398, Percent_Identity=28.1407035175879, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=27.6243093922652, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.2251308900524, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=27.2251308900524, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=27.2251308900524, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=26.7955801104972, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645686, Length=462, Percent_Identity=25.5411255411255, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_BACA2 (A7Z7B6)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422123.1
- ProteinModelPortal:   A7Z7B6
- SMR:   A7Z7B6
- STRING:   A7Z7B6
- EnsemblBacteria:   EBBACT00000007863
- GeneID:   5462133
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_025320
- eggNOG:   COG0065
- GeneTree:   EBGT00050000000860
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   BAMY326423:RBAM_025320-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 52129; Mature: 52129

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRTIIEKIWDRHVVKSGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFA
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHE
TMDHNIPTVNRFDIKDEVAKRQVTALERNCEEFGVRLADLHSADQGIVHVVGPELGLTLP
EECCCCCCCEECCCHHHHHHHHHHHHHHCHHHHCCEEEHHCCCCCCEEEEECCCCCEECC
GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEIRVDGTLQKGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCH
KDVILSVIGKYGVKFGTGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDDTTI
HHHHHHHHHHHCCCCCCCEEEEECHHHHHCCCCHHHHEEEECCHHCCCCCCCCCCCCCHH
EYCKNRKYAPKGEEFEKAAAEWKALKTDPGAVYDHSIVLDGSEISPMVTWGVNPGMVLPV
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCEEEECCCCCEEEEE
DAQIPAPETIDSEDGKKEAIRAYEYMGISPNQKIEDIKVEHVFIGSCTNSRMTDLRQAAD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
MIKGRKVADSVRAIVVPGSQSVKQQAEEEGLHRVFLEAGFEWRESGCSMCLSMNNDVVPE
HHCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCC
GERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV
CHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMPRTIIEKIWDRHVVKSGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFA
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHE
TMDHNIPTVNRFDIKDEVAKRQVTALERNCEEFGVRLADLHSADQGIVHVVGPELGLTLP
EECCCCCCCEECCCHHHHHHHHHHHHHHCHHHHCCEEEHHCCCCCCEEEEECCCCCEECC
GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEIRVDGTLQKGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCH
KDVILSVIGKYGVKFGTGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDDTTI
HHHHHHHHHHHCCCCCCCEEEEECHHHHHCCCCHHHHEEEECCHHCCCCCCCCCCCCCHH
EYCKNRKYAPKGEEFEKAAAEWKALKTDPGAVYDHSIVLDGSEISPMVTWGVNPGMVLPV
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCEEEECCCCCEEEEE
DAQIPAPETIDSEDGKKEAIRAYEYMGISPNQKIEDIKVEHVFIGSCTNSRMTDLRQAAD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
MIKGRKVADSVRAIVVPGSQSVKQQAEEEGLHRVFLEAGFEWRESGCSMCLSMNNDVVPE
HHCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCC
GERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV
CHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA