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The map label for this gene is spoIVB [H]

Identifier: 154686687

GI number: 154686687

Start: 2392959

End: 2394239

Strand: Reverse

Name: spoIVB [H]

Synonym: RBAM_022560

Alternate gene names: 154686687

Gene position: 2394239-2392959 (Counterclockwise)

Preceding gene: 154686688

Following gene: 154686686

Centisome position: 61.1

GC content: 44.89

Gene sequence:

>1281_bases
ATGCACGATAACATGAGGAAAGCAATAGGTGTAATTCTCCTTGTTTCTTTACTAAGTGTAGGCTTTTTTAAGCCATTTAA
AGAATATTTACTGATACCGTCAGAAATGAACGTTTTTGAAAACCAGACACAGGCCGTTCCGGTTAATGCATCAATAAGCG
CTGAAAAGGGCGAATTATCAGAAGCCTTTACGCTGAAAGAAGAGCACGGCCATGCAAACATAACAGGGCAAACAAAAGGC
CGGTCAGAAGTAGTATATGATCTTGCCGGATTTCCCATTAAAAAAACGAAAGTGCGTGTCCTTCCAGAATTGAAGGTCAT
TCCGGGCGGACAGTCCATCGGTGTAAAACTGCATTCAGTCGGTGTTTTGGTCGTCGGTTTTCACCAGGTGATGACAAAGG
ATGGGAAAAAGTCTCCGGGAGAACAAGCGGGAATTGAATCTGGCGACATCATTATTAAAATGAACGGACATAAAATTGAT
AAAATGAGCGACATCGCCCCGTTTATTCAAAAAGCGGGCAAAACCGGTGAATCTTTGGATTTATTGGTGAAACGCGATAA
GCAAAAAATCAAAACAAAGCTGATTCCGACAAAGGACGAAGCCGAGGGGAAATATCGAATCGGCCTTTATATCCGCGATT
CGGCAGCCGGCATCGGCACCATGACTTTTTTTGAACCGAAAACAAAAAAATACGGCGCATTGGGCCACGTCATCTCTGAT
ATGGATACAAAAAAACCGATTGTTGTAGAAGACGGAGAAATCGTCCGTTCAACTGTAACATCGATTGAAAAAGGAAAAGG
CGGCAATCCCGGCGAAAAACTGGCAAGGTTTTCGTCAGAACGGAAAACGATCGGCGATATTAACCGCAACAGCCCGTTCG
GCATCTTCGGGACACTGCATCAGCCGATTAAAAACAACATTTCCGACAAAGCGCTTCCGATTGCTTTGTCAAATGAAGTA
AAAAAAGGACCTGCGCAAATGCTGACGGTTATCGAAAACGATAAAGTAGAGAAATTTGATATTGAAATCGTCAGCACCAC
GCCGCAAAAATTCCCGGCGACAAAGGGAATGGTGTTGAAAGTGACAGATCCGAAACTGCTGAAAAGAACCGGCGGAATCG
TTCAGGGGATGAGCGGAAGCCCGATCATCCAGAACGGAAAGGTCGTCGGAGCCGTAACCCACGTATTTGTCAATGATCCG
ACAAGCGGCTACGGCGTTCATATCGAATGGATGCTGTCTGAAGCGGGTGTTGATGTTTACGGGAAAGACAAAGCAAGCTG
A

Upstream 100 bases:

>100_bases
TTCGTATGCCGGTTATAAATCAAGCGCAGGAAGGCAAAAGTATAGATGTAGCAGAAAAAGAAAGTGGTGTGGGATAGGAG
CGAGGAGAGTGAAGGAATGA

Downstream 100 bases:

>100_bases
CTGCCGGGTGATCCGGCAGTTTTTTTATGTGATGCGTTCTTCACTTCTTTCCTGGATTCATGTAAAATAAGACATGAAGA
TTTTTCGACAAATTCACGTT

Product: SpoIVB

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MHDNMRKAIGVILLVSLLSVGFFKPFKEYLLIPSEMNVFENQTQAVPVNASISAEKGELSEAFTLKEEHGHANITGQTKG
RSEVVYDLAGFPIKKTKVRVLPELKVIPGGQSIGVKLHSVGVLVVGFHQVMTKDGKKSPGEQAGIESGDIIIKMNGHKID
KMSDIAPFIQKAGKTGESLDLLVKRDKQKIKTKLIPTKDEAEGKYRIGLYIRDSAAGIGTMTFFEPKTKKYGALGHVISD
MDTKKPIVVEDGEIVRSTVTSIEKGKGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLHQPIKNNISDKALPIALSNEV
KKGPAQMLTVIENDKVEKFDIEIVSTTPQKFPATKGMVLKVTDPKLLKRTGGIVQGMSGSPIIQNGKVVGAVTHVFVNDP
TSGYGVHIEWMLSEAGVDVYGKDKAS

Sequences:

>Translated_426_residues
MHDNMRKAIGVILLVSLLSVGFFKPFKEYLLIPSEMNVFENQTQAVPVNASISAEKGELSEAFTLKEEHGHANITGQTKG
RSEVVYDLAGFPIKKTKVRVLPELKVIPGGQSIGVKLHSVGVLVVGFHQVMTKDGKKSPGEQAGIESGDIIIKMNGHKID
KMSDIAPFIQKAGKTGESLDLLVKRDKQKIKTKLIPTKDEAEGKYRIGLYIRDSAAGIGTMTFFEPKTKKYGALGHVISD
MDTKKPIVVEDGEIVRSTVTSIEKGKGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLHQPIKNNISDKALPIALSNEV
KKGPAQMLTVIENDKVEKFDIEIVSTTPQKFPATKGMVLKVTDPKLLKRTGGIVQGMSGSPIIQNGKVVGAVTHVFVNDP
TSGYGVHIEWMLSEAGVDVYGKDKAS
>Mature_426_residues
MHDNMRKAIGVILLVSLLSVGFFKPFKEYLLIPSEMNVFENQTQAVPVNASISAEKGELSEAFTLKEEHGHANITGQTKG
RSEVVYDLAGFPIKKTKVRVLPELKVIPGGQSIGVKLHSVGVLVVGFHQVMTKDGKKSPGEQAGIESGDIIIKMNGHKID
KMSDIAPFIQKAGKTGESLDLLVKRDKQKIKTKLIPTKDEAEGKYRIGLYIRDSAAGIGTMTFFEPKTKKYGALGHVISD
MDTKKPIVVEDGEIVRSTVTSIEKGKGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLHQPIKNNISDKALPIALSNEV
KKGPAQMLTVIENDKVEKFDIEIVSTTPQKFPATKGMVLKVTDPKLLKRTGGIVQGMSGSPIIQNGKVVGAVTHVFVNDP
TSGYGVHIEWMLSEAGVDVYGKDKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 46185; Mature: 46185

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDNMRKAIGVILLVSLLSVGFFKPFKEYLLIPSEMNVFENQTQAVPVNASISAEKGELS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHCCCCEEEEECCCCCCCCCCCC
EAFTLKEEHGHANITGQTKGRSEVVYDLAGFPIKKTKVRVLPELKVIPGGQSIGVKLHSV
HHEEEECCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEECCCEEEECCCCCCCEEEEEC
GVLVVGFHQVMTKDGKKSPGEQAGIESGDIIIKMNGHKIDKMSDIAPFIQKAGKTGESLD
CEEEEEHHHHHCCCCCCCCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHH
LLVKRDKQKIKTKLIPTKDEAEGKYRIGLYIRDSAAGIGTMTFFEPKTKKYGALGHVISD
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHC
MDTKKPIVVEDGEIVRSTVTSIEKGKGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLH
CCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEHHCC
QPIKNNISDKALPIALSNEVKKGPAQMLTVIENDKVEKFDIEIVSTTPQKFPATKGMVLK
HHHHCCCCCCEEEEEECCCCCCCHHHEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEE
VTDPKLLKRTGGIVQGMSGSPIIQNGKVVGAVTHVFVNDPTSGYGVHIEWMLSEAGVDVY
ECCHHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCC
GKDKAS
CCCCCC
>Mature Secondary Structure
MHDNMRKAIGVILLVSLLSVGFFKPFKEYLLIPSEMNVFENQTQAVPVNASISAEKGELS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHCCCCEEEEECCCCCCCCCCCC
EAFTLKEEHGHANITGQTKGRSEVVYDLAGFPIKKTKVRVLPELKVIPGGQSIGVKLHSV
HHEEEECCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEECCCEEEECCCCCCCEEEEEC
GVLVVGFHQVMTKDGKKSPGEQAGIESGDIIIKMNGHKIDKMSDIAPFIQKAGKTGESLD
CEEEEEHHHHHCCCCCCCCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHH
LLVKRDKQKIKTKLIPTKDEAEGKYRIGLYIRDSAAGIGTMTFFEPKTKKYGALGHVISD
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHC
MDTKKPIVVEDGEIVRSTVTSIEKGKGGNPGEKLARFSSERKTIGDINRNSPFGIFGTLH
CCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEHHCC
QPIKNNISDKALPIALSNEVKKGPAQMLTVIENDKVEKFDIEIVSTTPQKFPATKGMVLK
HHHHCCCCCCEEEEEECCCCCCCHHHEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEE
VTDPKLLKRTGGIVQGMSGSPIIQNGKVVGAVTHVFVNDPTSGYGVHIEWMLSEAGVDVY
ECCHHHHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCC
GKDKAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]