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The map label for this gene is queA

Identifier: 154686913

GI number: 154686913

Start: 2604484

End: 2605512

Strand: Reverse

Name: queA

Synonym: RBAM_024830

Alternate gene names: 154686913

Gene position: 2605512-2604484 (Counterclockwise)

Preceding gene: 154686914

Following gene: 154686912

Centisome position: 66.49

GC content: 49.27

Gene sequence:

>1029_bases
ATGAAAGTCGATTTATTTGATTTTGAACTGCCTGAACGCCTCATTGCCCAAGTGCCGCTTAAAGAGCGTGACGCATCGCG
GCTGATGGTGCTCGACAAACAGACGGGCGGGCTGACGGACAGTTCATTTAAAGAGATTGTAAGCTTTTTTAATGAAGGGG
ATTGCCTCGTCTTGAACAATACCCGTGTGCTGCCTGCAAGATTATTCGGCACAAAAGAAGATACGGGAGCGAAAGTGGAG
CTTCTGTTATTAAAACAGGAAGAAAATGATACATGGGAAACGCTCGTAAAGCCGGCGAAACGCGTAAAAAAAGGAACGGT
CCTGACATTTGGCGACGGCCGGCTGACAGCGGTCTGCACGGAGGAGCTTGATCACGGAGGCAGAAAAATAGAATTCCGGT
ATGACGGGATTTTTTACGAAGTGCTTGAATCACTCGGTGAAATGCCTCTGCCCCCTTATATTAAAGAGCAGCTTGATGAT
AAAGAACGGTATCAGACCGTCTTTTCAAAAGAAATCGGATCTGCGGCAGCTCCGACGGCCGGACTTCATTTTACGGAAGA
GATTCTGGACGAGCTGAAGCAAAAGGGTGTGCGGATTGAATTTATCACACTTCATGTGGGACTCGGCACATTCCGCCCGG
TCAGTGCGGATGATGTGGAAGAGCATAACATGCACGCGGAATTTTATGAAATGACGGAAGAAACCGCCGCCTCTTTAAAC
GAGGTCAGAAAGGCGGGAGGGCGCATTGTGTCGGTCGGGACGACATCCACGCGCACCCTGGAAACCATTGCCGGCGAGCA
TGACGGCGAATTTACAGCTTCAAGCGGCTGGACGTCCATCTTCATTTATCCGGGATATGAATTTAAGGCGATTGACGGCA
TGATCACCAATTTTCATCTGCCGAAATCTTCCCTTATCATGCTCGTTTCTGCGCTTGCCGGCAGAGAGCATGTTCTTTCC
GCCTACCGTCATGCGGTAGAAGAGGAATATCGTTTCTTCAGCTTTGGCGACGCCATGCTGATTATCTAA

Upstream 100 bases:

>100_bases
TTTCCCGTTGTCACTTGTATTGTGATCAGTGTGATACTATCCATTCTGCTTAATTTTTTCGGCAGAATGAAATAACAATA
GACAGAATTAAGGTGAAATG

Downstream 100 bases:

>100_bases
TTTGAATGACAGGAGGCCTCATACAAAGTGGCTGAACAACCGATACGTTATGAATTTATAAAGGAATGCAAACAAACGGG
AGCGCGCCTGGGCAGAGTCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKVDLFDFELPERLIAQVPLKERDASRLMVLDKQTGGLTDSSFKEIVSFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVE
LLLLKQEENDTWETLVKPAKRVKKGTVLTFGDGRLTAVCTEELDHGGRKIEFRYDGIFYEVLESLGEMPLPPYIKEQLDD
KERYQTVFSKEIGSAAAPTAGLHFTEEILDELKQKGVRIEFITLHVGLGTFRPVSADDVEEHNMHAEFYEMTEETAASLN
EVRKAGGRIVSVGTTSTRTLETIAGEHDGEFTASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGREHVLS
AYRHAVEEEYRFFSFGDAMLII

Sequences:

>Translated_342_residues
MKVDLFDFELPERLIAQVPLKERDASRLMVLDKQTGGLTDSSFKEIVSFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVE
LLLLKQEENDTWETLVKPAKRVKKGTVLTFGDGRLTAVCTEELDHGGRKIEFRYDGIFYEVLESLGEMPLPPYIKEQLDD
KERYQTVFSKEIGSAAAPTAGLHFTEEILDELKQKGVRIEFITLHVGLGTFRPVSADDVEEHNMHAEFYEMTEETAASLN
EVRKAGGRIVSVGTTSTRTLETIAGEHDGEFTASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGREHVLS
AYRHAVEEEYRFFSFGDAMLII
>Mature_342_residues
MKVDLFDFELPERLIAQVPLKERDASRLMVLDKQTGGLTDSSFKEIVSFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVE
LLLLKQEENDTWETLVKPAKRVKKGTVLTFGDGRLTAVCTEELDHGGRKIEFRYDGIFYEVLESLGEMPLPPYIKEQLDD
KERYQTVFSKEIGSAAAPTAGLHFTEEILDELKQKGVRIEFITLHVGLGTFRPVSADDVEEHNMHAEFYEMTEETAASLN
EVRKAGGRIVSVGTTSTRTLETIAGEHDGEFTASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGREHVLS
AYRHAVEEEYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=46.242774566474, Blast_Score=314, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BACA2 (A7Z767)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422074.1
- ProteinModelPortal:   A7Z767
- SMR:   A7Z767
- STRING:   A7Z767
- EnsemblBacteria:   EBBACT00000005764
- GeneID:   5462892
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_024830
- eggNOG:   COG0809
- GeneTree:   EBGT00050000002867
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   BAMY326423:RBAM_024830-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38393; Mature: 38393

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDLFDFELPERLIAQVPLKERDASRLMVLDKQTGGLTDSSFKEIVSFFNEGDCLVLNN
CEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVLPARLFGTKEDTGAKVELLLLKQEENDTWETLVKPAKRVKKGTVLTFGDGRLTAVCT
CEEEEHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEE
EELDHGGRKIEFRYDGIFYEVLESLGEMPLPPYIKEQLDDKERYQTVFSKEIGSAAAPTA
HHHHCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
GLHFTEEILDELKQKGVRIEFITLHVGLGTFRPVSADDVEEHNMHAEFYEMTEETAASLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
EVRKAGGRIVSVGTTSTRTLETIAGEHDGEFTASSGWTSIFIYPGYEFKAIDGMITNFHL
HHHHCCCEEEEECCCCCHHHHHHCCCCCCCEEECCCCEEEEEECCCEEEECCCEEEEECC
PKSSLIMLVSALAGREHVLSAYRHAVEEEYRFFSFGDAMLII
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEECCEEEEC
>Mature Secondary Structure
MKVDLFDFELPERLIAQVPLKERDASRLMVLDKQTGGLTDSSFKEIVSFFNEGDCLVLNN
CEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEC
TRVLPARLFGTKEDTGAKVELLLLKQEENDTWETLVKPAKRVKKGTVLTFGDGRLTAVCT
CEEEEHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEE
EELDHGGRKIEFRYDGIFYEVLESLGEMPLPPYIKEQLDDKERYQTVFSKEIGSAAAPTA
HHHHCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
GLHFTEEILDELKQKGVRIEFITLHVGLGTFRPVSADDVEEHNMHAEFYEMTEETAASLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
EVRKAGGRIVSVGTTSTRTLETIAGEHDGEFTASSGWTSIFIYPGYEFKAIDGMITNFHL
HHHHCCCEEEEECCCCCHHHHHHCCCCCCCEEECCCCEEEEEECCCEEEECCCEEEEECC
PKSSLIMLVSALAGREHVLSAYRHAVEEEYRFFSFGDAMLII
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA