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The map label for this gene is leuD

Identifier: 154686961

GI number: 154686961

Start: 2653481

End: 2654080

Strand: Reverse

Name: leuD

Synonym: RBAM_025310

Alternate gene names: 154686961

Gene position: 2654080-2653481 (Counterclockwise)

Preceding gene: 154686962

Following gene: 154686960

Centisome position: 67.73

GC content: 42.83

Gene sequence:

>600_bases
ATGGAACCTTTAAAAACACATACGGGAAAAGCGGCCGTCTTAAACCGGATCAACGTCGATACGGACCAGATTATTCCGAA
ACAGTTTTTGAAAAGAATTGAAAGAACAGGATACGGCAGATTCGCGTTTTTTGATTGGAGATACCTTGAGAACGGTGAGC
CTGATCCTGAATTTGAATTAAACCGCAAAGTCTATGAAGGCGCGTCCATATTAATTGCCGGAGAGAATTTCGGCTGCGGA
TCATCCCGTGAGCACGCTCCTTGGGCGCTGGATGATTACGGCTTCAAAATCATCATTGCTCCGTCATTTGCCGATATTTT
TCATCAAAACTGTTTTAAAAACGGTATGCTTCCCATCCGTATGCCGTATGATCAGTGGAAAAAACTCGCCGGTCAATATG
AAAATCAGTCTCTTAACATGACGGTTGACTTGGAAAACCAGCTCATTCACGACAGTGAAGGAAAAGAGATTTCATTTGAA
GTCGATCCTCACTGGAAGGAAATGCTGATAAACGGCTATGATGAAATTTCATTAACGCTTCTGCTGGAAGACGACATACA
GCAGTTTGAATCGAAAAGAAGTTCATGGCTTCAAGCCTGA

Upstream 100 bases:

>100_bases
CAGTCCGGCGATGGCGGCAATGGCAGCCATTCACGGGCATTTCGTCGATGTAAGAAAGTTTTATCAAGAAAAAACAGTTG
TGTAAGGAGTGCGCGAAAAG

Downstream 100 bases:

>100_bases
AAAAAGCCGACCGGCATCTGTCCGGCGGCTTTTTTTACCGTGCGCACCATCCTTTCTTGTGTTTCCTGACGCGTGTTGAT
ACACTTGAAAAAAATAGCGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MEPLKTHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLENGEPDPEFELNRKVYEGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDQWKKLAGQYENQSLNMTVDLENQLIHDSEGKEISFE
VDPHWKEMLINGYDEISLTLLLEDDIQQFESKRSSWLQA

Sequences:

>Translated_199_residues
MEPLKTHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLENGEPDPEFELNRKVYEGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDQWKKLAGQYENQSLNMTVDLENQLIHDSEGKEISFE
VDPHWKEMLINGYDEISLTLLLEDDIQQFESKRSSWLQA
>Mature_199_residues
MEPLKTHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLENGEPDPEFELNRKVYEGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDQWKKLAGQYENQSLNMTVDLENQLIHDSEGKEISFE
VDPHWKEMLINGYDEISLTLLLEDDIQQFESKRSSWLQA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=199, Percent_Identity=49.2462311557789, Blast_Score=188, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=46.7336683417085, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BACA2 (A7Z7B5)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422122.1
- ProteinModelPortal:   A7Z7B5
- SMR:   A7Z7B5
- STRING:   A7Z7B5
- EnsemblBacteria:   EBBACT00000007333
- GeneID:   5462132
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_025310
- eggNOG:   COG0066
- GeneTree:   EBGT00050000002923
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   BAMY326423:RBAM_025310-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23126; Mature: 23126

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLKTHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLENGEPDPEFEL
CCCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
NRKVYEGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIR
CCEEECCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEE
MPYDQWKKLAGQYENQSLNMTVDLENQLIHDSEGKEISFEVDPHWKEMLINGYDEISLTL
CCHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCEEEEEECCCHHHHHHCCCCCEEEEE
LLEDDIQQFESKRSSWLQA
EEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPLKTHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLENGEPDPEFEL
CCCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
NRKVYEGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIR
CCEEECCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEE
MPYDQWKKLAGQYENQSLNMTVDLENQLIHDSEGKEISFEVDPHWKEMLINGYDEISLTL
CCHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCEEEEEECCCHHHHHHCCCCCEEEEE
LLEDDIQQFESKRSSWLQA
EEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA