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The map label for this gene is tgt

Identifier: 154686912

GI number: 154686912

Start: 2603311

End: 2604456

Strand: Reverse

Name: tgt

Synonym: RBAM_024820

Alternate gene names: 154686912

Gene position: 2604456-2603311 (Counterclockwise)

Preceding gene: 154686913

Following gene: 154686911

Centisome position: 66.46

GC content: 49.48

Gene sequence:

>1146_bases
GTGGCTGAACAACCGATACGTTATGAATTTATAAAGGAATGCAAACAAACGGGAGCGCGCCTGGGCAGAGTCCATACGCC
CCACGGCTCCTTTGAGACGCCTGTATTTATGCCGGTCGGCACGCTTGCTACGGTAAAGACGATGTCTCCGGACGAGCTGA
AATCTATGGATGCGGGCATTATTTTAAGCAATACGTACCATTTATGGCTCCGCCCGGGACATGACATTGTAAAAGAAGCG
GGCGGGCTTCATAAGTTTATGAACTGGGACCGCGCGATTTTAACGGATTCCGGCGGTTTTCAGGTCTTTTCATTGAGCAA
GTTTCGTAATATAGAAGAAGAAGGCGTTCATTTCCGCAATCACTTAAACGGAGACAAGCTGTTTTTATCTCCGGAAAAAG
CGATGGAAATTCAAAACGCCCTCGGCTCTGATATCATGATGGCGTTTGATGAGTGTCCGCCGTATCCGGCAGAGTATGAT
TACATGAAACGCTCTGTGGAACGGACCAGCCGCTGGGCGGAACGCTGCCTGGAGGCGCACGGGCGCTCAGATGAGCAGGG
GCTGTTCGGAATCGTACAGGGCGGAGAATACGAAGACCTGCGGACACAAAGTGCAAAAGACTTGGTTTCGCTTGATTTTC
CGGGGTATGCCATAGGCGGGTTATCTGTCGGCGAGCCGAAGCACGTCATGAACCGAGTGCTTGAGTTTACAACTCCGCTT
CTACCGAAGGACAAGCCGAGATATTTAATGGGTGTGGGTTCACCGGATGCCTTAATTGACGGCGCCATCCGCGGCGTGGA
TATGTTTGACTGCGTACTGCCGACCCGGATTGCCCGAAACGGCACGGTGTTTACGAATGAAGGGCGTCTGAATATGAAAA
ACGCCAAATATGAGCGTGATTTCCGTCCGATTGATGAAGAGTGCAGCTGCCATACATGCAAAAATTACTCCCGCGCTTAC
ATCAGACATTTGATCCGCTGTAATGAAACGTTTGGAATTCGATTAACAACTTACCATAACCTTCATTTTCTGTTACACTT
AATGGAGCAGGTCAGACAAGCCATCCGGGAGGATCGTCTTGGTGATTTCCGAGAAGAGTTCTTTGAGCGTTACGGCTATA
ATAAGCCAAATGCGAAAAGCTTCTAA

Upstream 100 bases:

>100_bases
TTCCGCCTACCGTCATGCGGTAGAAGAGGAATATCGTTTCTTCAGCTTTGGCGACGCCATGCTGATTATCTAATTTGAAT
GACAGGAGGCCTCATACAAA

Downstream 100 bases:

>100_bases
ATAAAACCATTCATGAAAGGAGGGAAACAGTTGGGTACTTTAGGCACAATCGTTCCTATTATTTTAATGTTTGCAGTGCT
TTATTTCCTGCTCATCCGTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAEQPIRYEFIKECKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPDELKSMDAGIILSNTYHLWLRPGHDIVKEA
GGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYD
YMKRSVERTSRWAERCLEAHGRSDEQGLFGIVQGGEYEDLRTQSAKDLVSLDFPGYAIGGLSVGEPKHVMNRVLEFTTPL
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTNEGRLNMKNAKYERDFRPIDEECSCHTCKNYSRAY
IRHLIRCNETFGIRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF

Sequences:

>Translated_381_residues
MAEQPIRYEFIKECKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPDELKSMDAGIILSNTYHLWLRPGHDIVKEA
GGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYD
YMKRSVERTSRWAERCLEAHGRSDEQGLFGIVQGGEYEDLRTQSAKDLVSLDFPGYAIGGLSVGEPKHVMNRVLEFTTPL
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTNEGRLNMKNAKYERDFRPIDEECSCHTCKNYSRAY
IRHLIRCNETFGIRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF
>Mature_380_residues
AEQPIRYEFIKECKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPDELKSMDAGIILSNTYHLWLRPGHDIVKEAG
GLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDY
MKRSVERTSRWAERCLEAHGRSDEQGLFGIVQGGEYEDLRTQSAKDLVSLDFPGYAIGGLSVGEPKHVMNRVLEFTTPLL
PKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTNEGRLNMKNAKYERDFRPIDEECSCHTCKNYSRAYI
RHLIRCNETFGIRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=368, Percent_Identity=45.1086956521739, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI13375872, Length=286, Percent_Identity=26.5734265734266, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=57.3816155988858, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=49.8607242339833, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25146502, Length=245, Percent_Identity=23.265306122449, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=46.505376344086, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=26.5625, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BACA2 (A7Z766)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422073.1
- ProteinModelPortal:   A7Z766
- SMR:   A7Z766
- STRING:   A7Z766
- EnsemblBacteria:   EBBACT00000005633
- GeneID:   5462891
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_024820
- eggNOG:   COG0343
- GeneTree:   EBGT00050000001431
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   BAMY326423:RBAM_024820-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43666; Mature: 43535

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPIRYEFIKECKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPDELKSMDAGI
CCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCHHEEECCCHHHHHHCCCCE
ILSNTYHLWLRPGHDIVKEAGGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRN
EEECCEEEEEECCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEEHHHCCCHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKRSVERTSRWAERCLEAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GRSDEQGLFGIVQGGEYEDLRTQSAKDLVSLDFPGYAIGGLSVGEPKHVMNRVLEFTTPL
CCCCCCCCEEEECCCCCHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCC
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTNEGRLNMKNAKYERD
CCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCEECCCCCCCCC
FRPIDEECSCHTCKNYSRAYIRHLIRCNETFGIRLTTYHNLHFLLHLMEQVRQAIREDRL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCC
GDFREEFFERYGYNKPNAKSF
CHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AEQPIRYEFIKECKQTGARLGRVHTPHGSFETPVFMPVGTLATVKTMSPDELKSMDAGI
CCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEEECCCHHEEECCCHHHHHHCCCCE
ILSNTYHLWLRPGHDIVKEAGGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRN
EEECCEEEEEECCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEEHHHCCCHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKRSVERTSRWAERCLEAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GRSDEQGLFGIVQGGEYEDLRTQSAKDLVSLDFPGYAIGGLSVGEPKHVMNRVLEFTTPL
CCCCCCCCEEEECCCCCHHHHHHHHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCC
LPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTNEGRLNMKNAKYERD
CCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCEECCCCCCCCC
FRPIDEECSCHTCKNYSRAYIRHLIRCNETFGIRLTTYHNLHFLLHLMEQVRQAIREDRL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCC
GDFREEFFERYGYNKPNAKSF
CHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA