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The map label for this gene is hemC

Identifier: 154686951

GI number: 154686951

Start: 2640518

End: 2641459

Strand: Reverse

Name: hemC

Synonym: RBAM_025210

Alternate gene names: 154686951

Gene position: 2641459-2640518 (Counterclockwise)

Preceding gene: 154686952

Following gene: 154686950

Centisome position: 67.41

GC content: 49.36

Gene sequence:

>942_bases
ATGAGAACGATTAAGGTAGGTTCCAGACGGAGCAAACTGGCTATGACCCAGACAAAATGGGTGATTAACAAATTGAAGGA
ATTGAACCCTTCCTATAACTTTGAAATCCGGGAGATTGTCACAAAAGGCGACCGCATCCTTGATGTTACACTGTCAAAAG
TGGGCGGCAAAGGTTTGTTTGTCAAAGAGATTGAACAGGCGCTGCTGCATCAGGAGATTGATATGGCGGTTCACAGCATG
AAAGACATGCCCGCCGTTTTGCCTGAGGGTCTTGTCATAGGCTGCATACCGAAACGTGAGGATCCGCGTGATGCGCTGAT
TTCAAAGGGGCATCAAAAGCTGGACGGCATAAAAGAAGGAGGCATCATCGGCACCAGCAGTTTAAGAAGAAGCGCCCAGA
TTCTTGCTGAACGCCCTGATCTTACAATTAAGTGGATCAGAGGCAATATTGATACGAGACTTCAAAAGCTTGAAACGGAA
GATTACGATGCTATTATTTTAGCGGCTGCGGGACTTTCGAGAATGGGCTGGAAAGATGATGTTGTATCTGAATTTCTCGA
ACCGGACCGCTGTCTGCCTGCGGTCGGCCAAGGCGCTCTTGCCATTGAATGCAGAGAGTCGGATGAGGAGCTTTTGAAGC
TGTTTGCGCAATTTACCGATGAGTATACGCAGCGCACGGTGGCAGCCGAACGGGCTTTCTTACACGCGATGGAAGGCGGC
TGCCAAGTGCCGATAGCCGGGTATGCGTCCGTCAATGACCGGGATGAGGTTGAATTGGTCGGTCTTGTCGCGTCTCCGGA
CGGAAAGACGATTTATAAAGAAACAGCGGCGGGACATGATCCGGAAGAAATCGGAAAACGCTGTGCGGCGATGATGTCTG
AAAAAGGAGCCAAAGCATTAATCGACAGTGTAAAACAGGAGCTCGATCAGGATGGCAAATGA

Upstream 100 bases:

>100_bases
TTTCTCGTGCTGATGATTAATTACTTCCTGCTCGGAAGCCTGTCGCAATTCCATTGGTTCAGTTAACGCACGCGCCCAAA
CAGATTCGGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
CCTTCCTTTACAAGGAAAGACCGTGCTCGTCACCCGGAACAAAAGACAGGCCGCACCGTTCCGAAAAAAAGTGGAGGCGC
TCGGCGGAACGGCTGTTTCC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRTIKVGSRRSKLAMTQTKWVINKLKELNPSYNFEIREIVTKGDRILDVTLSKVGGKGLFVKEIEQALLHQEIDMAVHSM
KDMPAVLPEGLVIGCIPKREDPRDALISKGHQKLDGIKEGGIIGTSSLRRSAQILAERPDLTIKWIRGNIDTRLQKLETE
DYDAIILAAAGLSRMGWKDDVVSEFLEPDRCLPAVGQGALAIECRESDEELLKLFAQFTDEYTQRTVAAERAFLHAMEGG
CQVPIAGYASVNDRDEVELVGLVASPDGKTIYKETAAGHDPEEIGKRCAAMMSEKGAKALIDSVKQELDQDGK

Sequences:

>Translated_313_residues
MRTIKVGSRRSKLAMTQTKWVINKLKELNPSYNFEIREIVTKGDRILDVTLSKVGGKGLFVKEIEQALLHQEIDMAVHSM
KDMPAVLPEGLVIGCIPKREDPRDALISKGHQKLDGIKEGGIIGTSSLRRSAQILAERPDLTIKWIRGNIDTRLQKLETE
DYDAIILAAAGLSRMGWKDDVVSEFLEPDRCLPAVGQGALAIECRESDEELLKLFAQFTDEYTQRTVAAERAFLHAMEGG
CQVPIAGYASVNDRDEVELVGLVASPDGKTIYKETAAGHDPEEIGKRCAAMMSEKGAKALIDSVKQELDQDGK
>Mature_313_residues
MRTIKVGSRRSKLAMTQTKWVINKLKELNPSYNFEIREIVTKGDRILDVTLSKVGGKGLFVKEIEQALLHQEIDMAVHSM
KDMPAVLPEGLVIGCIPKREDPRDALISKGHQKLDGIKEGGIIGTSSLRRSAQILAERPDLTIKWIRGNIDTRLQKLETE
DYDAIILAAAGLSRMGWKDDVVSEFLEPDRCLPAVGQGALAIECRESDEELLKLFAQFTDEYTQRTVAAERAFLHAMEGG
CQVPIAGYASVNDRDEVELVGLVASPDGKTIYKETAAGHDPEEIGKRCAAMMSEKGAKALIDSVKQELDQDGK

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=343, Percent_Identity=42.2740524781341, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI66933009, Length=343, Percent_Identity=42.2740524781341, Blast_Score=247, Evalue=1e-65,
Organism=Escherichia coli, GI48994974, Length=304, Percent_Identity=46.3815789473684, Blast_Score=258, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6319996, Length=319, Percent_Identity=38.5579937304075, Blast_Score=202, Evalue=4e-53,
Organism=Drosophila melanogaster, GI20130425, Length=281, Percent_Identity=45.9074733096085, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_BACA2 (A7Z7A5)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422112.1
- ProteinModelPortal:   A7Z7A5
- SMR:   A7Z7A5
- STRING:   A7Z7A5
- EnsemblBacteria:   EBBACT00000006456
- GeneID:   5460785
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_025210
- eggNOG:   COG0181
- GeneTree:   EBGT00050000001150
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   BAMY326423:RBAM_025210-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34545; Mature: 34545

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIKVGSRRSKLAMTQTKWVINKLKELNPSYNFEIREIVTKGDRILDVTLSKVGGKGLF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEHHHCCCCCCC
VKEIEQALLHQEIDMAVHSMKDMPAVLPEGLVIGCIPKREDPRDALISKGHQKLDGIKEG
HHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEEEECCCCCCHHHHHHHCCHHHHCCCCCC
GIIGTSSLRRSAQILAERPDLTIKWIRGNIDTRLQKLETEDYDAIILAAAGLSRMGWKDD
CEECHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEECCHHHCCCCHH
VVSEFLEPDRCLPAVGQGALAIECRESDEELLKLFAQFTDEYTQRTVAAERAFLHAMEGG
HHHHHCCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CQVPIAGYASVNDRDEVELVGLVASPDGKTIYKETAAGHDPEEIGKRCAAMMSEKGAKAL
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEHHHHCCCCCHHHHHHHHHHHHHCCCHHHH
IDSVKQELDQDGK
HHHHHHHHCCCCC
>Mature Secondary Structure
MRTIKVGSRRSKLAMTQTKWVINKLKELNPSYNFEIREIVTKGDRILDVTLSKVGGKGLF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEHHHCCCCCCC
VKEIEQALLHQEIDMAVHSMKDMPAVLPEGLVIGCIPKREDPRDALISKGHQKLDGIKEG
HHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEEEECCCCCCHHHHHHHCCHHHHCCCCCC
GIIGTSSLRRSAQILAERPDLTIKWIRGNIDTRLQKLETEDYDAIILAAAGLSRMGWKDD
CEECHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEECCHHHCCCCHH
VVSEFLEPDRCLPAVGQGALAIECRESDEELLKLFAQFTDEYTQRTVAAERAFLHAMEGG
HHHHHCCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CQVPIAGYASVNDRDEVELVGLVASPDGKTIYKETAAGHDPEEIGKRCAAMMSEKGAKAL
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEHHHHCCCCCHHHHHHHHHHHHHCCCHHHH
IDSVKQELDQDGK
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA