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The map label for this gene is tig

Identifier: 154686959

GI number: 154686959

Start: 2650868

End: 2652142

Strand: Reverse

Name: tig

Synonym: RBAM_025290

Alternate gene names: 154686959

Gene position: 2652142-2650868 (Counterclockwise)

Preceding gene: 154686960

Following gene: 154686958

Centisome position: 67.68

GC content: 43.14

Gene sequence:

>1275_bases
ATGTCTGTAAAATGGGAAAAACAAGAAGGCAACGAAGGCGTATTAACGGTTGAAGTTGATGCGGATACGTTTAAAACAGC
TCTTGATGATGCATTTAAAAAAGTAGTAAAACAGGTTTCTATTCCTGGATTCCGCAAAGGGAAAGTTCCCCGCGGTTTAT
TCGAACAGCGTTTCGGCGTTGAAGCTCTTTACCAGGATGCTTTAGATATTTTACTTCCTGTAGAATACCCTAAAGCGGTT
GAAGAAGCCGGCATTGAGCCTGTAGACCGCCCTGAAATCGATGTTGAAAAAATCGAAAAAGGCGAAAGCTTGATTTTCAC
TGCAAAAGTGACTGTAAAACCGGAAGTGAAACTTGGCGACTACAAAGGCTTAGGAATCGAAAAAGATGACACTGCCGTTA
CTGACGAAGATGTACAAAACGAACTTAAAGCTCTTCAAGAGCGTCAGGCTGAGCTTGTCGTGAAAGAAGAAGGCGCTGTT
GAAAACGGGGATACAGTCGTTCTTGACTTCGAAGGTTTCGTTGACGGCGAAGCGTTTGAAGGCGGAAAAGCTGAGAACTA
CTCACTTGAAGTAGGATCAGGTTCATTCATCCCGGGCTTCGAAGAGCAGTTAACAGGCCTTGAAGCAGGCGCTGAAAAGG
ATGTTGAAGTAACATTCCCTGAAGAGTACCACGCTGAAGAGCTTGCCGGAAAACCAGCTGTTTTCAAAGTGAAAATTCAT
GAAATCAAAGCGAAAGAGCTTCCTGAGCTTAACGACGAATTCGCAAAAGACATTGACGAAGAAGTTGAAACGCTTGCTGA
ATTAACTGAGAAAACAAAAAAACGCCTTGAAGAAGCAAAAGAAAACGAAGCTGACGCTAAATTGCGTGAAGAGCTTGTTC
TGAAAGCATCTGAAAACGCTGAAGCTGATGTTCCACAAGCGATGATCGACACTGAGCTTGACCGCATGCTGAAAGAATTC
GAACAGCGTCTGCAAATGCAAGGCATGAACCTTGAGCTTTACACTCAATTCTCAGGCCAAGACGAAAACGCGTTAAAAGA
GCAAATGAAAGAAGATGCCGCAAAACGCGTAAAATCAAACCTGACTCTTGAAGCGATCGCTCAAGCTGAAAACCTTGAAG
TAACGGACGAAGAAGTTGAAGCTGAGCTGACTAAAATGGCTGAAGCTTACAACATGCCGGTTGAAAACATCAAGCAGGCA
ATCGGTTCAACTGACGCGATGAAAGAAGATTTAAAAGTTCGAAAAGCAATTGATTTTCTTGTAGAAAACCGTTAA

Upstream 100 bases:

>100_bases
TAATGATTCTGATTCAAAGCGGTTTTTTCTTTGACAAAGCCGCGGATTGCTTTAGAATGGAAGACCGACACAACCTGAAT
AGATTTTTGGAGGGAAACAC

Downstream 100 bases:

>100_bases
TATGGTTCATAATAATAGACATAATAAAACAGGGCGCGATTAATTCGTGCCTTGTTTTGTACAATTATGATATAACTCTT
TTTCTTGGGGCAAGATAGTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSVKWEKQEGNEGVLTVEVDADTFKTALDDAFKKVVKQVSIPGFRKGKVPRGLFEQRFGVEALYQDALDILLPVEYPKAV
EEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGDYKGLGIEKDDTAVTDEDVQNELKALQERQAELVVKEEGAV
ENGDTVVLDFEGFVDGEAFEGGKAENYSLEVGSGSFIPGFEEQLTGLEAGAEKDVEVTFPEEYHAEELAGKPAVFKVKIH
EIKAKELPELNDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENAEADVPQAMIDTELDRMLKEF
EQRLQMQGMNLELYTQFSGQDENALKEQMKEDAAKRVKSNLTLEAIAQAENLEVTDEEVEAELTKMAEAYNMPVENIKQA
IGSTDAMKEDLKVRKAIDFLVENR

Sequences:

>Translated_424_residues
MSVKWEKQEGNEGVLTVEVDADTFKTALDDAFKKVVKQVSIPGFRKGKVPRGLFEQRFGVEALYQDALDILLPVEYPKAV
EEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGDYKGLGIEKDDTAVTDEDVQNELKALQERQAELVVKEEGAV
ENGDTVVLDFEGFVDGEAFEGGKAENYSLEVGSGSFIPGFEEQLTGLEAGAEKDVEVTFPEEYHAEELAGKPAVFKVKIH
EIKAKELPELNDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENAEADVPQAMIDTELDRMLKEF
EQRLQMQGMNLELYTQFSGQDENALKEQMKEDAAKRVKSNLTLEAIAQAENLEVTDEEVEAELTKMAEAYNMPVENIKQA
IGSTDAMKEDLKVRKAIDFLVENR
>Mature_423_residues
SVKWEKQEGNEGVLTVEVDADTFKTALDDAFKKVVKQVSIPGFRKGKVPRGLFEQRFGVEALYQDALDILLPVEYPKAVE
EAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGDYKGLGIEKDDTAVTDEDVQNELKALQERQAELVVKEEGAVE
NGDTVVLDFEGFVDGEAFEGGKAENYSLEVGSGSFIPGFEEQLTGLEAGAEKDVEVTFPEEYHAEELAGKPAVFKVKIHE
IKAKELPELNDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENAEADVPQAMIDTELDRMLKEFE
QRLQMQGMNLELYTQFSGQDENALKEQMKEDAAKRVKSNLTLEAIAQAENLEVTDEEVEAELTKMAEAYNMPVENIKQAI
GSTDAMKEDLKVRKAIDFLVENR

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=404, Percent_Identity=37.1287128712871, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BACA2 (A7Z7B3)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422120.1
- ProteinModelPortal:   A7Z7B3
- STRING:   A7Z7B3
- EnsemblBacteria:   EBBACT00000004598
- GeneID:   5463239
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_025290
- eggNOG:   COG0544
- GeneTree:   EBGT00050000000344
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   BAMY326423:RBAM_025290-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47416; Mature: 47284

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKWEKQEGNEGVLTVEVDADTFKTALDDAFKKVVKQVSIPGFRKGKVPRGLFEQRFGV
CCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EALYQDALDILLPVEYPKAVEEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGD
HHHHHHHHHHEECCCCHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEEEECCCEEECC
YKGLGIEKDDTAVTDEDVQNELKALQERQAELVVKEEGAVENGDTVVLDFEGFVDGEAFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKAENYSLEVGSGSFIPGFEEQLTGLEAGAEKDVEVTFPEEYHAEELAGKPAVFKVKIH
CCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCCEEEEEEE
EIKAKELPELNDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENA
HHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
EADVPQAMIDTELDRMLKEFEQRLQMQGMNLELYTQFSGQDENALKEQMKEDAAKRVKSN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
LTLEAIAQAENLEVTDEEVEAELTKMAEAYNMPVENIKQAIGSTDAMKEDLKVRKAIDFL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
VENR
HCCC
>Mature Secondary Structure 
SVKWEKQEGNEGVLTVEVDADTFKTALDDAFKKVVKQVSIPGFRKGKVPRGLFEQRFGV
CCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EALYQDALDILLPVEYPKAVEEAGIEPVDRPEIDVEKIEKGESLIFTAKVTVKPEVKLGD
HHHHHHHHHHEECCCCHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEEEECCCEEECC
YKGLGIEKDDTAVTDEDVQNELKALQERQAELVVKEEGAVENGDTVVLDFEGFVDGEAFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKAENYSLEVGSGSFIPGFEEQLTGLEAGAEKDVEVTFPEEYHAEELAGKPAVFKVKIH
CCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCCEEEEEEE
EIKAKELPELNDEFAKDIDEEVETLAELTEKTKKRLEEAKENEADAKLREELVLKASENA
HHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
EADVPQAMIDTELDRMLKEFEQRLQMQGMNLELYTQFSGQDENALKEQMKEDAAKRVKSN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
LTLEAIAQAENLEVTDEEVEAELTKMAEAYNMPVENIKQAIGSTDAMKEDLKVRKAIDFL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
VENR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA